Microbiology MCQs with Answer and Explnation | Chapter 1

Answer: Aspergillus

The most serious fungal threat in a bone marrow transplant unit is Aspergillus. Aspergillus is a common fungus that can cause a serious infection called aspergillosis in people with weakened immune systems. Bone marrow transplant recipients are particularly susceptible to aspergillosis because their immune systems are suppressed to prevent rejection of the transplanted marrow. Aspergillosis can cause a variety of symptoms, including fever, cough, shortness of breath, and chest pain. It can also spread to other parts of the body, including the brain, and can be fatal.

• Candida albicans is another common fungus that can cause infections in people with weakened immune systems. However, Candida infections are generally less serious than aspergillosis and are more easily treated. Blastomyces and Cryptococcus are also fungi that can cause infections in people with weakened immune systems, but they are less common than Aspergillus and Candida.

Answer: N. Meningitidis

The Quellung reaction is a test used to identify bacteria by visualizing their capsules. In this test, specific antibodies are added to a suspension of bacteria. If the bacteria have the corresponding capsule, the antibodies will bind to it and cause it to swell. This swelling can be seen under a microscope as a halo around the bacteria.

• Haemophilus influenzae, Salmonella typhi, and Streptococcus pneumoniae all have capsules and will give a positive Quellung reaction.
• Neisseria meningitidis does not have a capsule and will give a negative Quellung reaction.

Answer: John Tyndall

John Tyndall, an Irish physicist, conducted experiments in the 1860s that demonstrated that open tubes of broth remained free of bacteria when air was free of dust. He showed that dust particles carried microorganisms and that if the air was filtered to remove dust, the broth remained sterile. This was a significant contribution to the understanding of spontaneous generation, the disproven theory that living organisms could arise from non-living matter.

• Abbc Spallanzani, an Italian biologist, also conducted experiments in the 18th century that challenged spontaneous generation, but his work did not specifically focus on the role of dust in bacterial contamination.
• Francisco Redi, an Italian physician, conducted experiments in the 17th century that disproved spontaneous generation in maggots, but his work did not involve broth or dust.
• Louis Pasteur, a French chemist and microbiologist, is famous for his experiments that окончательно disproved spontaneous generation in the 19th century. He built upon Tyndall’s work and showed that microorganisms could not grow in sterile broth even when exposed to unfiltered air, as long as the broth was not contaminated.

Answer: Zymase

The enzymes responsible for alcoholic fermentation are typically referred to as “Zymase.” Zymase is a complex of enzymes found in yeast cells that catalyze the conversion of sugars, such as glucose, into alcohol (ethanol) and carbon dioxide during the process of alcoholic fermentation. Ketolase, peroxidase, and oxidase are enzymes with different functions and are not directly involved in alcoholic fermentation.

Answer: Luciferase

The light emitted by luminescent bacteria is mediated by the enzyme luciferase. Luciferase catalyzes a chemical reaction that converts chemical energy into light energy. The specific reaction varies depending on the species of luminescent bacteria, but it typically involves the oxidation of a molecule called luciferin. This reaction releases energy in the form of light, which is what we see as bioluminescence.

Coenzyme Q, lactose dehydrogenase, and carboxylase reductase are not involved in bioluminescence. Coenzyme Q is a molecule that is involved in cellular respiration. Lactose dehydrogenase is an enzyme that breaks down lactose, a sugar found in milk. Carboxylase reductase is an enzyme that is involved in the synthesis of fatty acids.

Answer: Q-fever

Q-fever is diagnosed by serologic means. Serologic tests are used to detect antibodies in the patient’s blood, which can confirm exposure to Coxiella burnetii, the bacterium responsible for Q-fever. This method is particularly useful in diagnosing chronic or past infections as well as recent ones. The other conditions, such as actinomycosis, pulmonary tuberculosis, and gonorrhea, are typically diagnosed using other methods, such as culturing bacteria or detecting their DNA.

Answer: Conjugation

Conjugation is a mechanism of horizontal gene transfer in bacteria. It involves the direct transfer of genetic material, typically in the form of plasmids, from one bacterium to another through a structure called a sex pilus. This process allows for the exchange of genetic information, including antibiotic resistance genes and other beneficial traits, between bacterial cells. Transposition, translocation, and transduction are different mechanisms of genetic transfer in bacteria, each with its own distinct processes and features.

