Leishman stain, also known as Leishman’s stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas.
Leishman Stain (Stock Solution)
Microscopic Glass Slide
Phosphate buffer (pH 6.8)
Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas. It is based on a methanolic mixture of “polychromed” methylene blue. (i.e. demethylated into various azures) and eosin.The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step.
Leishman stain – 0.150 gm
Methanol, absolute – 100.000 ml
Use smears that are as thin as possible and air-dried.
Fully cover the smears with Leishman’s Stain solution. Stain for 2 minuted.
Add twice the amount of distilled water and mix by swirling. Incubate for at least 10 min.