Antiglobulin Test Purpose, Procedure, Result Interpretation and Your Test Result Meaning

The antiglobulin test, also known as the Coombs test, is a laboratory test used to detect the presence of antibodies or complement proteins attached to the surface of red blood cells (RBCs). It is primarily used to diagnose and investigate immune-related blood disorders, such as autoimmune hemolytic anemia and hemolytic disease of the newborn.

Also Known as	Coombs Test, Antiglobulin Test, Anti Human Globulin Test
Test Purpose	This Test to done find certain antibodies that cause autoimmune haemolysis of red blood cells (erythrocytes).
Test Preparations	No Need any Preparations
Test Components	Coombs Test Direct Coombs Test Indirect
Specimen	Direct coombs: 3 ML (2 ML Min.) Whole Blood From 1 Lavender Top (EDTA) Tube.  Indirect Coombs: 2 ML (1 ML Min.) Serum From 1 SST.
Stability Room	Direct Coombs: 2 hours Indirect Coombs : 2 hours
Stability Refrigerated	Direct Coombs: 1 week Indirect Coombs : 48 hours
Stability Frozen	Direct Coombs: 2 weeks Indirect Coombs : N/A
Method	Erythrocyte Magnetised Technology
Download Report	Download Report

Types of Coombs Test:

There are two different types of Coombs tests

1. Direct Coombs Test:
   The direct Coombs test (sometimes called the direct antiglobulin test) examines red blood cells to see if they have antibodies attached to them. It is often used to diagnose blood-related conditions, such as autoimmune hemolytic anemia. People with autoimmune hemolytic anemia don’t have enough red blood cells because their bodies destroy them too soon.
2. Indirect Coombs Test:
   The indirect Coombs test tells your provider if there are antibodies present in your bloodstream that could attach to red blood cells. It is used as a screening process to see how you will react to a blood transfusion. An indirect Coombs test is also routinely done as part of prenatal testing.

Principle of Coombs’ Test

Red blood cells coated with complement or IgG antibodies do not directly agglutinate when centrifuged. These cells are said to be IgG or complement sensitized. For agglutination to occur, an additional antibody must be added to the system that reacts with the Fc portion of the IgG antibody, or with the C3b or C3d component of complement. This will form a “bridge” between the antibodies or complement that coats the red blood cells, causing agglutination.

Coombs Test Result Mean

Result	Direct Coombs Test	Indirect Coombs Test
Negative result	Antibodies not attached to Red Blood Cells	Antibodies not loose in Serum
Positive result	Antibodies attached to Red Blood Cells	Antibodies loose in Serum

A Positive Coombs test is seen in

A positive (abnormal) Coombs’ test means you have antibodies that can attack your red blood cells. This can be caused by various conditions, including:

• Autoimmune hemolytic anemia.
• Infectious mononucleosis.
• Chronic lymphocytic leukemia.
• Syphilis.
• Mycoplasma infection, a type of respiratory disease.
• Lupus.
• A negative reaction to a blood transfusion.

Positive test during Pregnancy mean

If you test positive during your pregnancy, it means you need to take steps to protect your baby from Rh sensitization.

Positive Coombs in Baby

Generally, a positive Coombs test result in babies indicates Anemia or Jaundice.

Meaning of Negative Coombs Test Result

A negative (normal) test result is good news. It means that no antibodies against red blood cells were found.

• You can safely receive donor blood during a transfusion.
• You don’t have to worry about Rh sensitization affecting your unborn baby.

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Possible References Used

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