Answer: Malaise

The symptom “general feeling of illness and discomfort” is commonly referred to as Malaise. Malaise is a non-specific term used to describe a sense of unease, discomfort, or a general feeling of being unwell without clearly defined symptoms.

Incorrect Options:

1. Anaphylactic Shock: Anaphylactic shock is a severe and potentially life-threatening allergic reaction characterized by a rapid onset of symptoms, including difficulty breathing, low blood pressure, and other systemic reactions. It is not synonymous with a general feeling of illness and discomfort.
2. Arthritis: Arthritis refers to inflammation of the joints, leading to pain, swelling, and stiffness. While arthritis can cause discomfort, it is a specific condition related to joint inflammation, not a general feeling of illness.
3. Cystitis: Cystitis is the inflammation of the bladder, usually caused by a urinary tract infection. Symptoms include pain and a burning sensation during urination, frequent urination, and lower abdominal discomfort. It is not commonly associated with a general feeling of illness.

Answer: Alfred D.Hershey & Martha Chase in 1952

Hershey and Chase conducted a series of experiments in 1952 that provided definitive evidence that bacteriophage nucleic acid, not protein, enters the host cell during infection. Their experiments involved using radioactive isotopes to label the DNA and protein components of bacteriophages, which are viruses that infect bacteria. They then allowed the bacteriophages to infect bacteria and tracked the radioactive isotopes to determine which component entered the host cell. They found that only the radioactively labeled DNA entered the host cell, while the radioactively labeled protein remained outside. This experiment was a landmark in the history of biology, as it provided strong evidence that DNA is the genetic material.

The other options are not correct. Alfred D. Hershey and Leonard Tatum did not collaborate on research related to bacteriophages. Alfred D. Hershey and Zindar Lederberg did not conduct experiments on bacteriophages. Alfred D. Hershey and Macleod did not collaborate on research related to bacteriophages.

Answer: Ribosomes

Ribosomes are the least likely to contain useful antigens among the options provided. Antigens are typically found on the surface or in the outer structures of bacteria. Ribosomes are intracellular organelles involved in protein synthesis and are not typically exposed to the host immune system, so they are less likely to be involved in an immune response. In contrast, the cell wall, flagella, and capsule are more likely to contain antigens that can stimulate an immune response because they are exposed to the external environment or the host’s immune system.

Answer: Streptococcus Pyogenes

Streptococcus pyogenes, also known as Group A Streptococcus (GAS), is a bacterial pathogen responsible for various infections, including strep throat and other respiratory infections. When a throat culture is performed, the objective is to identify the presence of this bacterium, as it is a common cause of throat infections. The other bacteria mentioned, Staphylococcus epidermidis, Neisseria meningitidis, and Pseudomonas aeruginosa, are not typically the primary targets of a throat culture and are associated with different types of infections.

Answer: Log Phase

During the Log Phase, bacteria experience rapid exponential growth, where they divide and reproduce at their maximum rate. This phase is characterized by high metabolic activity, as the bacterial population is actively synthesizing DNA, proteins, and other cellular components. Nutrient utilization is at its peak, and the bacteria are producing energy and biomass at a high rate. The other phases mentioned – Lag Phase, Stationary Phase, and Death Phase – have different characteristics and levels of metabolic activity.

Answer: Produce a hypertonic environment

Salt and sugar preserve foods by creating a hypertonic environment, which means that the concentration of salt or sugar outside the cells of microorganisms is higher than the concentration inside the cells. This causes water to move out of the cells of the microorganisms, which dehydrates them and prevents them from growing or reproducing.

The other options are not correct. Making food acidic can also help to preserve it, but this is not the primary mechanism by which salt and sugar preserve food. Producing a hypotonic environment would actually encourage the growth of microorganisms, as it would cause water to move into their cells. Depleting nutrients would also not be effective in preserving food, as microorganisms can still grow and reproduce even in the absence of nutrients.

Answer: Mycoplasma

The bacterial genus where sterols are present in the cell membrane is Mycoplasma. Mycoplasmas are a group of bacteria that lack a cell wall. They are the smallest known bacteria, and they are able to survive in a variety of environments.

Mycoplasma cells are surrounded by a cell membrane that contains sterols. Sterols are molecules that help to stabilize the cell membrane and make it more resistant to osmotic shock.

