Sabouraud Dextrose Agar (SDA) MCQs Mock Test

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Test your mycology expertise with our SDA Mock Test! This 30-question quiz evaluates your ability to:

✔ Interpret Fungal Growth Patterns:

Yeasts (Candida creamy colonies)
Molds (Aspergillus velvety texture)
Dermatophytes (Microsporum powdery appearance)

✔ Master SDA Fundamentals:

Low pH (5.6) for bacterial inhibition
Dextrose-rich formulation
Antibiotic-supplemented variants

✔ Troubleshoot Common Issues:

Bacterial contamination
Media dehydration
Slow-growing fungi

🔬 Key Features:
✓ Image-Based Questions – Analyze real SDA cultures
✓ Detailed Explanations – Learn while testing
✓ Instant Scoring – Benchmark your performance
✓ Mobile-Optimized – Study on-the-go

🎯 Perfect For:

Medical Lab Technicians (MLT/MLT certification)
Microbiology Students (USMLE, ASCP prep)
Dermatology & Mycology Specialists

0 votes, 0 avg

Microbiology Department

Sabouraud Dextrose Agar (SDA) MCQs Mock Test

Test your mycology expertise with our SDA Mock Test! This 30-question quiz evaluates your ability to:

✔ Interpret Fungal Growth Patterns:

Yeasts (Candida creamy colonies)
Molds (Aspergillus velvety texture)
Dermatophytes (Microsporum powdery appearance)

✔ Master SDA Fundamentals:

Low pH (5.6) for bacterial inhibition
Dextrose-rich formulation
Antibiotic-supplemented variants

✔ Troubleshoot Common Issues:

Bacterial contamination
Media dehydration
Slow-growing fungi

🎯 Perfect For:

Medical Lab Technicians (MLT/MLT certification)
Microbiology Students (USMLE, ASCP prep)
Dermatology & Mycology Specialists

Read Full Article: Sabouraud Dextrose Agar (SDA) FAQs and MCQs

1 / 30

Category: Clinical Laboratory

What is the purpose of cycloheximide in SDA?

Inhibit saprophytic fungi

Solidify the medium

Enhance pigment production

Promote bacterial growth

2 / 30

Category: Clinical Laboratory

What is the shelf life of prepared SDA plates?

6 months

1 week

1 day

A few days (if stored at 4°C)

3 / 30

Category: Clinical Laboratory

What is the solidifying agent in SDA?

Pectin

Silica gel

Gelatin

Agar

4 / 30

Category: Clinical Laboratory

What is the reverse color of Aspergillus fumigatus on SDA?

Black

Blue

Pale yellow

Red

5 / 30

Category: Clinical Laboratory

Why should SDA not be re-melted?

It becomes toxic

Changes color

Loses antibiotics

Agar degrades and softens

6 / 30

Category: Clinical Laboratory

Which fungus appears as “salt and pepper” on SDA?

Penicillium spp.

Candida albicans

Aspergillus niger

Aspergillus flavus

7 / 30

Category: Clinical Laboratory

Which fungus is unsafe for teaching labs?

Rhizopus stolonifer

Saccharomyces cerevisiae

Aspergillus fumigatus

Penicillium camemberti

8 / 30

Category: Clinical Laboratory

Which antibiotic is commonly added to SDA to inhibit bacteria?

Streptomycin

Penicillin

Chloramphenicol

Vancomycin

9 / 30

Category: Clinical Laboratory

Why is SDA used in food testing?

To enumerate yeasts and molds

To detect bacterial toxins

To identify viruses

To culture probiotics

10 / 30

Category: Clinical Laboratory

How long should SDA plates be incubated for yeast detection?

1 week

1 month

2–3 days

24 hours

11 / 30

Category: Clinical Laboratory

Who developed Sabouraud Dextrose Agar (SDA)?

Louis Pasteur

Alexander Fleming

Raymond Sabouraud

Robert Koch

12 / 30

Category: Clinical Laboratory

Which bacteria are inhibited by gentamicin in SDA?

All bacteria

Acid-fast bacilli

Gram-positive bacteria

Gram-negative bacteria

13 / 30

Category: Clinical Laboratory

What is the heating time for 200 mL SDA in a water bath?

5 minutes

45 minutes

2 hours

10 seconds

14 / 30

Category: Clinical Laboratory

Which organism is a QC strain for SDA?

Staphylococcus aureus

Aspergillus brasiliensis (ATCC 16404)

Pseudomonas aeruginosa

Escherichia coli

15 / 30

Category: Clinical Laboratory

What happens if SDA is overheated during preparation?

Loses dextrose

Becomes too soft

Kills all fungi

Turns black

16 / 30

Category: Clinical Laboratory

Which modification of SDA has a neutral pH?

MacConkey-SDA blend

Blood agar variant

Original Sabouraud formula

Emmons modification

17 / 30

Category: Clinical Laboratory

What does Geotrichum candidum look like on SDA?

Green powdery growth

Red pigmentation

Black spores

White, flat colonies with aerial mycelium

18 / 30

Category: Clinical Laboratory

What is the primary purpose of SDA?

Protozoan growth

Isolation of fungi and yeasts

Viral culture

Cultivation of bacteria

19 / 30

Category: Clinical Laboratory

How should SDA plates be disposed of?

Burn openly

Autoclave at 121°C for 20 minutes

Throw in regular trash

Freeze at -20°C

20 / 30

Category: Clinical Laboratory

Which organism requires thiamine for growth on SDA?

Penicillium spp.

Trichophyton verrucosum

Aspergillus niger

Candida albicans

21 / 30

Category: Clinical Laboratory

What is the incubation temperature for molds on SDA?

37°C

4°C

22–25°C (Room temperature)

55°C

22 / 30

Category: Clinical Laboratory

What is the pH of standard SDA?

8.2

7.0

6.5

5.6

23 / 30

Category: Clinical Laboratory

What is the main nitrogen source in SDA?

Agar

Peptone

Chloramphenicol

Dextrose

24 / 30

Category: Clinical Laboratory

Which incubation temperature is used for dimorphic fungi?

30°C and 37°C

50°C

22°C

4°C

25 / 30

Category: Clinical Laboratory

What is the colony morphology of Candida albicans on SDA?

Green powdery colonies

Yellow pigmentation

Creamy, white, smooth colonies

Black spores

26 / 30

Category: Clinical Laboratory

Which fungus grows as white, wrinkled colonies on SDA?

Penicillium spp.

Candida albicans

Aspergillus niger

Trichosporon mucoides

27 / 30

Category: Clinical Laboratory

Which fungus produces yellow-green colonies on SDA?

Geotrichum candidum

Trichosporon spp.

Aspergillus flavus

Candida albicans

28 / 30

Category: Clinical Laboratory

Which component in SDA acts as a carbon source?

Agar

Chloramphenicol

Dextrose (Glucose)

Peptone

29 / 30

Category: Clinical Laboratory

What inhibits bacterial growth in SDA?

Lack of oxygen

Low pH and antibiotics

UV light exposure

High sugar and neutral pH

30 / 30

Category: Clinical Laboratory

Which standard recommends SDA for microbial testing?

US Pharmacopeia (USP)

CLSI

FDA

WHO

Your score is

The average score is 45%

“How to identify Candida on SDA”
“SDA incubation temperature for molds”
“SDA vs. CHROMagar MCQs”
“Fungal culture troubleshooting”

Read Full Article: Sabouraud Dextrose Agar (SDA) FAQs and MCQs

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