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Also Known As:  HBV TestsHep Banti-HBsHepatitis B Surface AntibodyHBsAgHepatitis B Surface AntigenHBeAg,, Hepatitis B e Antigenanti-HBcHepatitis B Core Antibodyanti-HBc, IgManti-HBeHepatitis B e AntibodyHBV DNA ,HBV PCR

Why Get Tested?

Primarily to screen for and diagnose acute or chronic hepatitis B virus (HBV) infection, to detect a previous, resolved hepatitis B infection, or sometimes to guide and monitor treatment

When To Get Tested?

When you have risk factors for HBV infection or when you have signs and symptoms of hepatitis, such as jaundice or unexplained elevated blood levels of alanine aminotransferase (ALT), a liver-associated enzyme; when you have a condition that requires chemotherapy or drugs that suppress your immune system; when you are being treated for HBV or hepatitis C (HCV); when it is unclear whether you have immunity and your healthcare practitioner is considering giving you the hepatitis B vaccineing you the hepatitis B vaccine.

Sample Required?

A blood sample drawn from a vein in your arm and Clotted sample is required.

Test Preparation Needed?

No Need any Preparation for this test.

What is being tested?

Hepatitis B is an infection of the liver caused by the hepatitis B virus (HBV). Hepatitis B blood tests detect viral proteins (antigens), the antibodies that are produced in response to an infection, or detect or evaluate the genetic material (DNA) of the virus.

Test Procedure

There is Three Methods to detect HBV.

  1. HbsAg ICT (Screening) Method
  2. HbsAg Elisa Method

HbsAg ICT (Screening) Method

ICT (Immuno chromatographic TEST) is a initial test method its called Screening Method.

Test Requirements

Some Laboratories use Dropper and they not use micropipettes.

Reagent Prepration

ICT Device is ready to use and it is room temperature item.

Test Procedure

  • Open the ICT Device and mark it as pt. name or number
  • add 100ul Patient’s Serum.Plasma
  • Wait for 5-10 mints
  • Read The Result

Some ICT Device companies methods are different. That is why read the manual carefully.

Result Preprations

See the below chart

HbsAg ELISA Method

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.


Test Requirements

There are Basic Requirements for this Technique.
  • Patient’s Sample
  • Incubator
  • Centrifuge
  • Micropipettes (10ul – 100ul & 100ul – 1000ul)
  • Microplate Washer or Manually Washing Bottles
  • Test Kits (EIA)
  • Plate Reader
  • Patient’s Performa for Report
  • Contents of the test kit:

  • Microplate wells (12×8)
  • Calibrator (1×2.0ml)
  • Positive control (1×2.0ml)
  • Negativecontrol (1×2.0ml)
  • Enzyme conjugate (1x12ml)
  • Wash buffer (1x100ml)
  • Chromogen/substrate solution (1x12ml)
  • Stop solution (1x12ml)
  • Test Instruction (1 booklet)
  • Reagent Prepration

    All Reagents and samples temperature must 37°C

    Wash Buffer:

    The wash buffer is a 10x concentrate (1 part reagent plus 9 parts distilled water).

    Test Procedure

  • Add 150ul Standards, NC, PC and Sample in each well/li>
  • Incubate 30 mint at 37&#176C
  • Wash Wells 3 times with 400ul Wash Buffer(See Reagents Preparation)
  • add 100ul Conjugate in each Wells
  • Incubate 30 mint at 37&#176C
  • Wash Wells 3 times with 400ul Wash Buffer(See Reagents Preparation)
  • Add 100ul Substrate/TMB/chromogen Solution in each well
  • Incubate 20 mint at room temperature
  • Add 50 Micro Stop Solution in each well
  • colour intensity should be made at a wavelength of 450nm and a reference wavelength of between 620nm and 650nm within 30 minutes of adding the stop solution.
  • Result Preprations

    This Procedure is same of 80% of Different Companies kits.

    NC abs + 0.5 (some companies are different)
    NC abs = 0.127
    Cut-off = NC abs + 0.5
    = 0.127 + 0.5 = 0.627
    Patient Result:
    Pt abs = 0.243 = Non Reactive
    Pt abs = 0.634 = Borderline
    Pt abs = 0.843 = Reactive

    HBV DNA by PCR

    Comming This Method

    Possible References Used

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