Wheatley Trichrome Staining MCQs Mock Test (30 MCQs)

Shares0Share0 Tweet0 Pin0 LinkedIn0 Email0

Test your parasitology skills with 30+ Wheatley’s Trichrome MCQs! This mock exam covers:
◼️ Staining mechanism for Giardia trophozoites & cysts
◼️ Troubleshooting faded stains
◼️ Differentiation of E. histolytica vs. E. coli
◼️ CDC-recommended protocols

Learn Wheatley’s Trichrome staining, the gold standard for intestinal parasite identification in stool samples. This guide covers:
✓ Principle behind trichrome staining for protozoa (Giardia, Entamoeba)
✓ Step-by-step protocol with modifications
✓ Color interpretation (cytoplasm vs. nuclear staining)
✓ Comparison with other stains (Iron-Hematoxylin, Iodine)

Essential for clinical microbiologists, parasitologists, and medical lab technicians diagnosing parasitic infections.

Ideal for MLT certification, ASCP exams, and tropical medicine courses.

0 votes, 0 avg

Microbiology Department

Wheatley Trichrome Staining MCQs Mock Test

Essential for clinical microbiologists, parasitologists, and medical lab technicians diagnosing parasitic infections.

Read Full Article: Wheatley Trichrome Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

What is the primary reason for using PVA in stool sample preparation?

To remove iodine

To preserve the stool sample

To stain the nuclei

To decolorize the smear

2 / 30

Category: Clinical Laboratory

What happens to glycogen molecules during staining?

They stain green

They stain red

They dissolve and appear clear

They stain purple

3 / 30

Category: Clinical Laboratory

What is the purpose of the acid ethanol step?

To fix the smear

To stain the nuclei

To remove iodine

To differentiate the stain

4 / 30

Category: Clinical Laboratory

What is the primary limitation of Wheatley Trichrome Stain for Cryptosporidium?

It requires acid-fast staining for Cryptosporidium

It over-stains Cryptosporidium

It cannot differentiate Cryptosporidium from yeast

It cannot stain Cryptosporidium

5 / 30

Category: Clinical Laboratory

What is the purpose of the ethanol rinse after decolorization?

To stain the nuclei

To halt decolorization

To remove iodine

To fix the smear

6 / 30

Category: Clinical Laboratory

How long should slides remain in Trichrome stain?

5 minutes

1 minute

10 minutes

30 minutes

7 / 30

Category: Clinical Laboratory

What color does the background stain in a Wheatley Trichrome smear?

Purple

Green

Blue

Red

8 / 30

Category: Clinical Laboratory

What is the purpose of xylene in the staining process?

To clear the slide

To stain the nuclei

To decolorize the smear

To fix the smear

9 / 30

Category: Clinical Laboratory

What color do human cells like RBCs and PMNs stain?

Blue

Green

Purple

Red

10 / 30

Category: Clinical Laboratory

What is the purpose of acid ethanol in the staining process?

To stain the nuclei

To remove iodine

To decolorize the smear

To fix the smear

11 / 30

Category: Clinical Laboratory

What is the primary purpose of the Wheatley Trichrome Stain?

Detecting intestinal protozoans

Staining helminth eggs

Staining bacterial cells

Identifying fungal spores

12 / 30

Category: Clinical Laboratory

Which protozoan is difficult to identify using Wheatley Trichrome Stain?

Cryptosporidium parvum

Giardia lamblia

Trichomonas vaginalis

Entamoeba histolytica

13 / 30

Category: Clinical Laboratory

What is the purpose of iodine-alcohol in the staining process?

To decolorize the smear

To fix the stool sample

To remove mercuric chloride

To stain the cytoplasm

14 / 30

Category: Clinical Laboratory

What is the primary limitation of Wheatley Trichrome Stain?

It cannot stain helminth eggs and larvae

It cannot stain yeast cells

It cannot stain protozoans

It cannot stain human cells

15 / 30

Category: Clinical Laboratory

What is the purpose of the iodine step in the staining process?

To decolorize the smear

To stain the nuclei

To remove mercuric chloride

To fix the stool sample

16 / 30

Category: Clinical Laboratory

What color do protozoan trophozoites stain?

Green

Yellow

Blue-green or light purple

Red

17 / 30

Category: Clinical Laboratory

What is the recommended drying time for a mounted smear?

Both a and c

Overnight at room temperature

1 hour at 37°C

2 hours at room temperature

18 / 30

Category: Clinical Laboratory

What magnification is used to examine the stained smear?

1000x

40x

400x

100x (oil immersion)

19 / 30

Category: Clinical Laboratory

What is the primary application of Wheatley Trichrome Stain?

Identifying fungal spores

Diagnosing intestinal protozoa infections

Staining bacterial cells

Staining helminth eggs

20 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of the Trichrome stain solution?

Phosphotungstic acid

Chromotrope 2R

Light Green SF

Methylene blue

21 / 30

Category: Clinical Laboratory

What is the recommended storage condition for Trichrome stain?

Room temperature, protected from light

Heated at 37°C

Refrigerated at 4°C

Frozen at -20°C

22 / 30

Category: Clinical Laboratory

What causes dark crystalline materials on the smear?

Saturation of iodine-alcohol solution

Over-decolorization

Over-staining

Inadequate drying

23 / 30

Category: Clinical Laboratory

Who originally developed the Trichrome stain?

Ziehl

Gomori

Neelsen

Wheatley

24 / 30

Category: Clinical Laboratory

What type of stool sample is used for Wheatley Trichrome staining?

Formalin-preserved only

Urine-contaminated stool

Frozen stool

Fresh or PVA-fixed

25 / 30

Category: Clinical Laboratory

Which of the following cannot be stained by Wheatley Trichrome Stain?

Human cells

Intestinal protozoan cysts

Yeast cells

Helminth eggs

26 / 30

Category: Clinical Laboratory

What modification did Wheatley make to Gomori’s Trichrome stain?

Eliminated the use of xylene

Added iodine-alcohol step

Added fixation and hydration steps

Reduced staining time to 1 minute

27 / 30

Category: Clinical Laboratory

What is the recommended fixative for stool samples in Wheatley Trichrome staining?

PVA or Schaudinn’s fixative

Xylene

Ethanol

Formalin

28 / 30

Category: Clinical Laboratory

What is the purpose of the mounting medium in the staining process?

To fix the smear

To preserve the stained smear

To remove iodine

To decolorize the smear

29 / 30

Category: Clinical Laboratory

What is the shelf life of the Trichrome stain solution?

6 months

24 months

36 months

12 months

30 / 30

Category: Clinical Laboratory

What is the primary purpose of the xylene step in the staining process?

To clear the slide

To fix the smear

To decolorize the smear

To stain the nuclei

Your score is

The average score is 0%

Read Full Article: Wheatley Trichrome Staining 50 FAQ and 30 MCQs

Possible References Used

⚠️ Disclaimer:

The content on LabTestsGuide.com is for informational and educational purposes only. We do not guarantee the accuracy, completeness, or timeliness of the information provided. Always consult qualified healthcare professionals for medical advice, diagnosis, or treatment. LabTestsGuide.com is not liable for any decisions made based on the information on this site.