CIN Agar MCQs Mock Test

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Challenge your diagnostic skills with our CIN Agar (Cefsulodin-Irgasan-Novobiocin) Mock Test! This 30-question quiz evaluates your ability to:

✔ Identify Yersinia enterocolitica – Recognize classic “bull’s-eye” colonies
✔ Differentiate Enteric Pathogens – Distinguish Yersinia from Proteus, Citrobacter, and normal flora
✔ Follow CLSI Protocols – Master incubation (32°C), interpretation, and QC
✔ Troubleshoot Common Issues – Address overgrowth, contamination, and atypical morphology

🔬 Key Features:
✓ Image-Based Questions – Analyze real CIN agar plates
✓ Detailed Explanations – Learn while testing
✓ Instant Scoring – Benchmark against peers
✓ Mobile-Optimized – Study anytime, anywhere

🎯 Perfect For:

Medical lab technicians (MLT/MLS)
Microbiology students (ASCP, USMLE prep)
Public health professionals

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Microbiology Department

CIN Agar MCQs Mock Test

Challenge your diagnostic skills with our CIN Agar (Cefsulodin-Irgasan-Novobiocin) Mock Test! This 30-question quiz evaluates your ability to:

🎯 Perfect For:

Medical lab technicians (MLT/MLS)
Microbiology students (ASCP, USMLE prep)
Public health professionals

Read Full Article: Transport Media FAQs and MCQs

1 / 30

Category: Clinical Laboratory

Which test is needed to confirm Y. enterocolitica after CIN Agar?

Urease test

Catalase test

Coagulase test

Oxidase test

2 / 30

Category: Clinical Laboratory

Which agar is not selective for Yersinia?

CIN Agar

Modified CIN Agar

YSA (Yersinia Selective Agar)

Hektoen Enteric Agar

3 / 30

Category: Clinical Laboratory

Which selective agent targets Gram-positive bacteria in CIN Agar?

Crystal violet

Cefsulodin

Neutral red

Sodium pyruvate

4 / 30

Category: Clinical Laboratory

If Proteus mirabilis grows on CIN Agar, what is the likely issue?

Over-autoclaving

CTN supplement failure

All of the above

Incorrect incubation temperature

5 / 30

Category: Clinical Laboratory

What modern technique complements CIN Agar for Yersinia detection?

Wet mount microscopy

MALDI-TOF MS

None

Gram staining

6 / 30

Category: Clinical Laboratory

Which non-Yersinia organism may grow on CIN Agar if cefsulodin is reduced?

Aeromonas spp.

Clostridium difficile

Candida albicans

Mycobacterium tuberculosis

7 / 30

Category: Clinical Laboratory

A key limitation of CIN Agar is:

It kills Yersinia

Turns black with Salmonella

Requires PCR for confirmation

Only works for viral cultures

8 / 30

Category: Clinical Laboratory

The “bull’s-eye” appearance of Y. enterocolitica colonies is due to:

Capsule production

Hemolysis

Mannitol fermentation

Spore formation

9 / 30

Category: Clinical Laboratory

Which organism is not inhibited by CIN Agar?

Staphylococcus aureus

Enterococcus faecalis

Yersinia enterocolitica

Escherichia coli

10 / 30

Category: Clinical Laboratory

What is the final pH of CIN Agar?

8.0 ± 0.2

5.4 ± 0.2

7.4 ± 0.2

6.2 ± 0.2

11 / 30

Category: Clinical Laboratory

Which Yersinia species may produce false-positive results?

Y. pseudotuberculosis

Y. pestis

Y. ruckeri

All of the above

12 / 30

Category: Clinical Laboratory

How does CIN Agar differ from MacConkey Agar?

CIN uses blood; MacConkey uses bile salts

CIN is for anaerobes; MacConkey is for aerobes

CIN selects for Yersinia; MacConkey selects for enterics

No difference

13 / 30

Category: Clinical Laboratory

Which component stimulates Yersinia growth in CIN Agar?

Sodium chloride

Sodium pyruvate

Magnesium sulfate

All of the above

14 / 30

Category: Clinical Laboratory

What color indicates proper CIN Agar preparation?

Black

Green

Blue

Orange-red

15 / 30

Category: Clinical Laboratory

Who first developed CIN Agar?

Schiemann D.A.

Robert Koch

Louis Pasteur

Alexander Fleming

16 / 30

Category: Clinical Laboratory

What is the role of irgasan (triclosan)?

Suppress Gram-positive and Gram-negative bacteria

Stabilize pH

Enhance Yersinia pigment

Inhibit fungi

17 / 30

Category: Clinical Laboratory

Which temperature range is optimal for incubating CIN Agar?

15–20°C

35–37°C

22–32°C

42–45°C

18 / 30

Category: Clinical Laboratory

How should used CIN Agar plates be discarded?

Freezing

Autoclave/incinerate

Direct landfill

Ethanol washing

19 / 30

Category: Clinical Laboratory

What indicates CIN Agar preparation failure?

Cloudy medium before inoculation

All of the above

Growth of E. coli ATCC 25922

Pink color post-solidification

20 / 30

Category: Clinical Laboratory

25. How should CTN supplement be added to CIN Agar base?

At 45°C post-autoclaving

At 60°C

Before autoclaving

After solidification

21 / 30

Category: Clinical Laboratory

Which pH indicator is used in CIN Agar?

Phenol red

Bromothymol blue

Neutral red

Methyl red

22 / 30

Category: Clinical Laboratory

Why are cefsulodin and novobiocin added post-autoclaving?

To prevent agar solidification

They are heat-labile antibiotics

To reduce toxicity

To enhance color change

23 / 30

Category: Clinical Laboratory

How long can prepared CIN Agar be stored at 2–8°C?

1 year

6 months

1 week

3–8 weeks

24 / 30

Category: Clinical Laboratory

What is the primary purpose of CIN Agar?

Selectively grow Yersinia enterocolitica

Isolate Salmonella spp.

Culture anaerobic bacteria

Enrich Escherichia coli

25 / 30

Category: Clinical Laboratory

A fecal sample from a food poisoning outbreak shows bull’s-eye colonies on CIN Agar. The most likely pathogen is:

Yersinia enterocolitica

Vibrio cholerae

Shigella

Salmonella

26 / 30

Category: Clinical Laboratory

29. CIN Agar is used to test which food for Yersinia?

Canned vegetables

Pasteurized milk

All of the above

Undercooked pork

27 / 30

Category: Clinical Laboratory

16. Which component acts as a carbon source in CIN Agar?

Magnesium sulfate

Neutral red

Mannitol

Sodium deoxycholate

28 / 30

Category: Clinical Laboratory

CIN Agar is primarily used for which specimen type?

Sputum

CSF

Fecal samples

Blood cultures

29 / 30

Category: Clinical Laboratory

Which QC organism should show growth with “bull’s-eye” colonies?

E. coli ATCC 25922

P. aeruginosa ATCC 27853

Y. enterocolitica ATCC 27729

S. aureus ATCC 25923

30 / 30

Category: Clinical Laboratory

The acronym “CIN” stands for:

Cefsulodin-Irgasan-Novobiocin

Chloramphenicol-Isoniazid-Nitrofurantoin

Colistin-Irgasan-Nalidixic acid

Ciprofloxacin-Isoniazid-Novobiocin

Your score is

The average score is 65%

Read Full Article: CIN Agar FAQs and MCQs

“How to read CIN agar for Yersinia“
“CIN agar colony morphology MCQs”
“Best temperature for Yersinia culture”
“CLSI guidelines for stool pathogen isolation”

Possible References Used

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