Lactophenol Cotton Blue (LPCB) Staining MCQs Mock Test (30 MCQs)

Learn Lactophenol Cotton Blue (LPCB) staining, the gold standard for fungal microscopy and morphological identification. This guide covers:
✓ Principle of LPCB staining for fungal hyphae, spores, and structures
✓ Step-by-step protocol for slide preparation and staining
✓ Key applications in diagnosing dermatophytes, molds, and yeasts
✓ Comparison with KOH mount and India ink techniques

Essential for mycologists, microbiologists, and lab technicians working in clinical mycology and research.

Test your mycology knowledge with 30+ LPCB staining MCQs! This quiz covers:
◼️ Staining mechanism for fungal cell walls
◼️ Identification of Aspergillus, Penicillium, and Candida species
◼️ Troubleshooting common staining artifacts
◼️ Comparison with Calcofluor White and Gram staining

Perfect for medical microbiology exams, lab technician certification, and mycology research.

0 votes, 0 avg

Microbiology Department

Lactophenol Cotton Blue (LPCB) Staining MCQs Mock Test

Essential for mycologists, microbiologists, and lab technicians working in clinical mycology and research.

Perfect for medical microbiology exams, lab technician certification, and mycology research.

Read Full Article: Lactophenol Cotton Blue (LPCB) Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

Which of the following is used for quality control of Lactophenol Blue Stain?

Saline solution

Iodine stain

Known reference organisms

Distilled water

2 / 30

Category: Clinical Laboratory

Which of the following is NOT a step in the preparation of Lactophenol Blue Stain?

Adding phenol crystals to lactic acid ed w

Dissolving cotton blue in distillater

Filtering the solution

Autoclaving the solution

3 / 30

Category: Clinical Laboratory

What is the primary use of Lactophenol Blue Stain?

Mounting and staining fungi

Staining parasites in blood

Detecting viruses

Staining bacteria

4 / 30

Category: Clinical Laboratory

What is the disadvantage of using Lactophenol Blue Stain for stool samples?

Bile-stained helminthic ova lose their natural color

It is toxic to handle

It requires special equipment

It cannot detect parasites

5 / 30

Category: Clinical Laboratory

How long does it take to prepare Lactophenol Blue Stain?

2 days

1 week

1 hour

1 day

6 / 30

Category: Clinical Laboratory

What is the primary purpose of using Lactophenol Blue Stain in stool examination?

To stain viruses

To identify parasites

To detect fungal infections

To detect bacteria

7 / 30

Category: Clinical Laboratory

Which of the following parasites can be detected using Lactophenol Blue Stain?

Hookworm

Giardia lamblia

Entamoeba histolytica

All of the above

8 / 30

Category: Clinical Laboratory

What is the role of phenol in Lactophenol Blue Stain?

Staining agent

Solvent

Preservative

Fixative and killing agent

9 / 30

Category: Clinical Laboratory

What is the role of lactic acid in LPCB for stool samples?

Staining agent

Fixative and killing agent

Preservative

Solvent

10 / 30

Category: Clinical Laboratory

What is the role of phenol crystals in LPCB stain?

Fixative and preservative

Staining agent

Enhances motility

Solvent

11 / 30

Category: Clinical Laboratory

Which of the following is a limitation of the Scotch Tape Procedure?

It cannot be used for fungi

It is a permanent mount

It requires a special microscope

It is a temporary mount and will dissolve eventually

12 / 30

Category: Clinical Laboratory

What is the recommended storage condition for Lactophenol Blue Stain?

Refrigerated

Frozen

Room temperature, protected from light

Exposed to sunlight

13 / 30

Category: Clinical Laboratory

What is the purpose of sealing the coverslip with nail polish?

To prevent evaporation

To enhance staining

To kill the fungi

To preserve the mount as a reference slide

14 / 30

Category: Clinical Laboratory

Which of the following is NOT a fungal preparation method using Lactophenol Blue Stain?

