Simple Staining MCQs Mock Test (30 MCQs)

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Prepare for your microbiology exams with our 30 Simple Staining MCQs Mock Test on LabTestsGuide.com! This quiz covers essential concepts, techniques, and applications of simple staining, helping you assess your knowledge and improve your understanding. Perfect for students, lab technicians, and medical professionals. Test yourself now and boost your confidence!

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Microbiology Department

Simple Staining MCQs Mock Test

Read Full Article: Simple Staining 50 FAQ and 40 MCQs

1 / 30

Category: Clinical Laboratory

Why do basic dyes adhere to bacterial cells?

Basic dyes are hydrophobic

Bacterial cells are negatively charged

Bacterial cells are positively charged

Basic dyes react with cell wall lipids

2 / 30

Category: Clinical Laboratory

Which staining technique uses only one dye?

Gram staining

Simple staining

Acid-fast staining

Endospore staining

3 / 30

Category: Clinical Laboratory

Which of the following is NOT a bacterial arrangement?

Diplo-

Staphylo-

Strepto-

Flagello-

4 / 30

Category: Clinical Laboratory

Which of the following is NOT a basic dye used in simple staining?

Carbol fuchsin

Nigrosin

Methylene blue

Crystal violet

5 / 30

Category: Clinical Laboratory

Why is heat fixation used in simple staining?

To sterilize the slide

To dissolve bacterial capsules

To enhance dye penetration

To kill bacteria and adhere them to the slide

6 / 30

Category: Clinical Laboratory

Which staining method differentiates Gram-positive and Gram-negative bacteria?

Gram staining

Acid-fast staining

Negative staining

Simple staining

7 / 30

Category: Clinical Laboratory

Why do cells wash off during staining?

Inadequate fixation

None of the above

Thin smear

Excessive rinsing

8 / 30

Category: Clinical Laboratory

What does “streptobacilli” arrangement refer to?

Chains of rods

Grape-like clusters

Clusters of rods

Pairs of spheres

9 / 30

Category: Clinical Laboratory

What happens if a slide is overheated during fixation?

Cells appear brighter

Cells may distort or rupture

Background becomes darker

Stain adheres better

10 / 30

Category: Clinical Laboratory

Which stain is NOT used in simple staining?

Iodine

Crystal violet

Methylene blue

Carbol fuchsin

11 / 30

Category: Clinical Laboratory

What type of staining colors the background instead of the cells?

Negative staining

Positive staining

Differential staining

Capsule staining

12 / 30

Category: Clinical Laboratory

What is the most common mistake in simple staining?

Using too little stain

Skipping fixation

Using acidic dyes

Applying too much bacterial culture

13 / 30

Category: Clinical Laboratory

How long should methylene blue be left on a smear?

b) 1–2 minutes

10–15 minutes

30–60 seconds

5–10 seconds

14 / 30

Category: Clinical Laboratory

Which staining technique is used for Mycobacterium?

Simple staining

Acid-fast staining

Gram staining

Negative staining

15 / 30

Category: Clinical Laboratory

What is the role of immersion oil in microscopy?

To sterilize the slide

To fix bacteria

To increase light refraction and resolution

To decolorize cells

16 / 30

Category: Clinical Laboratory

What is the primary stain in Gram staining?

Crystal violet

Methylene blue

Nigrosin

Safranin

17 / 30

Category: Clinical Laboratory

What is the charge of acidic dyes?

Negative

Variable

Neutral

Positive

18 / 30

Category: Clinical Laboratory

What shape are cocci bacteria?

Comma-shaped

Rod-shaped

Spherical

Spiral-shaped

19 / 30

Category: Clinical Laboratory

What is the primary purpose of simple staining?

To identify bacterial capsules

To visualize bacterial morphology and arrangement

To detect bacterial endospores

To differentiate Gram-positive and Gram-negative bacteria

20 / 30

Category: Clinical Laboratory

Which dye is commonly used for negative staining?

Safranin

Nigrosin

Crystal violet

b) Methylene blue

21 / 30

Category: Clinical Laboratory

Which dye is used for acid-fast staining?

Safranin

Carbol fuchsin

Crystal violet

Methylene blue

22 / 30

Category: Clinical Laboratory

Which bacteria retain crystal violet in Gram staining?

Gram-negative

Gram-positive

Capsulated

Acid-fast

23 / 30

Category: Clinical Laboratory

What causes uneven staining?

All of the above

Old dye

Thick smear

Incomplete washing

24 / 30

Category: Clinical Laboratory

Which arrangement describes “staphylococci”?

Tetrads of cocci

Clusters of cocci

Chains of cocci

Pairs of cocci

25 / 30

Category: Clinical Laboratory

What happens if decolorization is excessive in Gram staining?

Cells become colorless

No effect

Gram-negative cells appear Gram-positive

Gram-positive cells appear Gram-negative

26 / 30

Category: Clinical Laboratory

What shape are spirilla bacteria?

Filamentous

Spherical

Spiral

Rod-shaped

27 / 30

Category: Clinical Laboratory

What is the correct order of steps in simple staining

Smear preparation → Fixation → Staining → Washing

Washing → Fixation → Smear preparation → Staining

Staining → Washing → Fixation → Smear preparation

Fixation → Staining → Smear preparation → Washing

28 / 30

Category: Clinical Laboratory

Which microscope objective is used for final observation in simple staining?

10X

100X (oil immersion)

40X

29 / 30

Category: Clinical Laboratory

What is the counterstain in Gram staining?

Methylene blue

Safranin

Crystal violet

Carbol fuchsin

30 / 30

Category: Clinical Laboratory

Why might bacteria not appear stained after simple staining?

Insufficient staining time

Overheating

Using too much water

All of the above

Your score is

The average score is 57%

Read Full Article: Simple Staining 50 FAQ and 40 MCQs

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