Sudan Black B Staining MCQs Mock Test (30 MCQs)

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Master Sudan Black B staining, a crucial histochemical technique for detecting lipids, myeloid granules, and leukocyte inclusions in hematopathology. This comprehensive guide covers:
✓ Principle of Sudan Black B binding to neutral fats & phospholipids
✓ Step-by-step protocol for blood smears and tissue sections
✓ Key applications in acute myeloid leukemia (AML) diagnosis
✓ Comparison with PAS and myeloperoxidase stains

Essential for hematologists, pathologists, and medical lab scientists working in oncology and metabolic disorder diagnostics.

Test your expertise with 25+ Sudan Black B MCQs! This mock exam covers:
◼️ Staining mechanism for myeloid lineage cells
◼️ Diagnostic patterns in AML vs. ALL
◼️ Troubleshooting false-positive results
◼️ Comparison with Oil Red O and Nile Blue stains

Ideal for hematology board exams, MLS certification, and oncology fellowship prep.

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Microbiology Department

Sudan Black B staining MCQs Mock Test

Essential for hematologists, pathologists, and medical lab scientists working in oncology and metabolic disorder diagnostics.

Ideal for hematology board exams, MLS certification, and oncology fellowship prep.

Read Full Article: Sudan Black B Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

What is the advantage of SBB over Myeloperoxidase (MPO) staining?

SBB is cheaper

SBB can stain older smears

SBB is water-soluble

SBB stains proteins more effectively

2 / 30

Category: Clinical Laboratory

What is the purpose of Nuclear Fast Red in SBB staining?

To fix the sample

To differentiate the stain

To counterstain nuclei

To stain lipids

3 / 30

Category: Clinical Laboratory

What should be done if SBB solution comes into contact with skin?

Ignore it

Cover with a bandage

Wash with soap and water

Apply alcohol

4 / 30

Category: Clinical Laboratory

Which cells are generally SBB-negative?

Myelocytic cells

Lymphoid cells

Monocytic cells

Basophils

5 / 30

Category: Clinical Laboratory

How should SBB powder dye be stored?

In a freezer

In water

In a tightly closed original package at 15-25°C

In direct sunlight

6 / 30

Category: Clinical Laboratory

What type of sample is required for SBB staining?

Saliva

Stool

Urine

Fresh anticoagulated whole blood or bone marrow smear

7 / 30

Category: Clinical Laboratory

What is the role of tap water in the staining process?

To enhance lipid staining

To dissolve the dye

To rinse slides after fixation and staining

To dehydrate the sample

8 / 30

Category: Clinical Laboratory

Which cells are typically SBB-positive?

Erythrocytes

Lymphoid cells

Myelocytic cells

Platelets

9 / 30

Category: Clinical Laboratory

What is the role of propylene glycol in SBB staining?

To fix the sample

To dissolve the dye and act as a differentiating agent

To enhance protein staining

To dehydrate the sample

10 / 30

Category: Clinical Laboratory

What color do nuclei appear after counterstaining with Nuclear Fast Red?

Black

Red

Blue

Green

11 / 30

Category: Clinical Laboratory

Which counterstain is commonly used with SBB?

May-Grunwald-Giemsa

Hematoxylin

Eosin

Crystal violet

12 / 30

Category: Clinical Laboratory

What does SBB positivity indicate in myelocytic cells?

Presence of carbohydrates

Presence of proteins

Presence of lipids in azurophilic and secondary granules

Presence of nucleic acids

13 / 30

Category: Clinical Laboratory

How should used SBB solutions be disposed of?

Dispose of as special waste

Reuse for other experiments

Burn in an open flame

Pour down the sink

14 / 30

Category: Clinical Laboratory

What PPE is recommended for SBB staining?

Sunglasses

Sandals

No PPE required

Gloves and lab coat

15 / 30

Category: Clinical Laboratory

What is the purpose of air-drying smears before staining?

To remove lipids

To ensure proper fixation and adherence

To enhance protein staining

To evaporate water

16 / 30

Category: Clinical Laboratory

What is the composition of the working SBB stain solution?

50 ml SBB solution + 50 ml buffer

60 ml SBB solution + 40 ml buffer

40 ml SBB solution + 60 ml buffer

70 ml SBB solution + 30 ml buffer

17 / 30

Category: Clinical Laboratory

What does a positive SBB reaction look like?

Black or granular pigment

Red nuclei

Blue granules

Green cytoplasm

18 / 30

Category: Clinical Laboratory

What is the primary solvent used in SBB staining?

Propylene glycol

Ethanol

Acetone

Water

19 / 30

Category: Clinical Laboratory

What is the stability of SBB dye solution?

6 months at room temperature

Indefinitely

1 year at 60°C

2 years at 4°C

20 / 30

Category: Clinical Laboratory

Which of the following is NOT a lipid stained by SBB?

Proteins

Sterols

Phospholipids

Neutral fats

21 / 30

Category: Clinical Laboratory

Which of the following is NOT a fixative used in SBB staining?

40% formaldehyde vapor

Acetic acid

Formalin-ethanol

Neutral buffered formalin

22 / 30

Category: Clinical Laboratory

What does blue-black staining indicate in SBB results?

Lipids

Nuclei

Carbohydrates

Proteins

23 / 30

Category: Clinical Laboratory

What is the primary use of SBB in hematology?

To differentiate AML from ALL

To detect viral infections

To measure hemoglobin levels

To identify bacterial infections

24 / 30

Category: Clinical Laboratory

What is Sudan Black B primarily used for?

Staining proteins

Differentiating AML from ALL

Detecting carbohydrates

Identifying viral infections

25 / 30

Category: Clinical Laboratory

How long should slides be fixed in formalin vapor for SBB staining?

10 minutes

1 minute

5 minutes

30 minutes

26 / 30

Category: Clinical Laboratory

What temperature should SBB solution be heated to for frozen sections?

100°C

37°C

60°C

120°C

27 / 30

Category: Clinical Laboratory

How long should slides be stained in SBB solution?

1 hour

2 hours

30 minutes

10 minutes

28 / 30

Category: Clinical Laboratory

What is the shelf life of SBB dye solution?

6 months

1 year

1 month

2 years

29 / 30

Category: Clinical Laboratory

Which of the following is NOT stained by Sudan Black B?

Sterols

Proteins

Phospholipids

Neutral fats

30 / 30

Category: Clinical Laboratory

What is the purpose of 70% alcohol in SBB staining?

To dehydrate the sample

To enhance lipid staining

To differentiate the stain by removing excess dye

To fix the sample

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The average score is 0%

Read Full Article: Sudan Black B Staining 50 FAQ and 30 MCQs

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