Gram Staining

Slide Culture

Tease Mount

Scotch Tape Procedure

15 / 30

Category: Clinical Laboratory

Which of the following is true about the preparation of Lactophenol Blue Stain?

It is prepared in one step

It cannot be stored

It requires heating

It is prepared over two days

16 / 30

Category: Clinical Laboratory

Which of the following is a disadvantage of saline wet mounts?

Poor visibility of internal structures

High cost

Long preparation time

Toxicity

17 / 30

Category: Clinical Laboratory

What is the role of glycerol in LPCB stain?

Kills parasites

Provides a semipermanent preparation

Staining agent

Fixative

18 / 30

Category: Clinical Laboratory

What is the purpose of quality control for Lactophenol Blue Stain?

To reduce cost

To ensure the performance of the stain

To enhance staining

To speed up preparation

19 / 30

Category: Clinical Laboratory

What is the primary advantage of LPCB stain for low-power microscopic screening?

It is cheaper

It makes parasites more visible

It is faster to prepare

It preserves motility

20 / 30

Category: Clinical Laboratory

What is the shelf life of Lactophenol Blue Stain when stored properly?

12 weeks

26 weeks

52 weeks

104 weeks

21 / 30

Category: Clinical Laboratory

Which procedure is used for preparing a temporary fungal mount?

Gram Staining

Tease Mount Procedure

Ziehl-Neelsen Staining

Acid-Fast Staining

22 / 30

Category: Clinical Laboratory

Which of the following is required to prepare Lactophenol Blue Stain?

Autoclave

Magnetic stirrer

Incubator

Centrifuge

23 / 30

Category: Clinical Laboratory

What is the role of cotton blue in Lactophenol Blue Stain?

Acts as a fixative

Kills bacteria

Preserves the slide

Stains fungal elements

24 / 30

Category: Clinical Laboratory

What is the purpose of filtering the cotton blue solution?

To remove insoluble dye particles

To enhance staining

To preserve the stain

To kill bacteria

25 / 30

Category: Clinical Laboratory

Which of the following is true about Lactophenol Blue Stain?

It can only be used for pigmented moulds

It is toxic and cannot be handled safely

It is used for both fungal and stool examinations

It is only used for bacterial staining

26 / 30

Category: Clinical Laboratory

What is the advantage of LPCB wet mounts over saline wet mounts?

Faster preparation time

Preservation of motility

Better staining of internal structures

Lower cost

27 / 30

Category: Clinical Laboratory

What is the role of distilled water in LPCB stain preparation?

To dissolve cotton blue

To kill parasites

To act as a fixative

To enhance staining

28 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of Lactophenol Blue Stain?

Phenol

Lactic acid

Ethanol

Glycerol

29 / 30

Category: Clinical Laboratory

Which of the following is true about LPCB wet mounts?

They are only used for fungal staining

They are permanent mounts

They are temporary mounts

They cannot be used for stool samples

30 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of LPCB stain?

Ethanol

Glycerol

Phenol

Lactic acid

If you want to download Certificates then Please Put your Name and Correct Email

Your score is

The average score is 0%

Read Full Article: Lactophenol Cotton Blue (LPCB) Staining 50 FAQ and 30 MCQs

Possible References Used

Shares0Share0 Tweet0 Pin0 LinkedIn0 Email0

2 Comments

Pooja says:
at
I like mock test. It helps to make me comfortable and confident. Thank you
1. Editorial Staff says:
  at
  Happy to hear the mock tests boosted your confidence! Thanks for the kind words. 😊

Lactophenol Cotton Blue (LPCB) Staining MCQs Mock Test (30 MCQs)

Triple Sugar Iron (TSI) Agar

MicroBiology MCQs with Answer and Explanations | Chapter 67

Chapter 101 – MCQs for Lab (Biochemistry)

ketosis

Sabouraud Dextrose Agar (SDA) MCQs Mock Test

Crystals in Urine

2 Comments

Leave a Reply Cancel reply

Report a question

Related Posts

2 Comments

Leave a Reply Cancel reply