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Ethylenediaminetetraacetic acid (EDTA)

Ethylenediaminetetraacetic acid (EDTA) is a chemical used for both industrial and medical purposes.


Other names
Diaminoethane-tetraacetic acid , Edetic acid , Ethylenedinitrilo-tetraacetic acid

Abbreviations: EDTA, H4EDTA

Chemical formula: C10H16N2O8


EDTA is used extensively in the analysis of blood. It is an anticoagulant for blood samples for CBC/FBEs, where the EDTA chelates the calcium present in the blood specimen, arresting the coagulation process and preserving blood cell morphology.

EDTA (seqestrene) anticoagulant

Obtain ready-prepared anticoagulated bottles, or prepare as follows:

  • Di-potassium ethylene (diamine-tetra-acetic acid) … 2.5 g
  • Distilled water ……………..…………………………….……….. 25 ml
  • Weigh the chemical, and transfer it to a small glass bottle.
  • Measure 25 ml of water, add to the chemical, and mix to dissolve. Label the bottle.
  • For use, pipette 0.04 ml of the reagent into small bottles marked to hold 2.5 ml of blood.
  • Place the small bottles without tops, on a warm bench for the anticoagulant to dry.Protect from dust and flies.
  • When dry, replace the bottle tops, and store ready for use.

Possible References Used

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