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Evaluating patients with signs and symptoms of scleroderma and other connective tissue diseases in whom the test for antinuclear antibodies is positive.

Antibodies to extractable nuclear antigens (ENAs) are autoantibodies produced when the immune system mistakenly attacks the body’s own tissues. The ENA panel detects these autoantibodies in the blood to help diagnose autoimmune disorders.

Extractable Nuclear Antigens (ENAs) they are more than 100 different soluble nuclear and cytoplasmic antigens. They are known as “extractable” because they can be removed from cell nuclei using saline and represent six main proteins: Ro, La, Sm, RNP, Scl-70, Jo1. Most ENAs are part of spliceosome or nucleosome complexes and are a type of small nuclear ribonucleoprotein (snRNPS). The location in the nucleus and association with spiceosomes or nucleosomes results in these ENAs associating with additional RNA and proteins, such as polymerases. This quality of ENAs often makes it difficult to purify and quantify their presence for clinical use.


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Also Known As: Antibodies to Saline-extracted AntigensAnti-RNPAnti-RibonucleoproteinAnti-U(1)RNPAnti-SmRNPAnti-SSASSA (Ro)Anti-Sjögren Syndrome A ,Anti-SSBSSB (La)Anti-Sjögren Syndrome BAnti-Sm (Sm)Smith AntibodyScl-70Anti-TopoisomeraseScleroderma AntibodiesAnti-Jo-1Antihistidyl Transfer RNA Synthase AntibodiesENA Panel

Why get tested?

To help diagnose and distinguish between autoimmune disorders, as well as to monitor the progression of autoimmune disease.

When Tested?

  • When your antinuclear antibody (ANA) test is positive and you have symptoms that suggest an autoimmune disorder
  • When monitoring the activity of an autoimmune disorder


  • A blood sample drawn from a vein in your arm
  • 3 mL Sample in Plain or Yellow Cap Tube

Test Preparations:

No Need any Preparations for This Test

Reference Values:

  • <1.0 U (negative)
  • or =1.0 U (positive)

Reference values apply to all ages.

Clinical Information:

Scl 70 (topoisomerase 1) is a 100-kD nuclear and nucleolar enzyme. Scl 70 antibodies are considered to be specific for scleroderma (systemic sclerosis) and are found in up to 60% of patients with this connective tissue disease. Scl 70 antibodies are more common in patients with extensive cutaneous involvement and interstitial pulmonary fibrosis, and are considered a poor prognostic sign.

A 4-test ENA panel will include:

Autoantibody TestFormally Known As
Anti-RNPAnti-U(1)RNP, Anti-Ribonucleoprotein
Anti-SmSmith Antibody
Anti-SS-A (Ro)Anti-Sjögren Syndrome A
Anti-SS-B (La)Anti-Sjögren Syndrome B

A 6-test ENA panel will include:

Autoantibody TestFormally Known As
Anti-RNPAnti-U(1)RNP, Anti-Ribonucleoprotein
Anti-SmSmith Antibody
Anti-SS-A (Ro)Anti-Sjögren Syndrome A
Anti-SS-B (La)Anti-Sjögren Syndrome B
Anti-Scl-70Scleroderma Antibodies; anti-topoisomerase
Anti-Jo-1Anti-Histidyl Transfer RNA Synthase Antibodies

When this Test Ordered

An ENA panel is ordered when a person has signs and symptoms that could be due to an autoimmune disorder and has a positive ANA test. The signs and symptoms of autoimmune disorders are highly variable and can affect many different areas of the body. They may include:

  • Fever and persistent fatigue
  • Muscle pain
  • Joint swelling and/or pain
  • Skin rash
  • Hair loss or loss of scalp hair
  • Sensitivity to ultraviolet light
  • Raynaud phenomenon
  • Protein in the urine (proteinuria)
  • Neurologic symptoms such as seizures, depression, psychoses
  • Hemolytic anemia (low red blood cell count) or leukopenia (low white blood cell count)
Autoantibody TestResults That Support an Autoimmune Disorder Diagnosis
Anti-RNPPositive result seen in 95-100% of mixed connective tissue disease (MCTD) cases; may also be positive with lupus and scleroderma
Anti-SmPositive result seen in 30% of those with lupus; very specific antibody marker for this disease
Anti-SS-A (Ro)Positive result seen in 75% of those with Sjögren syndrome; may also be positive with lupus and scleroderma
Anti-SS-B (La)Positive result seen in 60% of those with Sjögren syndrome; may also be positive with lupus and scleroderma; rarely present without anti-SS-A
Anti-Scl-70Positive result seen in 60% of those with scleroderma; highly specific antibody marker for this disease
Anti-Jo-1Positive result seen in 30% of those with polymyositis; may be positive with pulmonary fibrosis

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