Burrow’s stain, also known as Burrow’s method, is a histological staining technique used to highlight specific cellular and tissue components. It is particularly used in the study of skin lesions and dermatopathology.
Uses of Burrow’s stain:
- Dermatopathology: Identifies and differentiates various skin lesions and conditions.
- Histological Analysis: Highlights specific cellular components and tissue structures in biopsy samples.
- Disease Diagnosis: Aids in diagnosing skin diseases and disorders based on staining patterns and tissue morphology.
- Research: Used in research settings to study skin-related diseases and their effects on tissue structure.
- Educational Purposes: Provides clarity in teaching histology and dermatopathology by demonstrating specific staining characteristics.
Composition of Burrow’s stain:
Here is the table showing the composition of Burrow’s stain to make 100 ml:
| Component | Quantity |
|---|---|
| Thionin | 0.02 g |
| Ethanol, absolute | 3 ml |
| Acetic acid, glacial | 3 ml |
| Distilled water | 94 ml |
Preparation of Burrow’s stain:
- Weigh the Thionin: Weigh 0.02 g (20 mg) of thionin. If a precise balance is unavailable, use a wet swab stick or the unburnt end of a match stick to transfer a small amount of powdered thionin.
- Add Ethanol and Acetic Acid: Measure 3 ml of absolute ethanol and 3 ml of glacial acetic acid. Add these to a 100 ml leakproof bottle. Mix until the thionin is completely dissolved.
Caution: Handle ethanol and glacial acetic acid with care. They are flammable and glacial acetic acid is also corrosive and has irritating vapors. Use in a well-ventilated area away from open flames. Do not use mouth-pipettes. - Add Distilled Water: Add 94 ml of distilled water to the bottle and mix well to complete the preparation.
- Label and Store: Label the bottle with the contents and date of preparation. Store the stain at 2–8 °C. Renew the stain every 3 months for optimal performance.
Precautions:
- Thionin: Handle with care; use gloves and lab coat to avoid skin contact and inhalation.
- Ethanol: Highly flammable; keep away from flames and heat. Use in a well-ventilated area.
- Glacial Acetic Acid: Corrosive and flammable; use PPE (gloves, goggles) and work in a well-ventilated area. Avoid inhaling vapors.
- General Safety: Follow all safety protocols, label containers clearly, and store chemicals properly.
- Preparation Area: Keep the area clean, use appropriate containers, and avoid contamination.
- Disposal: Dispose of chemical waste according to institutional or local guidelines; do not pour down the sink.
Uses of in Clinical Laboratories Burrow’s stain:
- Skin Biopsies: Analyzes and differentiates various skin lesions for diagnostic purposes.
- Dermatopathology: Helps in identifying specific skin diseases and conditions through detailed staining of tissue samples.
- Histological Studies: Enhances visualization of cellular and tissue components in histological preparations.
- Disease Diagnosis:Assists in diagnosing dermatological conditions based on staining patterns and morphological details.
- Research: Used in research to study skin diseases and the effects on tissue structure.
⚠️ Disclaimer:
The content on LabTestsGuide.com is for informational and educational purposes only. We do not guarantee the accuracy, completeness, or timeliness of the information provided. Always consult qualified healthcare professionals for medical advice, diagnosis, or treatment. LabTestsGuide.com is not liable for any decisions made based on the information on this site.
