Negative staining of Viruses MCQs Mock Test (30 MCQs)

Prepare for your virology and microbiology exams with our 30 Negative Staining of Viruses MCQs Mock Test on LabTestsGuide.com! This quiz is designed to test your knowledge of electron microscopy (EM) techniques used in virus visualization, including:

Principle of negative staining (phosphotungstic acid, uranyl acetate)
Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)
Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)
Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications.

0 votes, 0 avg

Hematology Department

Negative Staining of Viruses MCQs Mock Test

- Principle of negative staining (phosphotungstic acid, uranyl acetate)

- Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)

- Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)

- Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications

Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

Why is carbon film preferred over plastic film?

It provides better adsorption and contrast

It fluoresces under UV

It is cheaper

It is hydrophobic

2 / 30

Category: Clinical Laboratory

How does negative staining create contrast in TEM images?

By staining the virus directly

By amplifying viral RNA

By using antibodies

By absorbing electrons in the stained background

3 / 30

Category: Clinical Laboratory

Which stain is chemically inert and has a neutral pH?

Methylene blue

Sodium silicotungstate

Uranyl acetate

Crystal violet

4 / 30

Category: Clinical Laboratory

Which support film is preferred for negative staining?

Gold film

Carbon film

Glass slide

Plastic film

5 / 30

Category: Clinical Laboratory

Which virus is best studied with ammonium molybdate staining?

Rabies

Influenza (Orthomyxovirus)

HIV

Hepatitis B

6 / 30

Category: Clinical Laboratory

In negative staining, how do viruses appear under TEM?

Dark against a light background

Invisible

Fluorescent green

Light against a dark background

7 / 30

Category: Clinical Laboratory

Which safety precaution is necessary when handling uranyl acetate?

Using a fume hood

Avoiding water contact

Keeping it frozen

Wearing gloves and a lab coat

8 / 30

Category: Clinical Laboratory

What does orthomyxovirus (e.g., influenza) show in negative staining?

Thick cell wall

Spiked surface (hemagglutinin)

Helical nucleic acid

Flagella

9 / 30

Category: Clinical Laboratory

What is a major problem during air-drying of enveloped viruses?

They dissolve in the stain

They multiply rapidly

They flatten and lose structure

They become positively stained

10 / 30

Category: Clinical Laboratory

What type of stains are used in negative staining?

Fluorescent dyes

Heavy metal salts (e.g., uranium, tungsten)

Basic dyes

Methylene blue

11 / 30

Category: Clinical Laboratory

Negative staining is particularly useful for studying:

Only plant tissues

Viruses and bacterial surface structures

Fungal spores

Only bacterial cells

12 / 30

Category: Clinical Laboratory

Which stain is commonly used for enveloped viruses due to its low pH?

Uranyl acetate

Sodium silicotungstate (SST)

Ammonium molybdate

Phosphotungstic acid

13 / 30

Category: Clinical Laboratory

What viral feature is commonly observed in adenovirus after negative staining?

Lipid blebs

Capsid symmetry

Long fibers and penton bases

Double-stranded DNA

14 / 30

Category: Clinical Laboratory

What happens if too much stain is left on the grid?

The virus appears brighter

The virus disintegrates

The grid becomes hydrophobic

Only stain is visible, obscuring the virus

15 / 30

Category: Clinical Laboratory

What is a disadvantage of uranyl acetate?

It cannot be filtered

It only works for non-enveloped viruses

It produces low contrast

It can be radioactive

16 / 30

Category: Clinical Laboratory

Why is ammonium molybdate preferred for large viruses?

It works only at high pH

It is radioactive

It binds tightly to the support film

It stains nucleic acids

17 / 30

Category: Clinical Laboratory

What is the ideal concentration range for most viruses in negative staining?

0.05–0.1 mg/ml

10–20 mg/ml

50–100 mg/ml

1–5 mg/ml

18 / 30

Category: Clinical Laboratory

What is the pH of uranyl acetate?

9.2 (basic)

4.4 (acidic)

7.0 (neutral)

2.0 (highly acidic)

19 / 30

Category: Clinical Laboratory

What is “immuno-negative staining”?

A fluorescence technique

Combining antibodies with negative staining

A DNA sequencing method

A type of Gram staining

20 / 30

Category: Clinical Laboratory

Why might some viruses appear “collapsed” in TEM?

Because of high pH

Due to one-sided staining

Due to over-staining

Due to freeze-thawing

21 / 30

Category: Clinical Laboratory

Which of the following is NOT an application of negative staining?

Diagnosing bacterial infections

Sequencing viral RNA

Visualizing bacterial flagella

Studying viral morphology

22 / 30

Category: Clinical Laboratory

What is a limitation of negative staining?

It requires live viruses

It can distort fragile viruses

It only works for fungi

It cannot be used with TEM

23 / 30

Category: Clinical Laboratory

How long should uranyl acetate be stirred before use?

Not required

24 hours

1–2 minutes

30 minutes to 1 hour

24 / 30

Category: Clinical Laboratory

What is the primary purpose of negative staining in electron microscopy?

To stain the virus itself

To stain the background while leaving the virus unstained

To fix the viral nucleic acids

To increase virus infectivity

25 / 30

Category: Clinical Laboratory

Which microscope is most commonly used for negative staining of viruses?

Scanning Electron Microscope (SEM)

Fluorescence microscope

Transmission Electron Microscope (TEM)

Light microscope

26 / 30

Category: Clinical Laboratory

15. What is used to remove excess stain from the grid?

Filter paper wicking

Alcohol wash

Heat fixation

Centrifugation

27 / 30

Category: Clinical Laboratory

Why is negative staining disappearing from mainstream use?

It cannot stain bacteria

It is too expensive

It requires radioactive stains

Advanced techniques (e.g., cryo-EM) offer better resolution

28 / 30

Category: Clinical Laboratory

Negative staining is most useful for:

Quantifying viral load

Culturing viruses

DNA extraction

Rapid preliminary virus identification

29 / 30

Category: Clinical Laboratory

What artifact can occur due to superimposed staining layers?

Moiré pattern

Gram-positive reaction

Acid-fast staining

Fluorescence quenching

30 / 30

Category: Clinical Laboratory

Who first introduced negative staining?

Brenner and Horne (1959)

Antonie van Leeuwenhoek

Louis Pasteur

Robert Koch

If you want to download Certificates then Please Put your Name and Correct Email

Your score is

The average score is 0%

Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

“Best MCQs on negative staining for TEM”
“How to identify virus morphology using EM”
“Phosphotungstic acid vs. uranyl acetate staining”
“Electron microscopy virus detection quiz”

📚 This article may have been prepared using trusted medical literature, clinical guidelines, textbooks, and peer-reviewed sources.

View Possible References Used →

Shares0Share0 Tweet0 Pin0 LinkedIn0 Email0

Report a question

Leave a Reply Cancel reply