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Sugar Test Method

A blood sugar test is a procedure that measures the amount of sugar, or glucose, in your blood. Your doctor may order this test to help diagnose diabetes.

Also Known as: Fasting Blood Sugar, FBS, Fasting Blood Glucose, Blood Sugar Fasting, BSF, BSL

Test Panel: Blood SugarFasting Blood SugarFBSFasting Blood GlucoseFBGFasting Plasma GlucoseFPGBlood GlucoseOral Glucose Tolerance TestOGTTGTTUrine Glucose

Blood Sugar Test
Fasting Blood Sugar

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Principle:

Glucose is oxidised to gluconic acid and hydrogen peroxide in the presence of glucose oxidase. Hydrogen peroxide further reacts with phenol and 4 – aminoantipyrine by the catalytic action of peroxidase to form a red coloured quinoneimine dye complex. Intensity of the colour formed is directly proportional to the amount of glucose present in the sample.

Glucose Test Principle

Normal Values:

  • Fasting Blood Sugar: 74 to 106 mg/dL (4.5 to 5.9 mmol/L)
  • Random Blood Sugar: 80 to 120 mg/dl (4.4 to 6.6 mmol/L).

Kit Content:

L1 : Glucose Reagent2 x 150 ml, 3 x 150 ml, 1 x 1000 ml
S : Glucose Standard (100 mg/di)5.0 ml

Reagent Storage I stability

Contents are stable at 2-8°C till the expiry mentioned on the labels. Upon storage the Glucose reagent may develop a slight pink colour. This does not affect the performance of the test.

Reagent Preparation:

Reagents are ready to use.

Sample Required:

Serum, plasma, CSF. Glucose is reported to be stable in the sample for 7 days when stored at 2-8°C.

System Parameters:

ReactionEnd Point
Wavelength / filter505 nm (Hg 546 nm)/ Green
Zero SettingReagent Blank
lncub. Temperature37°C / R. T.
lncub. Time10 min. / 30 min.
Light path1cm
Delay Time
Read Time
No. of read.
Interval
Volume400 μL
Standard100 mg/di
Factor
React. SlopeIncreasing
Linearity500 mg/dl
Unitsmg/dl

Procedure:

Pipette into clean dry test tubes labelled as Blank (B), Standard (S), and Test (T):

Additiion SequenceB (Blank)S (Standard)T (Test)
Glucose Reagent (L1)1000 μL1000 μL1000 μL
Distilled water010 μL
Glucose Standard (S)10 μL
Sample10 μL

Mix well and incubate at 37°C for 10 min. or at R.T. (25°C) for 30 min. Measure the absorbance of the Standard (Abs.S), and Test Sample (Abs.T) against the Blank, within 60 Min.

Test Calculation:

Glucose Test Calculation

Linearity:

This procedure is linear upto 500 mg/di. If values exceed this limit, dilute the serum with normal saline (NaCl 0.9%) and repeat the assay. Calculate the value using the proper dilution factor.

Related Articles:



Possible References Used


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