Also Known As: HBV Tests, Hep B, anti-HBs, Hepatitis B Surface Antibody, HBsAg, Hepatitis B Surface Antigen, HBeAg,, Hepatitis B e Antigen, anti-HBc, Hepatitis B Core Antibody, anti-HBc, IgM, anti-HBe, Hepatitis B e Antibody, HBV DNA ,HBV PCR
Why Get Tested?
When To Get Tested?
When you have risk factors for HBV infection or when you have signs and symptoms of hepatitis, such as jaundice or unexplained elevated blood levels of alanine aminotransferase (ALT), a liver-associated enzyme; when you have a condition that requires chemotherapy or drugs that suppress your immune system; when you are being treated for HBV or hepatitis C (HCV); when it is unclear whether you have immunity and your healthcare practitioner is considering giving you the hepatitis B vaccineing you the hepatitis B vaccine.
A blood sample drawn from a vein in your arm and Clotted sample is required.
Test Preparation Needed?
No Need any Preparation for this test.
What is being tested?
Hepatitis B is an infection of the liver caused by the hepatitis B virus (HBV). Hepatitis B blood tests detect viral proteins (antigens), the antibodies that are produced in response to an infection, or detect or evaluate the genetic material (DNA) of the virus.
There is Three Methods to detect HBV.
HbsAg ICT (Screening) MethodICT (Immuno chromatographic TEST) is a initial test method its called Screening Method.
- Patient’s Sample
- Test Tubes
- ICT Device
- Yellow Tips
- Patient’s Performa for Report
Reagent PreprationICT Device is ready to use and it is room temperature item.
- Open the ICT Device and mark it as pt. name or number
- add 100ul Patient’s Serum.Plasma
- Wait for 5-10 mints
- Read The Result
Some ICT Device companies methods are different. That is why read the manual carefully.
See the below chart
HbsAg ELISA Method
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.
Test RequirementsThere are Basic Requirements for this Technique.
Contents of the test kit:
All Reagents and samples temperature must 37°CWash Buffer:
The wash buffer is a 10x concentrate (1 part reagent plus 9 parts distilled water).
This Procedure is same of 80% of Different Companies kits.Cut-off: NC abs + 0.5 (some companies are different) NC abs = 0.127 Cut-off = NC abs + 0.5 = 0.127 + 0.5 = 0.627 Patient Result: Pt abs = 0.243 = Non Reactive Pt abs = 0.634 = Borderline Pt abs = 0.843 = Reactive
HBV DNA by PCR
Comming This Method