Giemsa Stain MCQs Mock Test (30 MCQs)

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Master Giemsa staining, the essential technique for blood smears and parasite identification. This guide covers:
✓ Romanowsky stain principle (azure-eosin combo)
✓ Step-by-step protocol for peripheral blood smears
✓ Key applications in:

Malaria diagnosis (Plasmodium species)
Hematology (WBC differentials, platelet estimation)
Bacteriology (Helicobacter pylori, Borrelia)
✓ Troubleshooting uneven staining

Ideal for hematologists, microbiologists, and lab technicians preparing for clinical exams.

Challenge yourself with 30+ Giemsa staining MCQs covering:
◼️ Identifying malarial parasites (ring forms vs. gametocytes)
◼️ Interpreting atypical lymphocytes in viral infections
◼️ Distinguishing granulocytes vs. agranulocytes
◼️ Common artifacts and fixes

Perfect for USMLE, ASCP, and tropical medicine certification with instant scoring.

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Hematology Department

Giemsa Stain MCQs Mock Test

Challenge yourself with 30 Giemsa staining MCQs covering:
◼️ Identifying malarial parasites (ring forms vs. gametocytes)
◼️ Interpreting atypical lymphocytes in viral infections
◼️ Distinguishing granulocytes vs. agranulocytes
◼️ Common artifacts and fixes

Perfect for USMLE, ASCP, and tropical medicine certification with instant scoring.

Read Full Article: Giemsa Stain FAQs and MCQs 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

Why is buffered water used in Giemsa staining?

To decrease the pH

To maintain the pH at 7.2

To act as a fixative

To increase the pH

2 / 30

Category: Clinical Laboratory

What is the primary hazard of methanol in Giemsa staining?

It is explosive

It is corrosive

It is flammable and toxic

It is radioactive

3 / 30

Category: Clinical Laboratory

What is the primary use of Giemsa stain?

Staining plant cells

Diagnosing malaria and blood parasites

Staining fungal hyphae

Identifying viruses

4 / 30

Category: Clinical Laboratory

What causes iridescent scum on Giemsa stain?

Contamination with bacteria

Improper filtration

High pH

Absorption of water vapor

5 / 30

Category: Clinical Laboratory

What is the pH required for Giemsa staining?

5.0

8.0

9.5

6.8 or 7.2

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Category: Clinical Laboratory

What is the shelf life of Giemsa stock solution?

1 month

1 week

Indefinite if stored properly

6 months

7 / 30

Category: Clinical Laboratory

What type of stain is Giemsa?

Basic stain

Fluorescent stain

Acidic stain

Neutral stain (Romanowsky stain)

8 / 30

Category: Clinical Laboratory

What color do erythrocytes appear after Giemsa staining?

Green

Blue

Purple

Pink

9 / 30

Category: Clinical Laboratory

What is the role of a positive control smear?

Purple granules

Red

How long is a thin blood film stained with Giemsa? a) 5-10 minutes b) 20-30 minutes✔ c) 45-60 minutes d) 2 hours What is the purpose of Triton X-100 in Giemsa staining? a) To fix the smear b) To evenly distribute the stain✔ c) To act as a buffer d) To decolorize the slide How are thick smears rinsed after staining? a) Dipped 3-4 times in buffer b) Left in buffer for 5 minutes✔ c) Washed with tap water d) Rinsed with methanol What is the purpose of leaving duplicate smears unstained? a) To save staining reagents b) To serve as backups or for reference labs✔ c) To reduce staining time d) To avoid contamination What is the role of a positive control smear? a) To test the pH of the stain b) To ensure proper staining results✔ c) To fix the smear d) To dilute the stain What color do erythrocytes appear after Giemsa staining? a) Blue b) Pink✔ c) Purple d) Green What color is the nucleus of blood cells after Giemsa staining? a) Red b) Blue-purple✔ c) Pink d) Orange How do eosinophils appear under Giemsa stain? a) Blue-purple nucleus and orange-red granules✔ b) Purple nucleus and pink cytoplasm c) Dark blue nucleus and light blue cytoplasm d) Purple granules How do neutrophils appear under Giemsa stain? a) Purple-red nucleus and pink cytoplasm✔ b) Blue-purple nucleus and orange-red granules c) Dark blue nucleus and light blue cytoplasm d) Purple granules What color do platelets appear after Giemsa staining? a) Pink

Blue

10 / 30

Category: Clinical Laboratory

How are thick smears rinsed after staining?

