Investigation of possible acute interstitial nephritis
Eosinophils are white blood cells that normally do not appear in urine. The presence of eosinophils in the urine is seen in acute interstitial nephritis, which is caused by an allergic reaction, typically to drugs.
Container/Tube: Unstained slide
Specimen Volume: 1 slide
- Collect a random urine specimen.
- Spin 10 mL of a random urine collection in centrifuge.
- Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBCs are seen, the eosinophil test canâ€™t be performed. If WBCs are present, prepare slides using the spun sediment.
- Unstained slide should be prepared within 2 hours of collection.
- Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.
- Guidelines for slide preparation. Use the sediment to prepare slides as follows:
- 150 mcL of sample for 1 to 3 WBC/high power field (hpf).
- 100 mcL of sample for moderate to normal sediment (<50 cell/hpf).
- 50 mcL of sample for heavier sediment (>50 cells/hpf).
- 25 mcL for packed fields of sediment.
Greater than 5% eosinophils indicates acute interstitial nephritis; 1% to 5% eosinophils is indeterminant.
No significant cautionary statements.
1. Hansel FK: In Clinical Allergy. CV Mosby Co. St. Louis, 1953
2. Brunzel NA: Chapter 8: Microscopic Examination of Urine Sediment. In Fundamentals of Urine and Body Fluids Analysis. Third edition. Edited by NA Brunzel. Elsevier Saunders. St. Louis, MO, 2012, pp 171-172
Hansel stain.(Brunzel NA: Chapter 8: Microscopic Examination of Urine Sediment. In Fundamentals of Urine and Body Fluids Analysis. Third edition. Edited by NA Brunzel. Elsevier Saunders. St. Louis, MO, 2012, pp 171-172)
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