The HiCN test method is a way to measure the amount of hemoglobin in your blood. Hemoglobin is the iron-rich protein in red blood cells that carries oxygen throughout your body.
Principle:
Whole blood is diluted 1 in 201 in a modified Drabkin’s solution which contains potassium ferricyanide and potassium cyanide. The red cells are haemolyzed and the haemoglobin is oxidized by the ferricyanide to methaemoglobin. This is converted by the cyanide to stable haemiglobincyanide (HiCN). Absorbance of the HiCN solution is read in a spectrophotometer at wavelength 540 nm or in a filter colorimeter using a yellow-green filter. The absorbance obtained is compared with that of a reference HiCN standard solution. Haemoglobin values are obtained from tables prepared from a calibration graph or if using a direct read-out haemoglobin meter, from the digital display
Test Requirements:
Micropipettes 10ul to 1000ul
2. Test Tubes,
3. Tissue or Cotton,.
4. HiCN Solution ( Drabkin’s solution )
5. Patient’s Blood
6. Colorimeter or Spectrophotometer
Test Procedure
Arrange The Test Tube in Racks.
2. Add 4ml HiCN Solution in Test Tube.
3. Add 20ul (0.02 ml) Patient’s Whole Blood.
4. Incubate at Room Temperature for 4-5 mint.
5. Set The Colorimeter wavelength 540nm at yellow-green filter.
6. Zero the colorimeter with Drabkin’s fluid and read the absorbance of the patient’s sample.
7. if Spectrophotometer then Read The Reading of Hb%. (Factor 35.5)
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