Lactophenol Cotton Blue (LPCB) Staining MCQs Mock Test (30 MCQs)

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Learn Lactophenol Cotton Blue (LPCB) staining, the gold standard for fungal microscopy and morphological identification. This guide covers:
✓ Principle of LPCB staining for fungal hyphae, spores, and structures
✓ Step-by-step protocol for slide preparation and staining
✓ Key applications in diagnosing dermatophytes, molds, and yeasts
✓ Comparison with KOH mount and India ink techniques

Essential for mycologists, microbiologists, and lab technicians working in clinical mycology and research.

Test your mycology knowledge with 30+ LPCB staining MCQs! This quiz covers:
◼️ Staining mechanism for fungal cell walls
◼️ Identification of Aspergillus, Penicillium, and Candida species
◼️ Troubleshooting common staining artifacts
◼️ Comparison with Calcofluor White and Gram staining

Perfect for medical microbiology exams, lab technician certification, and mycology research.

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Microbiology Department

Lactophenol Cotton Blue (LPCB) Staining MCQs Mock Test

Essential for mycologists, microbiologists, and lab technicians working in clinical mycology and research.

Perfect for medical microbiology exams, lab technician certification, and mycology research.

Read Full Article: Lactophenol Cotton Blue (LPCB) Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

What is the role of glycerol in LPCB stain?

Fixative

Staining agent

Provides a semipermanent preparation

Kills parasites

2 / 30

Category: Clinical Laboratory

What is the role of lactic acid in LPCB for stool samples?

Fixative and killing agent

Staining agent

Solvent

Preservative

3 / 30

Category: Clinical Laboratory

Which of the following is true about the preparation of Lactophenol Blue Stain?

It cannot be stored

It is prepared over two days

It requires heating

It is prepared in one step

4 / 30

Category: Clinical Laboratory

Which of the following is true about LPCB wet mounts?

They cannot be used for stool samples

They are only used for fungal staining

They are temporary mounts

They are permanent mounts

5 / 30

Category: Clinical Laboratory

Which of the following is NOT a step in the preparation of Lactophenol Blue Stain?

Autoclaving the solution

Dissolving cotton blue in distillater

Filtering the solution

Adding phenol crystals to lactic acid ed w

6 / 30

Category: Clinical Laboratory

What is the advantage of LPCB wet mounts over saline wet mounts?

Faster preparation time

Lower cost

Better staining of internal structures

Preservation of motility

7 / 30

Category: Clinical Laboratory

How long does it take to prepare Lactophenol Blue Stain?

2 days

1 week

1 hour

1 day

8 / 30

Category: Clinical Laboratory

Which of the following is used for quality control of Lactophenol Blue Stain?

Saline solution

Distilled water

Known reference organisms

Iodine stain

9 / 30

Category: Clinical Laboratory

Which of the following is a disadvantage of saline wet mounts?

Poor visibility of internal structures

High cost

Toxicity

Long preparation time

10 / 30

Category: Clinical Laboratory

Which procedure is used for preparing a temporary fungal mount?

Ziehl-Neelsen Staining

Tease Mount Procedure

Gram Staining

Acid-Fast Staining

11 / 30

Category: Clinical Laboratory

Which of the following is a limitation of the Scotch Tape Procedure?

It is a temporary mount and will dissolve eventually

It requires a special microscope

It is a permanent mount

It cannot be used for fungi

12 / 30

Category: Clinical Laboratory

What is the role of cotton blue in Lactophenol Blue Stain?

Acts as a fixative

Stains fungal elements

Preserves the slide

Kills bacteria

13 / 30

Category: Clinical Laboratory

What is the role of distilled water in LPCB stain preparation?

To kill parasites

To enhance staining

To act as a fixative

To dissolve cotton blue

14 / 30

Category: Clinical Laboratory

What is the purpose of sealing the coverslip with nail polish?

To preserve the mount as a reference slide

To prevent evaporation

To kill the fungi

To enhance staining

15 / 30

Category: Clinical Laboratory

What is the primary purpose of using Lactophenol Blue Stain in stool examination?

To identify parasites

To detect fungal infections

To stain viruses

To detect bacteria

16 / 30

Category: Clinical Laboratory

Which of the following parasites can be detected using Lactophenol Blue Stain?

Entamoeba histolytica

Hookworm

All of the above

Giardia lamblia

17 / 30

Category: Clinical Laboratory

Which of the following is NOT a fungal preparation method using Lactophenol Blue Stain?

Gram Staining

Slide Culture

Tease Mount

Scotch Tape Procedure

18 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of Lactophenol Blue Stain?

Phenol

Glycerol

Ethanol

Lactic acid

19 / 30

Category: Clinical Laboratory

What is the purpose of quality control for Lactophenol Blue Stain?

To ensure the performance of the stain

To enhance staining

To reduce cost

To speed up preparation

20 / 30

Category: Clinical Laboratory

What is the shelf life of Lactophenol Blue Stain when stored properly?

104 weeks

12 weeks

52 weeks

26 weeks

21 / 30

Category: Clinical Laboratory

What is the purpose of filtering the cotton blue solution?

To enhance staining

To remove insoluble dye particles

To kill bacteria

To preserve the stain

22 / 30

Category: Clinical Laboratory

What is the role of phenol crystals in LPCB stain?

Enhances motility

Fixative and preservative

Staining agent

Solvent

23 / 30

Category: Clinical Laboratory

Which of the following is true about Lactophenol Blue Stain?

It is toxic and cannot be handled safely

It can only be used for pigmented moulds

It is used for both fungal and stool examinations

It is only used for bacterial staining

24 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of LPCB stain?

Glycerol

Ethanol

Lactic acid

Phenol

25 / 30

Category: Clinical Laboratory

What is the role of phenol in Lactophenol Blue Stain?

Staining agent

Preservative

Solvent

Fixative and killing agent

26 / 30

Category: Clinical Laboratory

What is the disadvantage of using Lactophenol Blue Stain for stool samples?

It is toxic to handle

It requires special equipment

It cannot detect parasites

Bile-stained helminthic ova lose their natural color

27 / 30

Category: Clinical Laboratory

What is the recommended storage condition for Lactophenol Blue Stain?

Frozen

Exposed to sunlight

Room temperature, protected from light

Refrigerated

28 / 30

Category: Clinical Laboratory

What is the primary use of Lactophenol Blue Stain?

Mounting and staining fungi

Detecting viruses

Staining bacteria

Staining parasites in blood

29 / 30

Category: Clinical Laboratory

Which of the following is required to prepare Lactophenol Blue Stain?

Incubator

Magnetic stirrer

Centrifuge

Autoclave

30 / 30

Category: Clinical Laboratory

What is the primary advantage of LPCB stain for low-power microscopic screening?

It is faster to prepare

It preserves motility

It is cheaper

It makes parasites more visible

Your score is

The average score is 69%

Read Full Article: Lactophenol Cotton Blue (LPCB) Staining 50 FAQ and 30 MCQs

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