Masson’s Trichrome staining MCQs Mock Test (30 MCQs)

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Master Masson’s Trichrome staining, the gold-standard technique for connective tissue differentiation in histopathology. This comprehensive guide covers:
✓ Tri-color principle (collagen, muscle, nuclei staining)
✓ Step-by-step protocol with fixation requirements
✓ Key diagnostic applications:

Liver fibrosis staging
Muscle biopsy analysis
Tumor stroma evaluation
✓ Troubleshooting uneven staining

Essential for pathologists, histotechnologists, and researchers in fibrosis studies and musculoskeletal diagnostics.

Test your histology expertise with 35+ Masson’s Trichrome MCQs covering:
◼️ Color interpretation (blue vs. red vs. black)
◼️ Fibrosis grading in liver/kidney biopsies
◼️ Artifacts and fixation requirements
◼️ Comparison with Van Gieson & Sirius Red stains

Ideal for pathology boards, histotechnology certification, and research labs.

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Cytology and Histology Department

Masson’s Trichrome Staining MCQs Mock Test

Ideal for pathology boards, histotechnology certification, and research labs.

Read Full Article: Masson’s Trichrome staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

How long should sections be mordanted in Bouin’s solution using the microwave method?

5 minutes

1 minute

15 minutes

10 minutes

2 / 30

Category: Clinical Laboratory

What color does collagen stain in Masson’s Trichrome Stain?

Green

Blue

Red

Black

3 / 30

Category: Clinical Laboratory

What PPE is required for handling Masson’s Trichrome Stain reagents?

Gloves, goggles, and lab coat

Gloves and goggles

No PPE is required

Lab coat and mask

4 / 30

Category: Clinical Laboratory

What is the shelf life of 1% acetic acid?

1 year

3-4 days

1 month

6 months

5 / 30

Category: Clinical Laboratory

Which type of biopsies is Masson’s Trichrome Stain routinely used for?

Skin and bone biopsies

Brain and spinal cord biopsies

Liver and kidney biopsies

Lung and heart biopsies

6 / 30

Category: Clinical Laboratory

Which reagent is both a skin and eye irritant?

Biebrich Scarlet

Phosphotungstic acid

Aniline Blue

All of the above

7 / 30

Category: Clinical Laboratory

What is the thickness of paraffin sections recommended for Masson’s Trichrome Stain?

4 micrometers

10 micrometers

6 micrometers

2 micrometers

8 / 30

Category: Clinical Laboratory

What is the purpose of rinsing with 1% acetic acid?

To fix the tissue

To stain nuclei

To differentiate the stain and remove excess dye

To dehydrate the tissue

9 / 30

Category: Clinical Laboratory

What is the preferred collagen color for liver biopsies?

Black

Red

Dark blue

Light blue

10 / 30

Category: Clinical Laboratory

What is the hazard associated with picric acid?

It is a skin irritant

It can become explosive when dry

It is non-toxic

It is flammable

11 / 30

Category: Clinical Laboratory

What color do nuclei stain in Masson’s Trichrome Stain?

Black

Green

Blue

Red

12 / 30

Category: Clinical Laboratory

How long is Bouin’s fixative stable?

2 years

1 year

6 months

Indefinitely

13 / 30

Category: Clinical Laboratory

What is the shelf life of Weigert’s Iron Hematoxylin working solution?

3-4 days

1 year

1 month

6 months

14 / 30

Category: Clinical Laboratory

What is the purpose of dehydrating and clearing the sections?

To fix the tissue

To prepare the tissue for mounting

To stain the tissue

To differentiate the stain

15 / 30

Category: Clinical Laboratory

Which dye is used to stain collagen blue in Masson’s Trichrome Stain?

Aniline Blue

Biebrich Scarlet

Light Green

Weigert's Iron Hematoxylin

16 / 30

Category: Clinical Laboratory

Which component of Bouin’s fixative is a known carcinogen?

Picric acid

Acetic acid

Hematoxylin

Formaldehyde

17 / 30

Category: Clinical Laboratory

What is the role of phosphotungstic/phosphomolybdic acid in the staining process?

To stain nuclei

To fix the tissue

To differentiate collagen by pulling out red dye

To dehydrate the tissue

18 / 30

Category: Clinical Laboratory

What is the primary purpose of Masson’s Trichrome Stain?

To stain lipids

To differentiate between collagen and smooth muscle

To stain nuclei

To identify bacterial infections

19 / 30

Category: Clinical Laboratory

What is the composition of Bouin’s fixative?

Hematoxylin, eosin, and alcohol

Phosphotungstic acid, acetic acid, and water

Aniline Blue, Biebrich Scarlet, and water

Picric acid, formaldehyde, and acetic acid

20 / 30

Category: Clinical Laboratory

What color do cytoplasm and muscle stain in Masson’s Trichrome Stain?

Black

Red

Green

Blue

21 / 30

Category: Clinical Laboratory

What is the principle behind Masson’s Trichrome Stain?

It uses three dyes to stain tissues based on porosity and molecular size

It uses heat to differentiate tissues

It uses antibodies to bind to specific tissues

It uses enzymes to break down tissues

22 / 30

Category: Clinical Laboratory

What is the shelf life of Aniline Blue solution?

1 month

1 year

6 months

3-4 days

23 / 30

Category: Clinical Laboratory

What is the conventional method for mordanting in Bouin’s solution?

Heat at 37°C for 30 minutes

Heat at 60°C for 1 hour

Microwave for 1 minute

Leave at room temperature for 24 hours

24 / 30

Category: Clinical Laboratory

Which dye can be substituted for Aniline Blue?

Light Green

Hematoxylin

Biebrich Scarlet

Eosin

25 / 30

Category: Clinical Laboratory

Which of the following is a component of Biebrich Scarlet solution?

Light Green

Phosphotungstic acid

Acid Fuchsin

Hematoxylin

26 / 30

Category: Clinical Laboratory

Which reagent is used to stain erythrocytes red?

Weigert's Iron Hematoxylin

Aniline Blue

Phosphotungstic acid

Biebrich Scarlet

27 / 30

Category: Clinical Laboratory

How long should sections be stained with Weigert’s working hematoxylin?

10 minutes

15 minutes

20 minutes

5 minutes

28 / 30

Category: Clinical Laboratory

What is the preferred fixative for Masson’s Trichrome Stain?

Ethanol

10% formalin

Methanol

Bouin's fixative

29 / 30

Category: Clinical Laboratory

Why should Bouin’s fixative be kept under a fume hood?

To avoid contamination

To avoid inhalation of formaldehyde fumes

To keep it cold

To prevent evaporation

30 / 30

Category: Clinical Laboratory

Which reagent is used to differentiate collagen in Masson’s Trichrome Stain?

Biebrich Scarlet

Acetic acid

Weigert's Iron Hematoxylin

Phosphotungstic/Phosphomolybdic acid

Your score is

The average score is 35%

Read Full Article: Masson’s Trichrome staining 50 FAQ and 30 MCQs

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