The other bacterial genera listed in the question, Vibrio, Escherichia, and Chlamydia, all have cell walls and do not contain sterols in their cell membranes.

Answer: Lipopolysaccharides

Endotoxins are a type of toxic molecule associated with the outer membrane of gram-negative bacteria, and they are primarily composed of lipopolysaccharides (LPS). LPS is a complex molecule consisting of lipids and carbohydrates and is a key component of the outer membrane of these bacteria.

Answer: Staphylococcus aureus from Staphylococcus epidermidis

The coagulase test is done to differentiate Staphylococcus aureus from other species of Staphylococcus, such as Staphylococcus epidermidis. Coagulase is an enzyme that clots plasma. Staphylococcus aureus produces coagulase, while Staphylococcus epidermidis does not.

Therefore, the correct answer is: Staphylococcus aureus from Staphylococcus epidermidis.

The other options are incorrect:

• Staphylococcus epidermidis and Neisseria meningitidis are two different genera of bacteria. Neisseria meningitidis is a Gram-negative bacterium, while Staphylococcus epidermidis is a Gram-positive bacterium.
• Streptococcus pyogenes and Enterococcus faecalis are both Gram-positive cocci, but they can be differentiated by other tests, such as the catalase test and the bile esculin test.
• Streptococcus pyogenes and Staphylococcus aureus can both be differentiated by the coagulase test.

Answer: Leeuwenhoek

The words you quoted are from Anton van Leeuwenhoek, a Dutch scientist who is considered to be the father of microbiology. He was the first person to observe and describe microorganisms using a microscope.

In the passage you quoted, Leeuwenhoek is describing what he saw when he looked at a sample of rainwater under his microscope. He saw tiny green filaments that were moving in a spiral pattern. He was the first person to see bacteria, and his observations helped to establish the germ theory of disease.

The other scientists you listed also made important contributions to microbiology, but they were not the ones who first observed bacteria.

• Edward Jenner was an English physician who developed the first vaccine for smallpox.
• Louis Pasteur was a French chemist and microbiologist who made many important contributions to microbiology, including the discovery of the germ theory of disease and the development of pasteurization.
• Robert Koch was a German physician and microbiologist who discovered the causative agents of tuberculosis and cholera.

Answer: Staphylococcus Epidermidis

Staphylococcus epidermidis is a common skin commensal bacterium that is part of the normal flora on the skin’s surface. When the venipuncture site is not properly disinfected, bacteria from the skin, like S. epidermidis, can enter the bloodstream and contaminate the blood culture. While Staphylococcus aureus and Streptococcus pyogenes can cause infections, they are less commonly contaminants in blood cultures due to their more pathogenic nature. Escherichia coli is a gut bacterium and would not typically be a contaminant from inadequate skin disinfection during venipuncture.

Answer: Actinomyces and Clostridium

Actinomyces are bacteria that are found in the soil and in the mouths of humans and animals. They can cause a variety of infections, including actinomycosis, which is a rare but serious infection that can affect the lungs, brain, and other organs.

Clostridium are bacteria that are found in the soil and in the intestines of humans and animals. Some strains of Clostridium can cause food poisoning, such as botulism and tetanus. Others can cause diarrhea, such as Clostridium difficile-associated diarrhea.

Rhizobium are bacteria that are found in the soil and in the roots of legumes. They form a symbiotic relationship with legumes, helping them to fix nitrogen from the air.

Answer: Neuberg chamber

Direct microscopic count can be done with the aid of a Neuberg chamber. A Neuberg chamber, also known as a hemocytometer, is a specialized slide used for counting cells under a microscope. It has a grid of etched lines that divide the slide into small squares of known volume. By counting the number of cells in a specific number of squares, the total number of cells in the sample can be estimated.

• Anaerobic chambers, mineral oil, and olive oil are not used for direct microscopic counts. Anaerobic chambers are used to create an oxygen-free environment for culturing anaerobic bacteria. Mineral oil and olive oil are sometimes used to prevent evaporation of the sample during microscopic examination, but they are not used for direct counting.

Answer: All of the above

Nitrogen fixation is the process of converting atmospheric nitrogen (N2) into a form that can be used by plants and other organisms. This process can occur through both biological and non-biological means.

