Starch Casein Agar (SCA) MCQs Mock Test

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Challenge yourself with this 30-question mock test on Starch Casein Agar (SCA). Each MCQ is designed to test your understanding of SCA’s composition, uses, and application in microbiology labs.

🧪 Starch Casein Agar – 30 MCQs Mock Test

Instructions:
Choose the correct answer from the four options provided.

Go to Starch Casein Agar (SCA) FAQs and MCQs >>

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Microbiology Department

Starch Casein Agar (SCA) MCQs Mock Test

This practice test features 30 MCQs with answer keys to help lab professionals, students, and job seekers reinforce their understanding of Starch Casein Agar. A perfect tool for quick revision and interview preparation.

Read Full Article: Starch Casein Agar (SCA) FAQs and MCQs

1 / 30

Category: Clinical Laboratory

Which organism would NOT grow well on SCA?

Obligate anaerobes

Vibrio species

Streptomyces

Saccharolytic marine bacteria

2 / 30

Category: Clinical Laboratory

What color are actinomycete colonies on SCA?

Pink

Blue

White to light yellow

Green

3 / 30

Category: Clinical Laboratory

What should be done if SCA powder absorbs moisture?

Dry it in an oven

Both B & C

Discard it

Store in a tightly closed container

4 / 30

Category: Clinical Laboratory

What is the weight of SCA powder needed for 1L of media?

100 g

37 g

10 g

63 g

5 / 30

Category: Clinical Laboratory

Which of these is a key application of SCA?

Antibiotic discovery

Blood culture

Protozoan isolation

Viral plaque assay

6 / 30

Category: Clinical Laboratory

What confirms actinomycete identity after SCA isolation?

Gram staining & biochemical tests

Hemolysis on blood agar

Oxidase test

Motility test

7 / 30

Category: Clinical Laboratory

Why is KNO₃ added to SCA?

To increase viscosity

To inhibit gram-positive bacteria

For color indication

As a nitrogen source

8 / 30

Category: Clinical Laboratory

Why is autoclaving necessary for SCA?

To activate antibiotics

To sterilize and dissolve components

To melt the agar

To change the pH

9 / 30

Category: Clinical Laboratory

Which component in SCA provides sulfur?

KNO₃

MgSO₄·7H₂O

NaCl

CaCO₃

10 / 30

Category: Clinical Laboratory

Which salt in SCA acts as a pH buffer?

KNO₃

NaCl

CaCO₃

FeSO₄

11 / 30

Category: Clinical Laboratory

Which organism is MOST likely to grow on SCA?

Saccharomyces cerevisiae

Escherichia coli

Mycobacterium tuberculosis

Streptomyces praecox

12 / 30

Category: Clinical Laboratory

What is the role of casein in SCA?

Solidifying agent

Nitrogen source

pH indicator

Carbon source

13 / 30

Category: Clinical Laboratory

What is the colony morphology of Streptomyces praecox on SCA?

Green and fuzzy

Pink and irregular

Mucoid and translucent

Golden-yellow, circular, and opaque

14 / 30

Category: Clinical Laboratory

What is the sterilization method for SCA?

Filtration

Autoclaving at 121°C for 15 mins

Boiling for 5 mins

UV radiation

15 / 30

Category: Clinical Laboratory

Which component provides iron in SCA?

CaCO₃

FeSO₄·7H₂O

K₂HPO₄

MgSO₄·7H₂O

16 / 30

Category: Clinical Laboratory

Which of these is NOT a use of SCA?

Diagnosing tuberculosis

Studying soil microbes

Isolating marine bacteria

Preserving actinomycete cultures

17 / 30

Category: Clinical Laboratory

How does SCA help in environmental studies?

By detecting viral loads

By isolating pathogens from clinical samples

By identifying photosynthetic bacteria

By studying microbial decomposition of organic matter

18 / 30

Category: Clinical Laboratory

What is the solidifying agent in SCA?

Silica gel

Gelatin

Agar

Carrageenan

19 / 30

Category: Clinical Laboratory

What precaution prevents lump formation in SCA powder?

Avoiding moisture exposure

Keeping the container tightly closed

Storing in a dry place

All of the above

20 / 30

Category: Clinical Laboratory

Which component serves as the primary carbon source in SCA?

Starch

KNO₃

NaCl

Casein

21 / 30

Category: Clinical Laboratory

Which bacteria form mucoid colonies on SCA?

Vibrio cholerae

Bacillus subtilis

Streptococcus pneumoniae

Pseudomonas aeruginosa

22 / 30

Category: Clinical Laboratory

Why is seawater included in SCA?

To reduce pH

To inhibit fungal growth

To increase agar solidification

To provide essential nutrients for marine bacteria

23 / 30

Category: Clinical Laboratory

Which of the following is a limitation of SCA?

Turns blue upon bacterial growth

Supports only anaerobic bacteria

Poor growth for some strains due to nutritional variations

Cannot detect gram-negative bacteria

24 / 30

Category: Clinical Laboratory

What is the primary purpose of Starch Casein Agar (SCA)?

Isolation of actinomycetes

Cultivation of fungi

Growth of anaerobic bacteria

Detection of viruses

25 / 30

Category: Clinical Laboratory

What is the appearance of prepared SCA plates?

Clear to slightly opalescent

Blue and transparent

Red and opaque

Green and fluorescent

26 / 30

Category: Clinical Laboratory

Which of these is a HiMedia SCA ingredient?

Sea water (37 g/L)

Yeast extract

Glucose

Peptone

27 / 30

Category: Clinical Laboratory

What is the typical pH range of SCA?

5.0–6.0

7.0–7.3

6.0–6.5

8.0–9.0

28 / 30

Category: Clinical Laboratory

How should prepared SCA plates be stored?

At room temperature

Frozen at -20°C

At 2–8°C

Under UV light

29 / 30

Category: Clinical Laboratory

Which of these is NOT a component of SCA?

K₂HPO₄

MgSO₄

Glucose

Agar

30 / 30

Category: Clinical Laboratory

What is the incubation period for actinomycetes on SCA?

1–3 days

24 hours

1–7 days

10–14 days

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