Washed with tap water

Left in buffer for 5 minutes

Dipped 3-4 times in buffer

Rinsed with methanol

11 / 30

Category: Clinical Laboratory

How do eosinophils appear under Giemsa stain?

Purple granules

Purple nucleus and pink cytoplasm

Dark blue nucleus and light blue cytoplasm

Blue-purple nucleus and orange-red granules

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Category: Clinical Laboratory

Which organism can Giemsa stain NOT identify?

Escherichia coli

Chlamydia trachomatis

Malaria parasites

Histoplasma spp.

13 / 30

Category: Clinical Laboratory

What is the purpose of Giemsa stain in histopathology?

To detect viruses

To stain plant tissues

To identify blood parasites and cellular structures

To stain fungal hyphae

14 / 30

Category: Clinical Laboratory

What is the role of Giemsa stain in Wolbach’s tissue staining?

To stain hematopoietic tissue

To identify viruses

To stain plant roots

To detect fungal spores

15 / 30

Category: Clinical Laboratory

Which of the following is NOT a Romanowsky stain?

Gram stain

Wright stain

Leishman stain

Giemsa stain

16 / 30

Category: Clinical Laboratory

How long should Giemsa stock solution stand before use?

1-2 weeks

6 months

1-2 days

1-2 months

17 / 30

Category: Clinical Laboratory

What is the primary advantage of using Wright-Giemsa stain?

It does not require buffering

It is cheaper

It is faster than Giemsa stain

It provides better results for blood parasites

18 / 30

Category: Clinical Laboratory

What color do platelets appear after Giemsa staining?

Pink

Red

Purple granules

Blue

19 / 30

Category: Clinical Laboratory

What personal protective equipment is required for Giemsa staining?

No special equipment is needed

Gloves, safety glasses, and lab coat

Respirator and boots

Face shield and apron

20 / 30

Category: Clinical Laboratory

How do neutrophils appear under Giemsa stain?

Purple granules

Blue-purple nucleus and orange-red granules

Purple-red nucleus and pink cytoplasm

Dark blue nucleus and light blue cytoplasm

21 / 30

Category: Clinical Laboratory

Who developed Giemsa stain?

A German chemist

Louis Pasteur

Alexander Fleming

Robert Koch

22 / 30

Category: Clinical Laboratory

What is Giemsa banding (G-banding) used for?

Staining bacteria

Staining viral particles

Diagnosing fungal infections

Identifying chromosomal anomalies

23 / 30

Category: Clinical Laboratory

What is the purpose of leaving duplicate smears unstained?

To avoid contamination

To reduce staining time

To serve as backups or for reference labs

To save staining reagents

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Category: Clinical Laboratory

Which reagent is used to fix the blood smear in Giemsa staining?

Eosin

Glycerin

Methanol

Azure

25 / 30

Category: Clinical Laboratory

What is the purpose of Triton X-100 in Giemsa staining?

To evenly distribute the stain

To decolorize the slide

To fix the smear

To act as a buffer

26 / 30

Category: Clinical Laboratory

Why is Giemsa stain stored in a brown bottle?

To maintain its pH

To protect it from light degradation

To keep it sterile

To prevent evaporation

27 / 30

Category: Clinical Laboratory

What is the role of glycerin in Giemsa stain?

Fixative✔

Stabilizer and solvent

Decolorizer

Buffer

28 / 30

Category: Clinical Laboratory

What is the concentration of the working solution for rapid staining?

20%

10%

29 / 30

Category: Clinical Laboratory

How long is a thin blood film stained with Giemsa?

5-10 minutes

45-60 minutes

2 hours

20-30 minutes

30 / 30

Category: Clinical Laboratory

What color is the nucleus of blood cells after Giemsa staining?

Pink

Orange

Red

Blue-purple

Your score is

The average score is 64%

Read Full Article: Giemsa Stain FAQs and MCQs 50 FAQ and 30 MCQs

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