Negative staining of Viruses MCQs Mock Test (30 MCQs)

Prepare for your virology and microbiology exams with our 30 Negative Staining of Viruses MCQs Mock Test on LabTestsGuide.com! This quiz is designed to test your knowledge of electron microscopy (EM) techniques used in virus visualization, including:

Principle of negative staining (phosphotungstic acid, uranyl acetate)
Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)
Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)
Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications.

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Negative Staining of Viruses MCQs Mock Test

- Principle of negative staining (phosphotungstic acid, uranyl acetate)

- Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)

- Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)

- Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications

Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

Which virus is best studied with ammonium molybdate staining?

Hepatitis B

Influenza (Orthomyxovirus)

Rabies

HIV

2 / 30

Category: Clinical Laboratory

What is a major problem during air-drying of enveloped viruses?

They dissolve in the stain

They multiply rapidly

They flatten and lose structure

They become positively stained

3 / 30

Category: Clinical Laboratory

What does orthomyxovirus (e.g., influenza) show in negative staining?

Spiked surface (hemagglutinin)

Flagella

Thick cell wall

Helical nucleic acid

4 / 30

Category: Clinical Laboratory

What is the primary purpose of negative staining in electron microscopy?

To stain the virus itself

To increase virus infectivity

To stain the background while leaving the virus unstained

To fix the viral nucleic acids

5 / 30

Category: Clinical Laboratory

What artifact can occur due to superimposed staining layers?

Acid-fast staining

Fluorescence quenching

Moiré pattern

Gram-positive reaction

6 / 30

Category: Clinical Laboratory

What is the ideal concentration range for most viruses in negative staining?

10–20 mg/ml

1–5 mg/ml

50–100 mg/ml

0.05–0.1 mg/ml

7 / 30

Category: Clinical Laboratory

How long should uranyl acetate be stirred before use?

24 hours

30 minutes to 1 hour

1–2 minutes

Not required

8 / 30

Category: Clinical Laboratory

Why is ammonium molybdate preferred for large viruses?

It binds tightly to the support film

It stains nucleic acids

It is radioactive

It works only at high pH

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Category: Clinical Laboratory

What is a disadvantage of uranyl acetate?

It cannot be filtered

It can be radioactive

It produces low contrast

It only works for non-enveloped viruses

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Category: Clinical Laboratory

Which of the following is NOT an application of negative staining?

Visualizing bacterial flagella

Sequencing viral RNA

Studying viral morphology

Diagnosing bacterial infections

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Category: Clinical Laboratory

How does negative staining create contrast in TEM images?

By absorbing electrons in the stained background

By amplifying viral RNA

By using antibodies

By staining the virus directly

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Category: Clinical Laboratory

In negative staining, how do viruses appear under TEM?

Invisible

Dark against a light background

Light against a dark background

Fluorescent green

13 / 30

Category: Clinical Laboratory

What is a limitation of negative staining?

It requires live viruses

It can distort fragile viruses

It only works for fungi

It cannot be used with TEM

14 / 30

Category: Clinical Laboratory

Who first introduced negative staining?

Robert Koch

Brenner and Horne (1959)

Antonie van Leeuwenhoek

Louis Pasteur

15 / 30

Category: Clinical Laboratory

15. What is used to remove excess stain from the grid?

Filter paper wicking

Heat fixation

Centrifugation

Alcohol wash

16 / 30

Category: Clinical Laboratory

Why might some viruses appear “collapsed” in TEM?

Due to freeze-thawing

Due to one-sided staining

Because of high pH

Due to over-staining

17 / 30

Category: Clinical Laboratory

Which stain is chemically inert and has a neutral pH?

Methylene blue

Crystal violet

Sodium silicotungstate

Uranyl acetate

18 / 30

Category: Clinical Laboratory

Which stain is commonly used for enveloped viruses due to its low pH?

Phosphotungstic acid

Sodium silicotungstate (SST)

Ammonium molybdate

Uranyl acetate

19 / 30

Category: Clinical Laboratory

What happens if too much stain is left on the grid?

The virus disintegrates

The virus appears brighter

The grid becomes hydrophobic

Only stain is visible, obscuring the virus

20 / 30

Category: Clinical Laboratory

Which microscope is most commonly used for negative staining of viruses?

Fluorescence microscope

Scanning Electron Microscope (SEM)

Transmission Electron Microscope (TEM)

Light microscope

21 / 30

Category: Clinical Laboratory

What is the pH of uranyl acetate?

9.2 (basic)

4.4 (acidic)

7.0 (neutral)

2.0 (highly acidic)

22 / 30

Category: Clinical Laboratory

Why is negative staining disappearing from mainstream use?

It cannot stain bacteria

It is too expensive

Advanced techniques (e.g., cryo-EM) offer better resolution

It requires radioactive stains

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Category: Clinical Laboratory

What viral feature is commonly observed in adenovirus after negative staining?

Capsid symmetry

Lipid blebs

Double-stranded DNA

Long fibers and penton bases

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Category: Clinical Laboratory

What type of stains are used in negative staining?

Heavy metal salts (e.g., uranium, tungsten)

Basic dyes

Methylene blue

Fluorescent dyes

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Category: Clinical Laboratory

Negative staining is most useful for:

Quantifying viral load

Culturing viruses

Rapid preliminary virus identification

DNA extraction

26 / 30

Category: Clinical Laboratory

What is “immuno-negative staining”?

A fluorescence technique

A type of Gram staining

A DNA sequencing method

Combining antibodies with negative staining

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Category: Clinical Laboratory

Which support film is preferred for negative staining?

Plastic film

Carbon film

Glass slide

Gold film

28 / 30

Category: Clinical Laboratory

Why is carbon film preferred over plastic film?

It fluoresces under UV

It is cheaper

It provides better adsorption and contrast

It is hydrophobic

29 / 30

Category: Clinical Laboratory

Which safety precaution is necessary when handling uranyl acetate?

Avoiding water contact

Wearing gloves and a lab coat

Using a fume hood

Keeping it frozen

30 / 30

Category: Clinical Laboratory

Negative staining is particularly useful for studying:

Only bacterial cells

Fungal spores

Only plant tissues

Viruses and bacterial surface structures

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Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

“Best MCQs on negative staining for TEM”
“How to identify virus morphology using EM”
“Phosphotungstic acid vs. uranyl acetate staining”
“Electron microscopy virus detection quiz”

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