Latent Tuberculosis Infection (LTBI) Test used in the diagnosis of tuberculosis (TB). It is an extract of proteins derived from the bacterium Mycobacterium tuberculosis, which causes TB. Purified Protein Derivative (PPD) is injected under the skin, typically on the forearm, and the reaction to the injection is observed after a specific period of time.

The PPD test, also known as the tuberculin skin test or Mantoux test, is used to determine if a person has been exposed to the TB bacteria. The test works by measuring the immune response to the proteins in the PPD solution. If a person has been infected with TB in the past, their immune system will have developed a delayed-type hypersensitivity reaction to the proteins in the PPD, leading to a localized skin reaction at the injection site.

Principle:

• PPD (purified protein derivative) is subcutaneously injected to the patient and individual’s sensitivity to tuberculin protein is assessed. If the patient already had a previous exposure to M. tuberculosis, a hypersensitivity reaction takes place.
• The antigen produces induration (thickening) at the site of injection. The diameter of the induration is measured to evaluate the test and is directly proportional to the level of sensitization. American Thoracic Society (ATS) has recommended 5 Tuberculin Units (0.1 ml) as a standard regimen for the Mantoux Test.
• In highly endemic areas, this standard dose could be reduced as per the guidelines.

Requirements:

• Graduated syringe (of 1 ml) with a short bevel needle (26 G)
• Tuberculin PPD (Mantoux solution)
• Spirit swab

Procedure:

1. Bring the tuberculin PPD to room temperature.
2. Draw up just over 0.1ml of tuberculin protein into a graduated syringe.
3. Remove air and excess tuberculin to leave exactly 0.1ml of tuberculin.
4. The ideal site is dorsal surface of the forearm (usually left) about 4 cm below the elbow joint.
5. Clean the site with spirit swab and allow to dry.
6. Insert the needle parallel with the skin surface, advance the bevel 3-5 mm and inject the PPD.
7. Remove the needle without pressing the injection site.
8. Wait for 48-72 hours and observe the induration. The area of erythema is not included.

Reading the TB skin test:

“Reading” the skin test means detecting a raised, thickened local area of skin reaction, referred to as induration. Induration is the key item to detect, not redness or bruising. Read skin tests 48-72 hours after the injection when the size of the induration is maximal. Tests read after 72 hours tend to underestimate the size of the induration and are not accurate

• The negative case when there is no induration.
• In positive cases, there will be induration at the site of injection appear in 48 to 72 hours.
• Induration < 5 mm D is seen in:
  • HIV patients or immunodeficiency state.
  • Close contact with active TB case.
  • The patient with X-Ray finding of old TB.
• Induration > 10 mm in D seen in:
  • IV drug users.
  • Nursing home residents.
  • Traveler from high TB areas.
  • A worker in the home care facility.
  • Malnutrition, postgastrectomy, steroid use and Diabetes.
• Induration > 15 mm in diameter seen in:
  • Patient with active TB.
  • All patients who don’t fulfill the above criteria.

The positive test is seen in:

1. An active case of TB.
2. Other types of Mycobacterial infection.

The negative test is seen in:

1. Immune incompetent chronically ill patient.
2. Patient not exposed to TB.

INTERPRETATION OF MANTOUX TEST:

1. A positive Mantoux test indicates a previous exposure to M. tuberculosis but not necessarily an active disease.
2. The Mantoux test causes a booster effect to produce a positive result in an individual who has been already exposed previously.
3. The Mantoux test usually becomes positive 4-6 weeks after infection.
4. If a papule does not appear after the injection, the test should be repeated on other arm as the solution has been injected too deeply.
5. False negative results are seen if there is bacterial contamination of the tuberculin solution, corticosteroid therapy, viral infections (HIV, influenza, EBV) and poor nutrition.
6. False positive results are seen in individuals vaccinated with BCG (reactions are 5-10 mm diameter).
7. If the induration is 15 mm or more in BCG vaccinated individual, it should be considered positive.

Possible References Used

⚠️ Disclaimer:

The content on LabTestsGuide.com is for informational and educational purposes only. We do not guarantee the accuracy, completeness, or timeliness of the information provided. Always consult qualified healthcare professionals for medical advice, diagnosis, or treatment. LabTestsGuide.com is not liable for any decisions made based on the information on this site.