Learn PAS staining, a fundamental histochemical technique for detecting glycogen, mucins, and fungal organisms in tissue samples. This comprehensive guide covers:
✓ Chemical Principle:
- Periodic acid oxidation of carbohydrates
- Schiff reagent reaction to produce magenta color
✓ Key Diagnostic Uses:
- Fungal identification (Candida, Aspergillus)
- Renal pathology (basement membrane thickening)
- Glycogen storage diseases
- Adenocarcinomas (mucin detection)
✓ Protocol Variations:
- PAS with diastase digestion (glycogen confirmation)
- Combined PAS-Alcian blue for mucin typing
✓ Interpretation Guide:
- Intensity scoring (0 to 4+)
- Common artifacts and troubleshooting
Essential for histotechnologists, pathologists, and microbiology labs working in anatomic pathology and fungal diagnostics.
Challenge your knowledge with 40+ PAS staining MCQs covering:
◼️ Fungal element identification (hyphae vs. yeast forms)
◼️ Renal biopsy interpretation (diabetic nephropathy)
◼️ Glycogen vs. mucin differentiation
◼️ PAS-D technique applications
Perfect for:
- Pathology board exams (USMLE, FRCPath)
- Histotechnology certification (HT/HTL)
- Medical mycology training
Read Full Article: Periodic Acid-Schiff (PAS) Staining 50 FAQ and 30 MCQs






