Thioglycollate Broth MCQs Mock Test

Test your microbiology expertise with our Thioglycollate Broth Mock Test! This 30-question quiz challenges your knowledge of this essential enriched medium used for culturing aerobic, anaerobic, and microaerophilic organisms.

🔬 What You’ll Be Tested On:

✔ Medium Composition & Principles

Sodium thioglycollate (reducing agent)
Resazurin (oxygen indicator)
Nutrient components (casein, yeast extract)

✔ Clinical Applications

Blood culture isolation
Wound/pus specimen processing
Sterility testing (USP <71>)

✔ Interpretation Skills

Oxygen gradient patterns (aerobic vs anaerobic growth)
Resazurin color changes
Contamination identification

✔ Troubleshooting

Pink discoloration causes
Overgrowth issues
Incubation optimization

🎯 Perfect For:

Medical lab scientists (MLS/MLT certification prep)
Microbiology students (USMLE, ASCP exams)
Clinical microbiologists

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Thioglycollate Broth MCQs Mock Test

🔬 What You’ll Be Tested On:

✔ Medium Composition & Principles

Sodium thioglycollate (reducing agent)
Resazurin (oxygen indicator)
Nutrient components (casein, yeast extract)

✔ Clinical Applications

Blood culture isolation
Wound/pus specimen processing
Sterility testing (USP <71>)

✔ Interpretation Skills

Oxygen gradient patterns (aerobic vs anaerobic growth)
Resazurin color changes
Contamination identification

✔ Troubleshooting

Pink discoloration causes
Overgrowth issues
Incubation optimization

🎯 Perfect For:

Medical lab scientists (MLS/MLT certification prep)
Microbiology students (USMLE, ASCP exams)
Clinical microbiologists

Read Full Article: Thioglycollate Broth FAQs and MCQs

1 / 30

Category: Clinical Laboratory

Which bacteria would grow at the top of the tube?

Bacteroides vulgatus

Clostridium perfringens

Micrococcus luteus

Peptostreptococcus anaerobius

2 / 30

Category: Clinical Laboratory

Reheating Thioglycollate Broth multiple times can lead to:

Increased nutrient levels

Longer shelf life

Toxic oxygen radicals

Better anaerobiosis

3 / 30

Category: Clinical Laboratory

Which of the following is a limitation of Thioglycollate Broth?

Cannot support fastidious bacteria

Must be freshly prepared or reheated before use

Only grows aerobic bacteria

Turns blue when sterile

4 / 30

Category: Clinical Laboratory

Which ingredient in Thioglycollate Broth acts as a reducing agent?

Yeast extract

Sodium thioglycollate

Glucose

Agar

5 / 30

Category: Clinical Laboratory

Which regulatory body does NOT reference Thioglycollate Broth?

CDC

European Pharmacopeia

APHA

USP

6 / 30

Category: Clinical Laboratory

Obligate anaerobes grow predominantly in which zone?

Bottom

Surface

Uniformly

Middle

7 / 30

Category: Clinical Laboratory

Why should Thioglycollate Broth not be stored at low temperatures?

Increases oxygen absorption

Kills bacteria

It solidifies

Changes color

8 / 30

Category: Clinical Laboratory

What is the correct incubation temperature for Thioglycollate Broth?

30-35°C

4°C

42°C

25°C

9 / 30

Category: Clinical Laboratory

Which standard recommends Thioglycollate Broth for sterility tests?

USP (United States Pharmacopeia)

CDC

WHO

FDA

10 / 30

Category: Clinical Laboratory

What is the role of L-cystine in Thioglycollate Broth?

Neutralizes peroxides and supports anaerobes

Inhibits Gram-negative bacteria

Provides carbon source

Acts as a pH indicator

11 / 30

Category: Clinical Laboratory

How should Thioglycollate Broth be stored?

Frozen

Upright, sealed, and protected from light

With loose caps

In direct sunlight

12 / 30

Category: Clinical Laboratory

What is the primary purpose of Thioglycollate Broth?

