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25-Hydroxy Vitamin D Test

Also Known As:  Ergocalciferol (Vitamin D2)Cholecalciferol (Vitamin D3)Calcidiol (25-hydroxyvitamin D)Calcifidiol (25-hydroxy-vitamin D)Calcitriol (1,25 dihydroxy-vitamin D), 25-hydroxyvitamin D1,25 dihydroxyvitamin D, Vitamin D

Test panel: Vitamin D, Vitamin A, Vitamin B, Vitamin B6, Vitamin B12, Vitamin C, Vitamin E, Vitamin K, Calcium

Why Det Tested:

  • Indicated in the postmenopausal women to evaluate Vit.D level which is needed for the absorption of Calcium.
  • To evaluate the role of Vit.D in osteoporosis.
  • To find the role of Vit.D in the prevention of cancer.

When To Get Tested:

Required Sample

  1. Venous blood is needed to prepare the serum.
  2. The random sample can be used.
  3. The sample is stable at room temperature for 72 hours.
    1. The sample is stable at 22 to 25 °C.
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People at risk of Vit.D deficiency are:

Normal Values:

  • Indicate vit.D deficiency = <20 ng/mL 
  •  Insufficiency of vit.D = 20 to 30 ng/mL
  •  Optimal level of vit.D = >30 ng/mL
  • Toxic level of vit.D = >200 ng/mL
    • Recommended dose of vitamin D for adult is 5 µg/day.

Increased level of Vit.D in Seen in:

Decreased level of Vit.D is Seen in :



Possible References Used


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