Thioglycollate Broth MCQs Mock Test

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Test your microbiology expertise with our Thioglycollate Broth Mock Test! This 30-question quiz challenges your knowledge of this essential enriched medium used for culturing aerobic, anaerobic, and microaerophilic organisms.

🔬 What You’ll Be Tested On:

✔ Medium Composition & Principles

Sodium thioglycollate (reducing agent)
Resazurin (oxygen indicator)
Nutrient components (casein, yeast extract)

✔ Clinical Applications

Blood culture isolation
Wound/pus specimen processing
Sterility testing (USP <71>)

✔ Interpretation Skills

Oxygen gradient patterns (aerobic vs anaerobic growth)
Resazurin color changes
Contamination identification

✔ Troubleshooting

Pink discoloration causes
Overgrowth issues
Incubation optimization

🎯 Perfect For:

Medical lab scientists (MLS/MLT certification prep)
Microbiology students (USMLE, ASCP exams)
Clinical microbiologists

0 votes, 0 avg

Microbiology Department

Thioglycollate Broth MCQs Mock Test

🔬 What You’ll Be Tested On:

✔ Medium Composition & Principles

Sodium thioglycollate (reducing agent)
Resazurin (oxygen indicator)
Nutrient components (casein, yeast extract)

✔ Clinical Applications

Blood culture isolation
Wound/pus specimen processing
Sterility testing (USP <71>)

✔ Interpretation Skills

Oxygen gradient patterns (aerobic vs anaerobic growth)
Resazurin color changes
Contamination identification

✔ Troubleshooting

Pink discoloration causes
Overgrowth issues
Incubation optimization

🎯 Perfect For:

Medical lab scientists (MLS/MLT certification prep)
Microbiology students (USMLE, ASCP exams)
Clinical microbiologists

Read Full Article: Thioglycollate Broth FAQs and MCQs

1 / 30

Category: Clinical Laboratory

Facultative anaerobes show growth:

In a single colony

Only at the bottom

Only at the top

Throughout, but denser at the top

2 / 30

Category: Clinical Laboratory

What is the role of L-cystine in Thioglycollate Broth?

Neutralizes peroxides and supports anaerobes

Inhibits Gram-negative bacteria

Provides carbon source

Acts as a pH indicator

3 / 30

Category: Clinical Laboratory

Thioglycollate Broth is used for sterility testing of:

All of the above

Food samples

Blood cultures

Antibiotics

4 / 30

Category: Clinical Laboratory

Which bacteria would grow at the top of the tube?

Micrococcus luteus

Clostridium perfringens

Bacteroides vulgatus

Peptostreptococcus anaerobius

5 / 30

Category: Clinical Laboratory

What is the primary purpose of Thioglycollate Broth?

To inhibit fungal growth

To differentiate bacteria based on sugar fermentation

To selectively grow Gram-positive bacteria

To determine oxygen requirements of microorganisms

6 / 30

Category: Clinical Laboratory

What should be done if the medium turns pink before use?

Add more glucose

Discard it

Adjust pH with HCl

Reheat it to remove oxygen

7 / 30

Category: Clinical Laboratory

Which organism grows as “puffballs” in Thioglycollate Broth?

Pseudomonas aeruginosa

Bacteroides fragilis

E. coli

Streptococcus pneumoniae

8 / 30

Category: Clinical Laboratory

What is the purpose of the pancreatic digest of casein?

To act as a reducing agent

To inhibit fungi

To provide nitrogen and amino acids

To change color with pH

9 / 30

Category: Clinical Laboratory

What does flocculent growth in Thioglycollate Broth indicate?

No growth

Bacterial contamination

Fungal/yeast growth (e.g., Candida)

Viral infection

10 / 30

Category: Clinical Laboratory

Thioglycollate Broth is NOT suitable for:

Sterility testing

Differentiating oxygen requirements

Growing viruses

Cultivating anaerobes

11 / 30

Category: Clinical Laboratory

If the medium is pink throughout, it indicates:

Bacterial growth

Complete oxidation (unsuitable for anaerobes)

Proper anaerobic conditions

pH imbalance

12 / 30

Category: Clinical Laboratory

How should Thioglycollate Broth be stored?