• Biological nitrogen fixation is carried out by certain bacteria, called diazotrophs, that have the ability to convert N2 into ammonia (NH3). This process is essential for life on Earth, as it is the primary source of nitrogen for plants.
• Non-biological nitrogen fixation can occur through a variety of processes, including lightning, ultraviolet light, and combustion. These processes convert N2 into nitrogen oxides (NOx), which can then be converted into ammonia by other bacteria.

Answer: Streptococcus pyogenes stains blue because of a thick peptidoglycan layer

In Gram staining, Streptococcus pyogenes would actually stain purple (violet), not blue. This is because it is a Gram-positive bacterium, and the thick peptidoglycan layer in the cell wall retains the crystal violet stain, giving it a purple color. The other statements are correct: Mycobacterium tuberculosis has a thick lipid layer and stains red (not blue), Escherichia coli has a thin peptidoglycan layer and stains pink, and Mycoplasma pneumoniae is not visible in the Gram’s stain because it lacks a typical cell wall.

Answer: Bdellovibrio

The bacterium that infects other gram-negative bacteria is Bdellovibrio. Bdellovibrio is a genus of predatory bacteria that attack and kill other gram-negative bacteria. Bdellovibrio cells are very small and can penetrate the cell walls of their prey bacteria. Once inside the prey cell, Bdellovibrio cells multiply and consume the prey cell’s cytoplasm.

The other bacteria listed in the question, Proteus mirabilis, Haemophilus influenzae, and Pseudomonas putida, are all gram-negative bacteria that can cause human infections, but they are not predatory bacteria.

Bdellovibrio bacteria are being studied as potential new treatments for human bacterial infections. Bdellovibrio bacteria are naturally occurring in the environment and are not known to cause any harm to humans.

Answer: Chlorophyll a

Chlorophyll a is a green pigment that absorbs light in the blue and red regions of the visible spectrum. It is the most important pigment in photosynthesis, the process by which plants use sunlight to convert carbon dioxide and water into oxygen and sugar.

• Chlorophyll b is a similar pigment to chlorophyll a, but it absorbs light in the green and yellow regions of the spectrum. It is less efficient at absorbing sunlight than chlorophyll a, but it still plays an important role in photosynthesis.
• Porphyrin is a ring-shaped molecule that is found in chlorophyll a and chlorophyll b. It is also found in other biological molecules, such as hemoglobin, which carries oxygen in the blood.
• Rhodopsin is a pigment that is found in the retina of the eye. It is responsible for vision in dim light.

Answer: Contains proteins and phospholipids

Prokaryotic cell membranes are composed of a phospholipid bilayer that is selectively permeable, meaning it allows certain molecules to pass through while blocking others. Additionally, the cell membrane contains various proteins that serve important functions, including transport, signal transduction, and structural support. The membrane also plays a role in regulating the entry and exit of materials. While some metabolic enzymes may be associated with the cell membrane, they are not a defining characteristic of the membrane itself.

Answer: a patient with leukemia

Reverse isolation is a type of isolation used to protect patients with weakened immune systems from infection. Patients with leukemia, a type of cancer that affects the blood and bone marrow, often have weakened immune systems due to their disease and treatment. This makes them more susceptible to infections, which can be serious or even life-threatening.

Reverse isolation involves placing the patient in a private room and taking steps to minimize their exposure to germs. This may include requiring healthcare workers and visitors to wear masks, gloves, and gowns, and restricting the number of visitors allowed in the room.

The other options are not appropriate for reverse isolation. Patients with tuberculosis, a contagious lung infection, are typically placed in isolation to prevent the spread of the disease to others. Patients who have had minor surgery and patients with glaucoma, an eye disease, do not typically have weakened immune systems and do not require reverse isolation.

Answer: Cyanophyceae

Spirulina is a genus of cyanobacteria, which are photosynthetic bacteria that were once classified as algae. Cyanobacteria are often referred to as blue-green algae because of their characteristic color. They are found in a wide variety of habitats, including freshwater, saltwater, and soil.

• Xanthophyceae, Rhodophyceae, and Pheophyceae are all classes of algae, but they are not closely related to cyanobacteria. Xanthophyceae are yellow-green algae, Rhodophyceae are red algae, and Pheophyceae are brown algae.

Answer: Ascospores

Spores are reproductive units produced by fungi. They can be produced either sexually or asexually.

