Papanicolaou Staining (Pap stain) MCQs Mock Test (30 MCQs)

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Master the Papanicolaou (Pap) stain, the gold-standard technique for cervical cancer screening and cytological diagnostics. This comprehensive guide…

Master the Papanicolaou (Pap) stain, the gold-standard technique for cervical cancer screening and cytological diagnostics. This comprehensive guide covers:

✓ Nuclear & cytoplasmic staining principle (Harris hematoxylin, OG-6, EA-50)
✓ Step-by-step protocol for liquid-based vs. conventional smears
✓ Diagnostic applications:

  • Cervical intraepithelial neoplasia (CIN) grading
  • Hormonal evaluation (maturation index)
  • Non-gynecological cytology (urine, sputum)

✓ Quality control measures:

  • Stain filtration schedule
  • pH monitoring of alcohols
  • Troubleshooting common artifacts

Essential for cytotechnologists, pathologists, and screening program technicians.

Test your cytology expertise with 40+ Pap staining MCQs:
◼️ Nuclear/cytoplasmic color interpretation
◼️ Bethesda System correlation
◼️ Liquid-based vs. conventional smear differences
◼️ HSIL vs. LSIL cytomorphology

Ideal for:

  • ASCP cytotechnology certification
  • Pathology residency exams
  • National screening program training
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Cytology and Histology Department

Papanicolaou Staining (Pap stain)  MCQs Mock Test

Papanicolaou Staining (Pap stain) MCQs Mock Test

Test your cytology expertise with 30 Pap staining MCQs:
◼️ Nuclear/cytoplasmic color interpretation
◼️ Bethesda System correlation
◼️ Liquid-based vs. conventional smear differences
◼️ HSIL vs. LSIL cytomorphology

Ideal for:

  • ASCP cytotechnology certification

  • Pathology residency exams

  • National screening program training

Read Full Article: Papanicolaou Staining (Pap stain)  FAQs And MCQs

1 / 30

Category: Clinical Laboratory

What is the main limitation of Pap staining?

2 / 30

Category: Clinical Laboratory

 What is the purpose of phosphotungstic acid in EA?

3 / 30

Category: Clinical Laboratory

How long should a smear be fixed in 95% ethanol for Pap staining?

4 / 30

Category: Clinical Laboratory

 Which of the following is NOT a specimen used for Pap staining?

5 / 30

Category: Clinical Laboratory

Which of these dyes is often omitted in modern Pap stain formulations?

6 / 30

Category: Clinical Laboratory

Which component is NOT part of EA (Eosin Azure)?

7 / 30

Category: Clinical Laboratory

What color do cell nuclei appear after Pap staining?

8 / 30

Category: Clinical Laboratory

 What does poor nuclear staining indicate in Pap smears?

9 / 30

Category: Clinical Laboratory

What is the role of glacial acetic acid in Pap staining?

10 / 30

Category: Clinical Laboratory

 What is the most common fixative for Pap smears?

11 / 30

Category: Clinical Laboratory

What is the role of xylene in Pap staining?

12 / 30

Category: Clinical Laboratory

 What happens if a Pap smear dries before fixation?

13 / 30

Category: Clinical Laboratory

 How are metaplastic cells identified in Pap staining?

14 / 30

Category: Clinical Laboratory

Which of these is NOT a use of Pap staining?

15 / 30

Category: Clinical Laboratory

5. Which component does Orange G-6 primarily stain?

16 / 30

Category: Clinical Laboratory

What color do superficial squamous cells appear after Pap staining?

17 / 30

Category: Clinical Laboratory

 What is the primary purpose of Pap staining?

18 / 30

Category: Clinical Laboratory

 Which dye is responsible for staining RBCs orange-red?

19 / 30

Category: Clinical Laboratory

 Which stain is used for non-gynecological specimens like sputum?

20 / 30

Category: Clinical Laboratory

Who developed the Papanicolaou staining technique?

21 / 30

Category: Clinical Laboratory

Which of these is a counterstain in Pap staining?

22 / 30

Category: Clinical Laboratory

What is the purpose of Scott’s tap water in Pap staining?

23 / 30

Category: Clinical Laboratory

6. What does EA (Eosin Azure) stain in Pap smears?

24 / 30

Category: Clinical Laboratory

 What is the advantage of Pap staining over simple H&E?

25 / 30

Category: Clinical Laboratory

What color do intermediate squamous cells stain?

26 / 30

Category: Clinical Laboratory

 How are Candida species identified in a Pap smear?

27 / 30

Category: Clinical Laboratory

Which of the following is a rapid Pap staining method?

28 / 30

Category: Clinical Laboratory

 Which dye is used as a nuclear stain in Pap staining?

29 / 30

Category: Clinical Laboratory

What is the final step in Pap staining before microscopy?

30 / 30

Category: Clinical Laboratory

What is the most critical step before Pap staining?

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Read Full Article: Papanicolaou Staining (Pap stain)  FAQs And MCQs

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