Microbial Culture Media MCQs 30 Mock Test

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Master Microbial Culture Media with Mock Test of 30 MCQs! Covers types (selective, differential, enriched), preparation (pH, sterilization), and uses (Blood Agar, MacConkey, Chocolate Agar). Learn about solid vs. liquid media, fastidious bacteria, and antibiotic testing. Essential for microbiology students, lab techs, and researchers!

Read the Full Article 50 FAQs and 30 MCQs >>

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Microbial Culture Media MCQs Mock Test

Master Microbial Culture Media with 50 FAQs & 30 MCQs! Covers types (selective, differential, enriched), preparation (pH, sterilization), and uses (Blood Agar, MacConkey, Chocolate Agar). Learn about solid vs. liquid media, fastidious bacteria, and antibiotic testing. Essential for microbiology students, lab techs, and researchers!

Key Highlights:

✔ Types of Media – Selective (MacConkey), Differential (Blood Agar), Enriched (Chocolate Agar)
✔ Preparation Steps – Autoclaving (121°C, 15 psi), pH Adjustment, Agar Concentration (1.5-2%)
✔ Special Media – Lowenstein-Jensen (TB), Sabouraud (Fungi), TCBS (Vibrio), Cetrimide (Pseudomonas)
✔ MCQs for Exams – Antibiotic Sensitivity (Mueller-Hinton), Motility (Semi-Solid), Fastidious Bacteria (X & V Factors)
✔ Common Issues – Contamination, Media Not Solidifying, No Bacterial Growth

Perfect for: Medical labs, microbiology courses, and clinical research. 🧫🔬

Read Full Article: Microbial Culture Media FAQs and MCQs

1 / 29

Category: Clinical Laboratory

What is the role of bile salts in MacConkey agar?

To enhance fungal growth

To inhibit Gram-positive bacteria

To increase pH

To solidify media

2 / 29

Category: Clinical Laboratory

What is the indicator in Mannitol Salt Agar (MSA)?

Bromothymol blue

Phenol red

Methylene blue

Neutral red

3 / 29

Category: Clinical Laboratory

Which media is used to differentiate lactose fermenters from non-fermenters?

Nutrient agar

Blood agar

Sabouraud agar

MacConkey agar

4 / 29

Category: Clinical Laboratory

What is the concentration of agar in solid media?

1.5-2.0%

5-10%

20-30%

0.1-0.5%

5 / 29

Category: Clinical Laboratory

What is the purpose of Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) agar?

To isolate Vibrio species

To detect E. coli

To test antibiotic resistance

To grow fungi

6 / 29

Category: Clinical Laboratory

Why are Petri plates inverted after pouring media?

To speed up solidification

To enhance bacterial growth

To reduce contamination

To prevent condensation from dripping

7 / 29

Category: Clinical Laboratory

Mannitol Salt Agar (MSA) is selective for:

E. coli

c) Pseudomonas aeruginosa

Staphylococcus aureus

Salmonella typhi

8 / 29

Category: Clinical Laboratory

Which media is used to isolate fastidious bacteria like Neisseria gonorrhoeae?

Mannitol salt agar

Chocolate agar

Nutrient agar

MacConkey agar

9 / 29

Category: Clinical Laboratory

MacConkey agar is selective for:

Anaerobes

Fungi

Gram-positive bacteria

Gram-negative bacteria

10 / 29

Category: Clinical Laboratory

What is the purpose of enrichment media like Selenite F broth?

To selectively enhance growth of pathogens (e.g., Salmonella)

To solidify media

To stain bacteria

To kill all bacteria

11 / 29

Category: Clinical Laboratory

What does a zone of inhibition indicate in antibiotic testing?

Sensitivity to antibiotics

Resistance to antibiotics

Contamination

Bacterial growth

12 / 29

Category: Clinical Laboratory

Why might prepared media appear cloudy?

All of the above

Due to undissolved agar

Due to bacterial contamination

Because of improper sterilization

13 / 29

Category: Clinical Laboratory

Which instrument is used to sterilize media?

Centrifuge

Incubator

Spectrophotometer

Autoclave

14 / 29

Category: Clinical Laboratory

What is the primary purpose of microbial culture media?

To observe viruses

To stain bacteria

To kill microorganisms

To support microbial growth

15 / 29

Category: Clinical Laboratory

What is the function of peptone in culture media?

Source of carbon and nitrogen

Selective agent

Solidifying agent

pH indicator

16 / 29

Category: Clinical Laboratory

Which medium is used for the cultivation of Legionella?

Nutrient agar

BCYE agar

Blood agar

MacConkey agar

17 / 29

Category: Clinical Laboratory

What is the standard autoclaving condition for sterilizing media?

60°C for 1 hour

121°C for 15 min at 15 psi

80°C for 30 min

100°C for 5 min

18 / 29

Category: Clinical Laboratory

Which medium is used to test for Clostridium difficile?

Blood agar

Nutrient agar

Cycloserine-cefoxitin-fructose agar (CCFA)

MacConkey agar

19 / 29

Category: Clinical Laboratory

Which medium is used to transport Vibrio cholerae samples?

Cary-Blair medium

Nutrient agar

Blood agar

Chocolate agar

20 / 29

Category: Clinical Laboratory

Which of the following is NOT a step in media preparation?

Pouring into Petri plates

Weighing ingredients

Autoclaving

Adding antibiotics before sterilization

21 / 29

Category: Clinical Laboratory

Which medium is used to grow Mycobacterium tuberculosis?

Blood agar

Sabouraud agar

MacConkey agar

Lowenstein-Jensen medium

22 / 29

Category: Clinical Laboratory

Which medium is used for antibiotic sensitivity testing?

MacConkey agar

Chocolate agar

Mueller-Hinton agar

Blood agar

23 / 29

Category: Clinical Laboratory

Which component acts as a solidifying agent in solid media?

Peptone

Agar

Yeast extract

Beef extract

24 / 29

Category: Clinical Laboratory

Which medium is used to test water for coliforms?

MacConkey broth

Blood agar

Chocolate agar

Nutrient agar

25 / 29

Category: Clinical Laboratory

What is the purpose of adjusting pH in media preparation?

To optimize microbial growth

To make it colorful

To increase agar concentration

To prevent evaporation

26 / 29

Category: Clinical Laboratory

Which medium is used for coagulase test in Staphylococcus?

Nutrient agar

Blood agar

MacConkey agar

Rabbit plasma agar

27 / 29

Category: Clinical Laboratory

What is the purpose of resuscitation media?

To solidify media faster

To recover stressed/damaged bacteria

To kill bacteria

To stain bacteria

28 / 29

Category: Clinical Laboratory

Which of the following is a liquid medium?

Nutrient agar

Blood agar

Nutrient broth

MacConkey agar

29 / 29

Category: Clinical Laboratory

Which medium is used to detect Pseudomonas aeruginosa?

Blood agar

MacConkey agar

Cetrimide agar

Sabouraud agar

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Read the Full Article 50 FAQs and 30 MCQs >>

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