• Asexual spores are produced by mitosis and are genetically identical to the parent fungus.
• Sexual spores are produced by meiosis and are genetically diverse.
• Ascospores are sexual spores that are produced in a sac-like structure called an ascus. They are found in Ascomycota, a phylum of fungi that includes yeasts, molds, and truffles.
• Conidia and sporangiospores are asexual spores. Conidia are produced by budding from the tip of a specialized hypha called a conidiophore. Sporangiospores are produced in a sac-like structure called a sporangium.

Answer: Within Periplasmic space

The periplasmic space is the region located between the bacterial cell membrane and the cell wall. β-Lactamases are enzymes that bacteria produce to break down and inactivate β-lactam antibiotics, which include penicillins and cephalosporins. These enzymes are typically found in the periplasmic space, where they can interact with and degrade antibiotics before they reach their target sites within the bacterial cell. This localization allows bacteria to resist the action of β-lactam antibiotics by producing β-lactamases in the periplasm. The other locations mentioned, such as within the capsule, free in the cytoplasm, or attached to the nucleoid, are not the primary sites for β-lactamase activity.

Answer: Killed Vaccine

The Pertussis Vaccine, also known as the whooping cough vaccine, contains inactivated or killed components of the bacterium Bordetella pertussis, which causes whooping cough. This killed vaccine stimulates an immune response against the pathogen without causing the disease itself. It is used to protect individuals, particularly infants and young children, from developing severe whooping cough. The other vaccine types mentioned – Cellular Fraction Vaccine, Toxoid Vaccine, and Live Attenuated Vaccine – are used for different purposes and work through different mechanisms.

Answer: Corynebacterium Diphtheriae

Metachromatic granules are also known as Babes-Ernst granules or volutin granules. These granules are found in certain bacteria, including Corynebacterium diphtheriae. When stained with certain dyes, metachromatic granules appear as distinct structures within the bacterial cells. They are a diagnostic feature used to identify Corynebacterium diphtheriae, which is the causative agent of diphtheria, a bacterial infection. The other bacteria listed, Neisseria gonorrhoeae, Streptococcus pyogenes, and Staphylococcus aureus, do not typically exhibit metachromatic granules as a prominent characteristic.

Answer: Enterococcus faecalis

Enterococcus faecalis is a recognized cause of diarrhea, but it is less common than the other options listed. It is typically associated with antibiotic-associated diarrhea and healthcare-associated infections.

Vibrio cholerae, Escherichia coli, and Clostridium perfringens are all common causes of diarrhea.

• Vibrio cholerae is the causative agent of cholera, a severe diarrheal disease that can be fatal if it is not treated.
• Escherichia coli is a common bacterium that lives in the intestines of humans and animals. Some strains of E. coli can cause diarrhea, including traveler’s diarrhea and food poisoning.
• Clostridium perfringens is a bacterium that can be found in soil and food. It can cause food poisoning, which is often characterized by diarrhea, abdominal cramps, and nausea.

Answer: Griffith

Bacterial transformation was discovered by Frederick Griffith in 1928. Griffith’s experiment involved injecting mice with two different strains of Streptococcus pneumoniae bacteria: a virulent strain (S strain) that caused pneumonia and a non-virulent strain (R strain) that did not cause pneumonia. He found that when he injected mice with heat-killed S strain bacteria and live R strain bacteria, the mice died of pneumonia. This suggested that something from the dead S strain bacteria had transformed the live R strain bacteria into virulent bacteria.

In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty identified the transforming substance as DNA. They showed that when they purified DNA from the S strain bacteria and added it to R strain bacteria, the R strain bacteria were transformed into virulent bacteria. This was a landmark discovery in biology, as it provided definitive evidence that DNA is the genetic material.

Answer: Their cell wall is composed of peptidoglycan

The presence of a rigid cell wall composed of peptidoglycan in prokaryotic cells, such as those in bacteria, provides structural support and helps prevent the cell from bursting when exposed to changes in osmotic pressure. Eukaryotic cells lack such rigid cell walls and are more susceptible to osmotic shock when exposed to changes in solute concentrations.

Answer: Virtual

The image obtained in a compound microscope is virtual and inverted. This means that the image is not a real object, but rather a projection of the object that appears to be behind the microscope. The image is also inverted, meaning that it is upside down and flipped left to right compared to the object being viewed.

