Negative staining of Viruses MCQs Mock Test (30 MCQs)

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Prepare for your virology and microbiology exams with our 30 Negative Staining of Viruses MCQs Mock Test on LabTestsGuide.com! This quiz is designed to test your knowledge of electron microscopy (EM) techniques used in virus visualization, including:

Principle of negative staining (phosphotungstic acid, uranyl acetate)
Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)
Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)
Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications.

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Hematology Department

Negative Staining of Viruses MCQs Mock Test

- Principle of negative staining (phosphotungstic acid, uranyl acetate)

- Virus morphology identification (enveloped vs. non-enveloped, capsid symmetry)

- Commonly studied viruses (Herpesviruses, Influenza, Bacteriophages)

- Artifacts and troubleshooting in EM sample preparation

Perfect for medical students, virologists, microbiologists, and lab technicians preparing for board exams, certifications, and research applications

Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

What viral feature is commonly observed in adenovirus after negative staining?

Lipid blebs

Capsid symmetry

Double-stranded DNA

Long fibers and penton bases

2 / 30

Category: Clinical Laboratory

Why is carbon film preferred over plastic film?

It is hydrophobic

It fluoresces under UV

It provides better adsorption and contrast

It is cheaper

3 / 30

Category: Clinical Laboratory

What is the pH of uranyl acetate?

9.2 (basic)

4.4 (acidic)

2.0 (highly acidic)

7.0 (neutral)

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Category: Clinical Laboratory

15. What is used to remove excess stain from the grid?

Heat fixation

Centrifugation

Alcohol wash

Filter paper wicking

5 / 30

Category: Clinical Laboratory

What happens if too much stain is left on the grid?

Only stain is visible, obscuring the virus

The virus disintegrates

The grid becomes hydrophobic

The virus appears brighter

6 / 30

Category: Clinical Laboratory

How long should uranyl acetate be stirred before use?

1–2 minutes

24 hours

Not required

30 minutes to 1 hour

7 / 30

Category: Clinical Laboratory

What is the ideal concentration range for most viruses in negative staining?

10–20 mg/ml

0.05–0.1 mg/ml

50–100 mg/ml

1–5 mg/ml

8 / 30

Category: Clinical Laboratory

What is a limitation of negative staining?

It only works for fungi

It requires live viruses

It cannot be used with TEM

It can distort fragile viruses

9 / 30

Category: Clinical Laboratory

How does negative staining create contrast in TEM images?

By amplifying viral RNA

By absorbing electrons in the stained background

By using antibodies

By staining the virus directly

10 / 30

Category: Clinical Laboratory

Which stain is commonly used for enveloped viruses due to its low pH?

Sodium silicotungstate (SST)

Ammonium molybdate

Phosphotungstic acid

Uranyl acetate

11 / 30

Category: Clinical Laboratory

Which microscope is most commonly used for negative staining of viruses?

Light microscope

Transmission Electron Microscope (TEM)

Scanning Electron Microscope (SEM)

Fluorescence microscope

12 / 30

Category: Clinical Laboratory

What is a disadvantage of uranyl acetate?

It only works for non-enveloped viruses

It cannot be filtered

It can be radioactive

It produces low contrast

13 / 30

Category: Clinical Laboratory

Which virus is best studied with ammonium molybdate staining?

Influenza (Orthomyxovirus)

Rabies

HIV

Hepatitis B

14 / 30

Category: Clinical Laboratory

Which of the following is NOT an application of negative staining?

Studying viral morphology

Diagnosing bacterial infections

Visualizing bacterial flagella

Sequencing viral RNA

15 / 30

Category: Clinical Laboratory

What type of stains are used in negative staining?

Fluorescent dyes

Heavy metal salts (e.g., uranium, tungsten)

Methylene blue

Basic dyes

16 / 30

Category: Clinical Laboratory

What artifact can occur due to superimposed staining layers?

Moiré pattern

Fluorescence quenching

Gram-positive reaction

Acid-fast staining

17 / 30

Category: Clinical Laboratory

Who first introduced negative staining?

Louis Pasteur

Antonie van Leeuwenhoek

Robert Koch

Brenner and Horne (1959)

18 / 30

Category: Clinical Laboratory

Negative staining is most useful for:

Rapid preliminary virus identification

Quantifying viral load

Culturing viruses

DNA extraction

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Category: Clinical Laboratory

In negative staining, how do viruses appear under TEM?

Fluorescent green

Light against a dark background

Dark against a light background

Invisible

20 / 30

Category: Clinical Laboratory

Which support film is preferred for negative staining?

Carbon film

Gold film

Glass slide

Plastic film

21 / 30

Category: Clinical Laboratory

What is the primary purpose of negative staining in electron microscopy?

To stain the virus itself

To fix the viral nucleic acids

To increase virus infectivity

To stain the background while leaving the virus unstained

22 / 30

Category: Clinical Laboratory

Why might some viruses appear “collapsed” in TEM?

Due to one-sided staining

Due to freeze-thawing

Due to over-staining

Because of high pH

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Category: Clinical Laboratory

Negative staining is particularly useful for studying:

Fungal spores

Only bacterial cells

Viruses and bacterial surface structures

Only plant tissues

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Category: Clinical Laboratory

Why is ammonium molybdate preferred for large viruses?

It binds tightly to the support film

It is radioactive

It stains nucleic acids

It works only at high pH

25 / 30

Category: Clinical Laboratory

What is “immuno-negative staining”?

A fluorescence technique

Combining antibodies with negative staining

A DNA sequencing method

A type of Gram staining

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Category: Clinical Laboratory

Why is negative staining disappearing from mainstream use?

It requires radioactive stains

Advanced techniques (e.g., cryo-EM) offer better resolution

It cannot stain bacteria

It is too expensive

27 / 30

Category: Clinical Laboratory

Which stain is chemically inert and has a neutral pH?

Crystal violet

Uranyl acetate

Methylene blue

Sodium silicotungstate

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Category: Clinical Laboratory

What is a major problem during air-drying of enveloped viruses?

They multiply rapidly

They dissolve in the stain

They flatten and lose structure

They become positively stained

29 / 30

Category: Clinical Laboratory

What does orthomyxovirus (e.g., influenza) show in negative staining?

Helical nucleic acid

Thick cell wall

Flagella

Spiked surface (hemagglutinin)

30 / 30

Category: Clinical Laboratory

Which safety precaution is necessary when handling uranyl acetate?

Keeping it frozen

Wearing gloves and a lab coat

Avoiding water contact

Using a fume hood

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Read Full Article: Negative staining of Viruses 50 FAQ and 30 MCQs

“Best MCQs on negative staining for TEM”
“How to identify virus morphology using EM”
“Phosphotungstic acid vs. uranyl acetate staining”
“Electron microscopy virus detection quiz”

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