To differentiate bacteria based on sugar fermentation

To inhibit fungal growth

To selectively grow Gram-positive bacteria

To determine oxygen requirements of microorganisms

13 / 30

Category: Clinical Laboratory

What does flocculent growth in Thioglycollate Broth indicate?

No growth

Viral infection

Fungal/yeast growth (e.g., Candida)

Bacterial contamination

14 / 30

Category: Clinical Laboratory

Which organism grows as “puffballs” in Thioglycollate Broth?

Pseudomonas aeruginosa

Bacteroides fragilis

E. coli

Streptococcus pneumoniae

15 / 30

Category: Clinical Laboratory

How is Thioglycollate Broth sterilized?

UV radiation

Filtration

Boiling for 5 minutes

Autoclaving at 121°C for 15 minutes

16 / 30

Category: Clinical Laboratory

What should be done if the medium turns pink before use?

Discard it

Adjust pH with HCl

Add more glucose

Reheat it to remove oxygen

17 / 30

Category: Clinical Laboratory

Why is agar (0.075%) sometimes added to Thioglycollate Broth?

To solidify the medium

To prevent convection currents

To inhibit anaerobes

To enhance bacterial motility

18 / 30

Category: Clinical Laboratory

What is the purpose of the pancreatic digest of casein?

To change color with pH

To act as a reducing agent

To inhibit fungi

To provide nitrogen and amino acids

19 / 30

Category: Clinical Laboratory

If the medium is pink throughout, it indicates:

pH imbalance

Complete oxidation (unsuitable for anaerobes)

Bacterial growth

Proper anaerobic conditions

20 / 30

Category: Clinical Laboratory

Thioglycollate Broth is used for sterility testing of:

All of the above

Antibiotics

Food samples

Blood cultures

21 / 30

Category: Clinical Laboratory

Why is sodium thioglycollate added to the medium?

To increase pH

To solidify the broth

To neutralize heavy metals

To inhibit Gram-positives

22 / 30

Category: Clinical Laboratory

Facultative anaerobes show growth:

Only at the bottom

Only at the top

Throughout, but denser at the top

In a single colony

23 / 30

Category: Clinical Laboratory

What does resazurin indicate in Thioglycollate Broth?

pH change

Protein digestion

Oxygen presence (pink = oxidized)

Glucose fermentation

24 / 30

Category: Clinical Laboratory

Why is blood diluted 1:10 in Thioglycollate Broth for cultures?

To inhibit bacteria

To enhance oxygen levels

To prevent clotting

To reduce turbidity

25 / 30

Category: Clinical Laboratory

Which organism is commonly tested in QC for Thioglycollate Broth?

Escherichia coli

Clostridium sporogenes

Pseudomonas fluorescens

Staphylococcus epidermidis

26 / 30

Category: Clinical Laboratory

Where do obligate aerobes grow in Thioglycollate Broth?

Top

Middle

Evenly throughout

Bottom

27 / 30

Category: Clinical Laboratory

What is the shelf life of properly stored Thioglycollate Broth?

Indefinite

2 years

1 month

months

28 / 30

Category: Clinical Laboratory

Thioglycollate Broth is NOT suitable for:

Sterility testing

Cultivating anaerobes

Differentiating oxygen requirements

Growing viruses

29 / 30

Category: Clinical Laboratory

What supplement might be needed for fastidious anaerobes?

Lactose

Hemin and vitamin K

Sodium chloride

Phenol red

30 / 30

Category: Clinical Laboratory

Microaerophiles grow best:

In a zigzag pattern

At the bottom

At the very top

Just below the surface

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Read Full Article: Thioglycollate Broth FAQs and MCQs

“How to read thioglycollate broth results”

“Thioglycollate vs cooked meat broth MCQs”

“Resazurin oxygen indicator interpretation”

“USP sterility testing protocol quiz”

Possible References Used

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Thioglycollate Broth MCQs Mock Test

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