The reason for this is that the compound microscope uses two lenses to magnify the object: the objective lens and the eyepiece lens. The objective lens forms a real, inverted image of the object, which is then magnified by the eyepiece lens. The eyepiece lens then forms a virtual, inverted image of the real image, which is what the observer sees.

Answer: Quartz

The objective lens in a fluorescent microscope is typically made of quartz. Quartz is a type of glass that is transparent to ultraviolet (UV) light, which is used to excite the fluorophores in the sample. Glass, on the other hand, is not transparent to UV light, so it cannot be used for the objective lens in a fluorescent microscope. Polythene is not a suitable material for an objective lens because it is not transparent to visible light.

Answer: Immobilization

Immobilization is the process by which microorganisms convert elemental carbon into organic compounds. This process is an important part of the biogeochemical cycle of carbon, as it helps to store carbon in living organisms and in the soil.

• Dissimilation is the process by which microorganisms break down organic compounds into simpler molecules. This process is also important in the carbon cycle, as it releases carbon dioxide back into the atmosphere.
• Decomposition is the process by which dead organisms are broken down into simpler molecules. This process is also important in the carbon cycle, as it releases carbon dioxide back into the atmosphere.
• Neutralization is a chemical reaction that occurs when an acid and a base are mixed together. This process is not relevant to the carbon cycle.

Answer: Protease

The production of an exotoxin that inhibits the release of acetylcholine at the neuromuscular junction is characteristic of a Protease. Proteases are enzymes that can cleave proteins, and in this case, the exotoxin acts by interfering with the release of acetylcholine, a neurotransmitter responsible for transmitting nerve impulses to muscles. This interference can lead to muscle paralysis or weakness, which is a characteristic feature of certain bacterial infections, such as those caused by Clostridium botulinum, the bacterium responsible for botulism. Botulinum toxin, produced by C. botulinum, is an example of an exotoxin that acts as a protease and inhibits acetylcholine release at the neuromuscular junction.

Answer: Fermentation is a aerobic process

Louis Pasteur discovered that living organisms can distinguish between stereoisomers, which are molecules that have the same chemical formula but different spatial arrangements of their atoms. He found that yeast cells could ferment one enantiomer of a chiral molecule but not the other. This discovery was important because it helped to establish that living organisms are not simply chemical machines, but rather complex systems with the ability to recognize and respond to their environment.

Fermentation is an anaerobic process, meaning that it does not require oxygen.

Answer: IgM

IgM is the largest antibody molecule and is produced early in the immune response to an infection. It is also the most efficient antibody at activating the complement system, which is a group of proteins that helps to destroy invading pathogens.

• IgG is the most abundant antibody in the blood and is responsible for long-term immunity to infections. It is also the only antibody that can cross the placenta to protect a developing fetus from infection.
• IgA is found in secretions such as tears, saliva, and mucus, and helps to protect against infection at mucosal surfaces.
• IgD is found on the surface of B cells and helps to regulate B cell activation.

Answer: Z-N Stain

For the microscopic identification of Mycobacterium tuberculosis, the most commonly used stain is the Ziehl-Neelsen (Z-N) Stain, also known as the acid-fast stain. This stain is specifically designed to detect acid-fast bacteria, including M. tuberculosis, which retains the stain despite the application of acid or alcohol during the staining process. The other options mentioned, Giemsa stain, iodine preparation, and Gram stain, are used for different purposes and are not as effective in identifying M. tuberculosis.

Answer: E-Coli

Of the following options, only E. coli contains structures composed of N-acetylmuramic acid and N-acetylglucosamine. These two sugars are the building blocks of peptidoglycan, a polymer that forms the major component of the bacterial cell wall.

Mycoplasmas are bacteria that lack a cell wall, so they do not contain peptidoglycan. Amoebas are protozoa, not bacteria, and they also do not have peptidoglycan cell walls. Spheroplasts are bacteria that have had their cell walls partially or completely removed.

E. coli is a Gram-negative bacterium, and it has a thick peptidoglycan cell wall. The peptidoglycan layer gives E. coli its shape and helps to protect it from the environment.