In direct sunlight

Upright, sealed, and protected from light

With loose caps

Frozen

13 / 30

Category: Clinical Laboratory

Where do obligate aerobes grow in Thioglycollate Broth?

Bottom

Evenly throughout

Middle

Top

14 / 30

Category: Clinical Laboratory

Which regulatory body does NOT reference Thioglycollate Broth?

USP

European Pharmacopeia

APHA

CDC

15 / 30

Category: Clinical Laboratory

Which of the following is a limitation of Thioglycollate Broth?

Turns blue when sterile

Cannot support fastidious bacteria

Must be freshly prepared or reheated before use

Only grows aerobic bacteria

16 / 30

Category: Clinical Laboratory

Which standard recommends Thioglycollate Broth for sterility tests?

USP (United States Pharmacopeia)

CDC

WHO

FDA

17 / 30

Category: Clinical Laboratory

Obligate anaerobes grow predominantly in which zone?

Uniformly

Bottom

Surface

Middle

18 / 30

Category: Clinical Laboratory

Which organism is commonly tested in QC for Thioglycollate Broth?

Escherichia coli

Clostridium sporogenes

Staphylococcus epidermidis

Pseudomonas fluorescens

19 / 30

Category: Clinical Laboratory

Which ingredient in Thioglycollate Broth acts as a reducing agent?

Yeast extract

Agar

Glucose

Sodium thioglycollate

20 / 30

Category: Clinical Laboratory

Reheating Thioglycollate Broth multiple times can lead to:

Toxic oxygen radicals

Better anaerobiosis

Longer shelf life

Increased nutrient levels

21 / 30

Category: Clinical Laboratory

Why is sodium thioglycollate added to the medium?

To increase pH

To solidify the broth

To neutralize heavy metals

To inhibit Gram-positives

22 / 30

Category: Clinical Laboratory

How is Thioglycollate Broth sterilized?

UV radiation

Filtration

Autoclaving at 121°C for 15 minutes

Boiling for 5 minutes

23 / 30

Category: Clinical Laboratory

Why is agar (0.075%) sometimes added to Thioglycollate Broth?

To prevent convection currents

To solidify the medium

To inhibit anaerobes

To enhance bacterial motility

24 / 30

Category: Clinical Laboratory

What supplement might be needed for fastidious anaerobes?

Sodium chloride

Lactose

Hemin and vitamin K

Phenol red

25 / 30

Category: Clinical Laboratory

Why should Thioglycollate Broth not be stored at low temperatures?

Changes color

Increases oxygen absorption

Kills bacteria

It solidifies

26 / 30

Category: Clinical Laboratory

Microaerophiles grow best:

At the very top

At the bottom

In a zigzag pattern

Just below the surface

27 / 30

Category: Clinical Laboratory

What is the shelf life of properly stored Thioglycollate Broth?

months

2 years

1 month

Indefinite

28 / 30

Category: Clinical Laboratory

Why is blood diluted 1:10 in Thioglycollate Broth for cultures?

To inhibit bacteria

To reduce turbidity

To enhance oxygen levels

To prevent clotting

29 / 30

Category: Clinical Laboratory

What is the correct incubation temperature for Thioglycollate Broth?

25°C

42°C

30-35°C

4°C

30 / 30

Category: Clinical Laboratory

What does resazurin indicate in Thioglycollate Broth?

pH change

Protein digestion

Oxygen presence (pink = oxidized)

Glucose fermentation

Your score is

The average score is 52%

Read Full Article: Thioglycollate Broth FAQs and MCQs

“How to read thioglycollate broth results”

“Thioglycollate vs cooked meat broth MCQs”

“Resazurin oxygen indicator interpretation”

“USP sterility testing protocol quiz”

Possible References Used

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