It is used as diluting fluid for blood specimens to count red blood cells under high powder.
- Mercuric chloride – 0.25 gm
- Sodium sulphate – 2.50 gm
- Sodium chloride – 0.50 gm
- Distilled water – 100.0ml
- Final pH ( at 25°C) – 5.9±0.1
Principle And Interpretation:
RBC diluting fluid is isotonic with blood, hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation.
The blood specimen is diluted 1:200 with the RBC diluting fluid and cells are counted under high power (40 x objective) by using a counting chamber.
The number of cells in undiluted blood are calculated and reported as the number of Red cells per cu mm (MI) of whole blood.
Possible References Used