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Total Leukocyte Count (TLC) Test Procedure

A Leukocyte (TLC) count is a test that measures the number of white blood cells in your body. This test is often included with a complete blood count (CBC). The term “white blood cell count” is also used more generally to refer to the number of white blood cells in your body.

There are several types of white blood cells, and your blood usually contains a percentage of each type. Sometimes, however, your white blood cell count can fall or rise out of the healthy range.

Total WBC Count Test

Also Known As: WBC CountLeukocyte CountWhite Count, TLC, Leukocyte


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Test Panel: Hemoglobin, Red Blood Cells (RBC), HCT, MCV, MCH, MCHC, Platelets Count, White Blood Cells (WBC), DLC, ESR

TLC Test Procedure:

Test Requirements


  • Take 0.38 ml of Turk’s Colution and 20ul of Blood in a test tube.
  • Mix the blood and Turk’s Solution by shaking with thumb and finger over the end.
  • Fill The Neubauer Chamber by Micropipette.
  • Place the counting chamber on the microscope stage and allow 2-3 min. for the cells to settle.
  • Count the number of white cells in four large corner squares marked “W”.
  • Cells which are touching the left-hand lines or upper lines of the square are included in the count, while cells touching the lower and right margins are excluded.
  • After The Countion celles then calculate the total wbc count for this formula

TLC = Total Cells(4 squares) x 50

Referance Ranges:

Test NameMaleFemale
WBC (TLC)4.0 – 11.0 x 109 /l4.0 – 11.0 x 109 /l

Read more about Leukocyte

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