Simple Staining MCQs Mock Test (30 MCQs)

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Prepare for your microbiology exams with our 30 Simple Staining MCQs Mock Test on LabTestsGuide.com! This quiz covers essential concepts, techniques, and applications of simple staining, helping you assess your knowledge and improve your understanding. Perfect for students, lab technicians, and medical professionals. Test yourself now and boost your confidence!

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Microbiology Department

Simple Staining MCQs Mock Test

Read Full Article: Simple Staining 50 FAQ and 40 MCQs

1 / 30

Category: Clinical Laboratory

How long should methylene blue be left on a smear?

b) 1–2 minutes

5–10 seconds

30–60 seconds

10–15 minutes

2 / 30

Category: Clinical Laboratory

What is the correct order of steps in simple staining

Staining → Washing → Fixation → Smear preparation

Washing → Fixation → Smear preparation → Staining

Fixation → Staining → Smear preparation → Washing

Smear preparation → Fixation → Staining → Washing

3 / 30

Category: Clinical Laboratory

What does “streptobacilli” arrangement refer to?

Chains of rods

Pairs of spheres

Clusters of rods

Grape-like clusters

4 / 30

Category: Clinical Laboratory

What shape are cocci bacteria?

Spherical

Rod-shaped

Spiral-shaped

Comma-shaped

5 / 30

Category: Clinical Laboratory

Which stain is NOT used in simple staining?

Iodine

Crystal violet

Carbol fuchsin

Methylene blue

6 / 30

Category: Clinical Laboratory

What is the primary purpose of simple staining?

To detect bacterial endospores

To visualize bacterial morphology and arrangement

To identify bacterial capsules

To differentiate Gram-positive and Gram-negative bacteria

7 / 30

Category: Clinical Laboratory

Why is heat fixation used in simple staining?

To sterilize the slide

To enhance dye penetration

To kill bacteria and adhere them to the slide

To dissolve bacterial capsules

8 / 30

Category: Clinical Laboratory

What happens if a slide is overheated during fixation?

Cells appear brighter

Stain adheres better

Background becomes darker

Cells may distort or rupture

9 / 30

Category: Clinical Laboratory

Which dye is used for acid-fast staining?

Methylene blue

Carbol fuchsin

Crystal violet

Safranin

10 / 30

Category: Clinical Laboratory

What is the counterstain in Gram staining?

Carbol fuchsin

Crystal violet

Methylene blue

Safranin

11 / 30

Category: Clinical Laboratory

Why do basic dyes adhere to bacterial cells?

Bacterial cells are negatively charged

Basic dyes react with cell wall lipids

Basic dyes are hydrophobic

Bacterial cells are positively charged

12 / 30

Category: Clinical Laboratory

Which staining technique is used for Mycobacterium?

Negative staining

Acid-fast staining

Simple staining

Gram staining

13 / 30

Category: Clinical Laboratory

What is the most common mistake in simple staining?

Skipping fixation

Using too little stain

Applying too much bacterial culture

Using acidic dyes

14 / 30

Category: Clinical Laboratory

Which staining technique uses only one dye?

Endospore staining

Gram staining

Simple staining

Acid-fast staining

15 / 30

Category: Clinical Laboratory

What type of staining colors the background instead of the cells?

Positive staining

Capsule staining

Differential staining

Negative staining

16 / 30

Category: Clinical Laboratory

What happens if decolorization is excessive in Gram staining?

Cells become colorless

Gram-positive cells appear Gram-negative

No effect

Gram-negative cells appear Gram-positive

17 / 30

Category: Clinical Laboratory

What shape are spirilla bacteria?

Rod-shaped

Filamentous

Spherical

Spiral

18 / 30

Category: Clinical Laboratory

Which bacteria retain crystal violet in Gram staining?

Capsulated

Acid-fast

Gram-negative

Gram-positive

19 / 30

Category: Clinical Laboratory

What is the role of immersion oil in microscopy?

To decolorize cells

To fix bacteria

To increase light refraction and resolution

To sterilize the slide

20 / 30

Category: Clinical Laboratory

Why do cells wash off during staining?

Excessive rinsing

Thin smear

None of the above

Inadequate fixation

21 / 30

Category: Clinical Laboratory

What is the charge of acidic dyes?

Variable

Negative

Positive

Neutral

22 / 30

Category: Clinical Laboratory

Which of the following is NOT a bacterial arrangement?

Diplo-

Staphylo-

Flagello-

Strepto-

23 / 30

Category: Clinical Laboratory

Which dye is commonly used for negative staining?

Nigrosin

Safranin

Crystal violet

b) Methylene blue

24 / 30

Category: Clinical Laboratory

Which staining method differentiates Gram-positive and Gram-negative bacteria?

Acid-fast staining

Negative staining

Simple staining

Gram staining

25 / 30

Category: Clinical Laboratory

Which arrangement describes “staphylococci”?

Tetrads of cocci

Clusters of cocci

Pairs of cocci

Chains of cocci

26 / 30

Category: Clinical Laboratory

What is the primary stain in Gram staining?

Nigrosin

Safranin

Methylene blue

Crystal violet

27 / 30

Category: Clinical Laboratory

Why might bacteria not appear stained after simple staining?

All of the above

Insufficient staining time

Overheating

Using too much water

28 / 30

Category: Clinical Laboratory

What causes uneven staining?

Incomplete washing

All of the above

Thick smear

Old dye

29 / 30

Category: Clinical Laboratory

Which microscope objective is used for final observation in simple staining?

10X

40X

100X (oil immersion)

30 / 30

Category: Clinical Laboratory

Which of the following is NOT a basic dye used in simple staining?

Carbol fuchsin

Nigrosin

Crystal violet

Methylene blue

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Read Full Article: Simple Staining 50 FAQ and 40 MCQs

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