Test Procedure:There are two methods are explained
ICT MethodICT (Immuno chromatographic TEST) is a initial test method its called Screening Method.
- Patient’s Sample
- Test Tubes
- ICT Device
- ICT Device Buffer
- Yellow Tips
- Patient’s Performa for Report
Reagent PreprationICT Device Buffer is ready to use and it is room temperature item.
- Open the ICT Device and mark it as pt. name or number
- add 10ul Patient’s Serum.Plasma
- Add 2 drops ICT Device Buffer
- Wait for 5-10 mints
- Read The Result
Some ICT Device companies methods are different. That is why read the manual carefully.
See the below chart
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.
Test RequirementsThere are Basic Requirements for this Technique.
Contents of the test kit:
All Reagents and samples temperature must 37°CPatient’s Sample:
The patient samples for analysis are diluted 1:101 with sample buffer. For example, add 10 µl serum to 1.0 ml sample buffer and mix well. Incubate the mixture for at least 10 minutes at room temperature.Wash Buffer:
The wash buffer is a 10x concentrate (1 part reagent plus 9 parts distilled water).
This Procedure is same of 80% of Different Companies kits.Cut-off: Standare Value = 0.5 (some companies are different) Std abs = 0.127 Cut-off = std abs + std value = 0.127 + 0.5 = 0.627 Patient Result: Pt abs = 0.243 = Non Reactive Pt abs = 0.634 = Borderline Pt abs = 0.843 = Reactive