Answer: Bradyrhizobium

Soybean plants form a symbiotic relationship with Bradyrhizobium bacteria. These bacteria live in nodules on the roots of soybean plants and fix nitrogen from the air into a form that the plants can use. This process is essential for soybean plants to grow and thrive.

The other options are not correct. Azatobactor paspali is a bacterium that forms a symbiotic relationship with grasses. Rhizobium is a genus of bacteria that forms symbiotic relationships with a variety of legumes, but not soybeans. Nostoc is a genus of cyanobacteria that can fix nitrogen, but it does not form symbiotic relationships with plants.

Answer: Bacteroides fragilis

Bacteroides fragilis is a Gram-negative anaerobic bacterium that is not typically responsible for urinary tract infections (UTIs). UTIs are most commonly caused by Gram-negative aerobic bacteria, such as Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae.

Bacteroides fragilis is more likely to cause UTIs in patients with structural abnormalities of the urinary tract, such as strictures or fistulas. It is also more likely to cause UTIs in patients who are immunocompromised or who have recently undergone surgery on the urinary tract.

Answer: Capsule

The capsule is a protective structure surrounding certain bacterial cells, and it is often involved in evading the host immune system. Capsules can exhibit significant antigenic variation, with different strains or species of bacteria having various types of capsules. This variation in capsule composition is an adaptation that allows bacteria to escape detection by the host’s immune system and contributes to their pathogenicity. The other options mentioned, such as peptidoglycan, spores, and Lipid A of endotoxin, do not typically exhibit as much antigenic variation as bacterial capsules.

Answer: Transport Media

It is designed to maintain the viability of clinical specimens containing enteric pathogens, such as Shigella, Salmonella, Escherichia coli, and other enteric bacteria during transportation to the laboratory for culture and analysis. Cary-Blair medium helps preserve the organisms and prevent their overgrowth or loss of viability during transit, making it a suitable transport medium for enteric pathogens. It is not typically used for differentiation, selectivity, or enrichment of specific microorganisms.

Answer: Excision and Integration of Plasmid

Transposase enzymes are involved in a process known as transposition, which is the movement of genetic material, often DNA segments, within a genome. In the context of plasmids, transposase enzymes facilitate the excision of a plasmid from one location and its integration into another. This allows for the transfer of genetic material, such as antibiotic resistance genes, from one part of the genome to another or between different organisms. Transposase enzymes play a crucial role in the mobility of genetic elements and are not directly responsible for transcription, the transfer of genes, or the transfer of resistance.

Answer: Peptidoglycan

Lysozyme is an enzyme found in tears, saliva, and other bodily fluids. It plays a role in the innate immune system by breaking down the peptidoglycan layer of bacterial cell walls. This action weakens the bacterial cell wall and can lead to bacterial cell lysis, which is an effective mechanism for preventing bacterial infections. It is important to note that Lysozyme does not target nucleoid DNA, plasmids, or pili directly.

Answer: Streptococcus pyogenes

Streptococcus pyogenes is not typically a cause of diarrhea. It is a gram-positive bacterium that is more commonly associated with infections such as strep throat, scarlet fever, and impetigo.

The other options listed are all common causes of diarrhea:

• Shigella dysenteriae is a gram-negative bacterium that causes shigellosis, also known as bacillary dysentery. It is a contagious disease that is characterized by severe diarrhea, fever, and abdominal cramps.
• Clostridium difficile is a gram-positive bacterium that causes antibiotic-associated diarrhea (AAD). AAD is a type of diarrhea that can occur after taking certain antibiotics, such as clindamycin and cephalosporins.
• Salmonella enteriditis is a gram-negative bacterium that causes salmonellosis, a type of food poisoning. Salmonella enteriditis is one of the most common causes of food poisoning in the United States.

Answer: Yeast artificial chromosomes

YACs are artificial chromosomes that can be propagated in yeast cells. They can carry large inserts of DNA, up to 1 million base pairs in length. This made them ideal for cloning large fragments of the human genome.

BACs are artificial chromosomes that can be propagated in bacteria. They can carry inserts of DNA up to 300,000 base pairs in length. BACs are more stable than YACs and are easier to work with, so they were also used extensively in the Human Genome Project.

Phagemid vectors, cosmid vectors, and yeast episomal plasmids are all vectors that can be used to clone DNA, but they are not able to carry inserts as large as YACs or BACs. Therefore, they were not used in the Human Genome Project.