Iron-Hematoxylin Staining MCQs Mock Test (30 MCQs)

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Master Iron-Hematoxylin staining, a fundamental histological technique for nuclear staining and parasite identification. This comprehensive guide covers:
✓ Principle behind Weigert’s and Heidenhain’s methods
✓ Step-by-step protocol with troubleshooting tips
✓ Key applications in diagnosing intestinal parasites (e.g., Entamoeba histolytica)
✓ Differences from regular hematoxylin staining

Ideal for histotechnologists, pathologists, and microbiology students preparing for board exams or lab work.

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Microbiology Department

Iron-Hematoxylin Staining MCQs Mock Test

Ideal for histotechnologists, pathologists, and microbiology students preparing for board exams or lab work.

Read Full Article: Iron-Hematoxylin Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

Why is picric acid used in some Iron-Hematoxylin protocols?

To oxidize hematoxylin

To preserve slides

To fix the specimen

To differentiate (destain) excess dye

2 / 30

Category: Clinical Laboratory

Which organism is NOT well-visualized with Iron-Hematoxylin?

Entamoeba histolytica

Ascaris lumbricoides (helminth egg)

Giardia lamblia

Dientamoeba fragilis

3 / 30

Category: Clinical Laboratory

How should protozoan nuclei appear after proper staining?

Bright red

Green-blue

Pale yellow

Dark purple/black

4 / 30

Category: Clinical Laboratory

What is the purpose of Mayer’s albumin in SAF-preserved samples?

To remove mercury

To act as a mordant

To adhere sediment to slides

To oxidize hematoxylin

5 / 30

Category: Clinical Laboratory

Which counterstain pairs well with Iron-Hematoxylin?

Eosin

Methylene blue

Crystal violet

Safranin

6 / 30

Category: Clinical Laboratory

How should PVA-preserved stool samples be prepared for staining?

Freeze before use

Mix with PVA, dry on slides, and fix

Centrifuge and use supernatant

Directly stain without fixation

7 / 30

Category: Clinical Laboratory

Why must PVA smears be dried thoroughly before staining?

To enhance hemoglobin staining

To avoid over-oxidation

To reduce background debris

To prevent cracking

8 / 30

Category: Clinical Laboratory

Which step is unique to SAF-preserved specimens?

Post-fixation with Schaudinn’s

Use of iodine-alcohol

Centrifugation to collect sediment

Staining with carbol fuchsin

9 / 30

Category: Clinical Laboratory

How long should slides remain in Schaudinn’s fixative for optimal results?

30 minutes

Overnight

5 minutes

2 hours

10 / 30

Category: Clinical Laboratory

How long can the Iron-Hematoxylin working solution be stored?

1 day

1 year

1 week

1 month

11 / 30

Category: Clinical Laboratory

Why is Iron-Hematoxylin unsuitable for Isospora belli?

Requires acid-fast stains

Over-stains cytoplasm

Destroys oocyst walls

Oocysts stain poorly

12 / 30

Category: Clinical Laboratory

Which preservative is compatible with Iron-Hematoxylin staining?

PVA (Polyvinyl-alcohol)

Glutaraldehyde

Bouin’s solution

Formalin

13 / 30

Category: Clinical Laboratory

Which modification uses ferric chloride instead of ferric ammonium sulfate?

Heidenhain’s

Kinyoun’s

Scholten’s

Weigert’s

14 / 30

Category: Clinical Laboratory

How should Schaudinn’s fixative waste be disposed of?

Incineration

Down the sink

Autoclave and landfill

As hazardous waste (mercury content)

15 / 30

Category: Clinical Laboratory

Which solution removes mercuric chloride from fixed smears?

Picric acid

Permount

Xylene

Iodine-alcohol

16 / 30

Category: Clinical Laboratory

What is the key advantage of Iron-Hematoxylin over Trichrome stain?

No need for fixation

Better for helminths

Permanent slides

Faster procedure

17 / 30

Category: Clinical Laboratory

What is the primary purpose of Iron-Hematoxylin staining?

Detecting intestinal protozoan nuclei

Identifying fungal hyphae

Highlighting collagen fibers

Staining bacterial cell walls

18 / 30

Category: Clinical Laboratory

How to test if the working stain is still active?

Add to alkaline tap water—blue = good

Expose to UV light—fluorescence indicates viability

Mix with saline—look for precipitation

Heat to 60°C—check for color change

19 / 30

Category: Clinical Laboratory

Why must staining dishes be covered?

To reduce contamination

To avoid light exposure

To prevent evaporation

All of the above

20 / 30

Category: Clinical Laboratory

Which step follows the hematoxylin staining in the protocol?

Rinsing with tap water

Mounting with permount

Clearing with xylene

Differentiation with picric acid

21 / 30

Category: Clinical Laboratory

Why must Schaudinn’s fixative be thoroughly washed off before staining?

It contains mercury, which interferes with staining

It causes over-oxidation

It dissolves nuclear material

It inhibits hematein formation

22 / 30

Category: Clinical Laboratory

Which step is omitted in the Scholten modification?

Iodine-alcohol

Tap water rinse

Xylene clearing

Acid alcohol differentiation

23 / 30

Category: Clinical Laboratory

What does faint nuclear staining indicate?

Insufficient mordanting

Excessive picric acid

All of the above

Over-oxidation of hematoxylin

24 / 30

Category: Clinical Laboratory

What is the shelf life of stock hematoxylin solution?

6 months

1 month

Indefinite

12 months

25 / 30

Category: Clinical Laboratory

What indicates a failed QC slide?

Rapid staining time

Faded background

Blue tap water test

Poor nuclear contrast

26 / 30

Category: Clinical Laboratory

Which component is NOT in the mordant solution?

Hematoxylin

Ferric ammonium sulfate

Hydrochloric acid

Distilled water

27 / 30

Category: Clinical Laboratory

What is the active dye formed when hematoxylin oxidizes?

Hematein

Eosin

Carbol fuchsin

Oxyhematein

28 / 30

Category: Clinical Laboratory

What causes refractive granules in stained slides?

Contaminated xylene

Over-staining

Incomplete mercury removal

Insufficient drying

29 / 30

Category: Clinical Laboratory

Which component acts as the mordant in Iron-Hematoxylin staining?

Hydrochloric acid

Ferric ammonium sulfate

Ethanol

Hematoxylin

30 / 30

Category: Clinical Laboratory

What is the purpose of the 70% ethanol wash after fixation?

To remove excess fixative

To oxidize hematoxylin

To dehydrate the smear

To counterstain

Your score is

The average score is 44%

Read Full Article: Iron-Hematoxylin Staining 50 FAQ and 30 MCQs

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