Biochemical Test of Bordetella Pertussis 30 MCQs Mock Test:

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Microbiology Department

Biochemical Test of Bordetella Pertussis MCQs Mock Test

Please read before starting the Biochemical Test of Bordetella Pertussis – 30 MCQs Mock Test. This test includes 30 multiple-choice questions, each with one correct answer. There is no negative marking, so attempt all questions. Avoid refreshing or closing the page to prevent losing your progress. After answering all questions, you may review your responses before submitting. If you want a Certificate, enter your correct Full Name and active Email after completing the test and before submission. Your certificate can be downloaded directly from the result page once the test is submitted.

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Read Full Article: Biochemical Test of Bordetella Pertussis FAQs and MCQs

1 / 30

Category: ASCP Exam Questions

For optimal recovery of Bordetella pertussis, nasopharyngeal swabs should be made of:

Rayon or Dacron

Cotton

Calcium alginate

Wooden stick swabs

Bordetella pertussis (the causative agent of whooping cough) is fastidious and requires specific swab materials for optimal recovery:

1. Rayon or Dacron swabs:

   • Non-inhibitory to bacterial growth (unlike cotton or calcium alginate, which may contain fatty acids or residues that kill B. pertussis).

   • Compatible with PCR and culture (no interference with molecular tests).

2. Nasopharyngeal (NP) swab placement:

   • Must reach the ciliated epithelium (posterior nasopharynx) where B. pertussis colonizes.

Why Not the Other Options?

• a) Cotton swabs → May contain fatty acids that inhibit B. pertussis.

• c) Calcium alginate → Can be toxic to Bordetella and interfere with PCR.

• d) Wooden stick swabs → Not recommended (wood may contain growth inhibitors).

2 / 30

Category: Clinical Laboratory

B. pertussis hydrolyzes gelatin:

Slowly

Rapidly

Only at 37°C

Does not hydrolyze

3 / 30

Category: Clinical Laboratory

Alkaline phosphatase production is:

Inducible

Strongly positive

Biphasic

Negative

4 / 30

Category: Clinical Laboratory

The oxidase test for B. pertussis is:

Positive

Variable

Weakly positive

Negative

5 / 30

Category: Clinical Laboratory

B. pertussis fermentation of xylose is:

Positive

Acidic

Negative

Delayed

6 / 30

Category: Clinical Laboratory

Hemolysis in B. pertussis suggests it can:

Ferment hemoglobin

Survive intracellularly

Lyse red blood cells

Resist antibiotics

7 / 30

Category: Clinical Laboratory

What hemolytic activity does B. pertussis exhibit?

Positive (β-hemolysis)

Negative (no hemolysis)

Gamma-hemolysis

Alpha-hemolysis

8 / 30

Category: Clinical Laboratory

Ester C8 lipase activity is:

Weak

pH-dependent

Negative

Positive

9 / 30

Category: Clinical Laboratory

All of the following are true for B. pertussis EXCEPT:

Oxidase-positive

Non-motile

Glucose fermenter

Catalase-positive

10 / 30

Category: Clinical Laboratory

Chymotrypsin activity in B. pertussis is:

Variable

Not tested

Positive

Negative

11 / 30

Category: Clinical Laboratory

The capsule of B. pertussis is a virulence factor because it:

Enhances motility

Facilitates spore formation

Produces toxins

Evades phagocytosis

12 / 30

Category: Clinical Laboratory

B. pertussis ferments mannitol:

Requires cysteine

Only under aerobic conditions

Yes

No

13 / 30

Category: Clinical Laboratory

Unlike B. bronchiseptica, B. pertussis is:

Urease-positive

Spore-forming

Motile

Non-motile

14 / 30

Category: Clinical Laboratory

B. pertussis possesses:

Flagella

Capsule

Pili

Spores

15 / 30

Category: Clinical Laboratory

What is the Gram stain result for B. pertussis?

Acid-fast

Gram-negative

Gram-variable

Gram-positive

16 / 30

Category: Clinical Laboratory

Urease production in B. pertussis is:

Delayed positive

Strongly positive

Weakly positive

Negative

17 / 30

Category: Clinical Laboratory

Naphthol-AS-B1 phosphohydrolase activity is:

Indeterminate

Strain-specific

Negative

Positive

18 / 30

Category: Clinical Laboratory

B. pertussis grows in nutrient broth:

Only with blood supplements

Slowly after 48 hours

Abundantly

Does not grow

19 / 30

Category: Clinical Laboratory

A key test to differentiate B. pertussis from enteric bacteria is its:

Urease production

Oxidase positivity

Citrate utilization

Lactose fermentation

20 / 30

Category: Clinical Laboratory

The shape of B. pertussis is best described as:

Spirochetes

Minute coccobacilli

Streptobacilli

Diplococci

21 / 30

Category: Clinical Laboratory

Sucrose fermentation is:

Positive

Negative

Weak

Gas-producing

22 / 30

Category: Clinical Laboratory

Both B. pertussis and Haemophilus influenzae are:

Gram-negative coccobacilli

Maltose fermenters

Urease-positive

Spore-forming

23 / 30

Category: Clinical Laboratory

B. pertussis is citrate:

Utilization negative

Variable

Utilization positive

Slow utilizer

24 / 30

Category: Clinical Laboratory

Lysine decarboxylase activity is:

Negative

Weakly positive

Temperature-dependent

Strongly positive

25 / 30

Category: Clinical Laboratory

Nitrate reduction by B. pertussis is:

Negative

Enhanced by glucose

Positive

Requires anaerobic conditions

26 / 30

Category: Clinical Laboratory

Which test helps rule out Pseudomonas?

Nitrate reduction

Oxidase positivity

Catalase positivity

Absence of pigment

27 / 30

Category: Clinical Laboratory

Esculin hydrolysis by B. pertussis is:

Negative

Positive

Variable

Requires light

28 / 30

Category: Clinical Laboratory

A key enzymatic marker for B. pertussis identification is:

Lipase C14 activity

Tryptophanase

DNase

Ester C8 lipase activity

29 / 30

Category: Clinical Laboratory

Which carbon source is fermented by B. pertussis?

Maltose

Sucrose

Glucose

None of the above

30 / 30

Category: Clinical Laboratory

Which motility trait applies to B. pertussis?

Motile via flagella

Non-motile

Twitching motility

Gliding motility

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