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Blood Film

Examination of thin blood films is much valuable for the analysis and management of anaemia, infections, and other conditions which produce changes in the appearance of blood cells and differential white cell count. A blood film can describe immediately and at low prices, handy statistics relating to patient’s state.
Thick Blood Films:
The preparation, staining and reporting of thick blood films and colour plates illustrating malaria parasites, trypanosomes, and microfilariae.
Making, Staining and Fixing of Blood Film:
Thin blood film should be made with well mix EDTA blood, within 1 hour of collection of blood. Always wear gloves as a safety precaution.

Peripheral Smear
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Technique For Making Blood a Thin Film:

  1. Make a blood spreader with round and polished sides. A good slide have good round and polished sides. Examine the each side for smooth surface for making a smear.
  2. On the clean and dry side of slide place a small drop of blood and care should be taken to avoid a large drop.
  3. Using a clean and smooth edges of spreader for smear. Draw back the the spreader to touch the blood drop and allow the blood extend along the edge of spreader.
  4. Clean the end of the slide to remove extra blood.
  5. Air dry the slide quickly by waving the slide back and forth and save the slide from dust and insects.
  6. When the slide is fully dried within few minutes fix in absolute methanol.

Qualities of a Well Made Film:

  1. Not too thick, nor too long
  2. Free from lines and holes
  3. Has a smooth tail

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