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Albumin Test Procedure

Proteins circulate throughout your blood to help your body maintain fluid balance. Albumin is a type of protein the liver makes. It’s one of the most abundant proteins in your blood.

Albumin Test Procedure

Also Known as: Albumin, ALB, Serum Albumin


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Test Panel: Total Bilirubin, Conjagated Bilirubin, Unconjugated Bilirubin, ALT, AST, ALP, Total Protein, Albumin, Globulin, A/G ratio, GGT,

BCG Method

For the Determination of Albumin in Human Serum or Plasma

Principle :

At a certain pH value, albumin is specifically combined with bromcresol green, to produce a coloured complex, which is photometrically measured

Kit Contents:

  • R 1 :
    • Phosphate buffer pH 4.0 —– 70 mmol/L
    • BCG ———— 0/25 mmol/L
  • Standard: ——- 4.0 g/dL

Test Requirements :

  • Test Tubes
  • Micropipettes
  • Tips
  • Bio-Chemistry analyzer
  • SGPT Test Kit
  • Calibrator

Reagent Preparation and Stability :

All Reagents are Ready to use reagents

Specimen collection and handling:

  1. Non-hemolyzed serum, heparinized or EDTA plasma is recommended.
  2. Stability: 4 weeks at 2 – 8° C.

Analyzer Parameter Required :

Reaction Type End point
Wavelength 630 nm
Cuvette Temp 37°C, 20 – 25°C
Zero Setting reagent blank
Light Path 1 cm

Test Procedure:

Pipette into clean dry test tube labeled as Sample (S), Calibrator Label (C)m Blank as (B) and QC Label (QC) or as your required:

Specimin(B)(S)(C) / (QC)
R11.0 ml (1000 µL ) 1.0 ml (1000 µL ) 1.0 ml (1000 µL )
Sample 10 µL
Calibrator or QC10 µL
Dist. water 10 µL
Mix well and incubate for 3 min at 37 ºC or 5 min at room temperature (20 – 25 °C). After incubation, zero the Photometer with the reagent blank. Read and record the incubated standard and samples.

CALCULATION : With standard or calibrator

Albumin g/dL = ∆A Sample / ∆A Std/Cal x 4(Conc std/cal g/dL)

NOTE: Samples having a very low or high activity show a very low initial absorbance as most of the NADH is consumed prior to the start of the measurement. If this is suspected then dilute the sample and repeat the assay


The procedure is linearity is 7.5 g/dl and depends on KIT.
if the activity exceeds this limits dilute the sample with normal saline (NaCl 0.9 %) and multiply result by dilution factor.


For accuracy it is necessary to run known controls with every assay.

Normal Value :

Serum : 3.5 to 5.3 g/dL.
Note: Each Laboratory or Each Company Kits should establish it’s own normal range.

Related Articles:

Possible References Used

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