Biochemical Test of Pseudomonas Aeruginosa 50 FAQs and 30 MCQs:
Biochemical Test of Pseudomonas Aeruginosa 50 FAQs
Is Pseudomonas aeruginosa Gram-positive or Gram-negative?
Gram-negative.
What is the shape of Pseudomonas aeruginosa?
Rod-shaped (bacilli).
Is Pseudomonas aeruginosa motile?
Yes, it is motile.
How is Pseudomonas aeruginosa typically motile?
Motile via a single polar flagellum (unipolar).
Does Pseudomonas aeruginosa form spores?
No, it is non-sporing.
Does Pseudomonas aeruginosa have a capsule?
There is variation; some strains are non-capsulated, while others secrete an alginate capsule/polysaccharide.
What type of hemolysis does Pseudomonas aeruginosa exhibit?
Beta-hemolysis
Does Pseudomonas aeruginosa produce pigment?
Yes, it produces pigments (typically blue-green pyocyanin/fluorescein).
What is the catalase test result for Pseudomonas aeruginosa?
Positive (+ve).
What is the oxidase test result for Pseudomonas aeruginosa?
Positive (+ve).
What is the citrate utilization test result?
Positive (+ve) – it can use citrate as a sole carbon source.
What is the result of the cetrimide test?
Positive (+ve) – it grows in the presence of cetrimide, a selective agent.
What is the gelatin hydrolysis test result?
Positive (+ve) – it liquefies gelatin.
What is the indole test result?
Negative (-ve).
What is the Methyl Red (MR) test result?
Negative (-ve).
What is the Voges-Proskauer (VP) test result?
Negative (-ve).
What is the urease test result?
Negative (-ve) – it does not hydrolyze urea.
Does Pseudomonas aeruginosa reduce nitrate?
Yes, positive (+ve) for nitrate reduction.
Does Pseudomonas aeruginosa produce gas?
source 1 says Negative (-ve), source 2 says Positive (+ve) from nitrate
What is the H2S production test result?
Negative (-ve).
What is the coagulase test result?
Negative (-ve).
Is Pseudomonas aeruginosa oxidative or fermentative?
Oxidative (OF test).
What is the TSIA (Triple Sugar Iron Agar) reaction?
Alkali/Alkali (Red/Red) – K/K, no fermentation, no H2S.
What is the DNase test result?
Negative (-ve)
Does Pseudomonas aeruginosa ferment Glucose?
Negative (-ve)
Does it ferment Lactose?
Negative (-ve)
Does it ferment Maltose?
Negative (-ve)
Does it ferment Sucrose?
Negative (-ve)
Does it ferment Mannitol?
Positive (+ve
Does it ferment Arabinose?
Negative (-ve)
Does it ferment Fructose?
Positive (+ve)
Does it ferment Sorbitol?
Negative (-ve)
Does it ferment Adonitol?
Negative (-ve)
Does it ferment Inulin?
Negative (-ve)
Does it ferment Galactose?
Negative (-ve)
Does it ferment Cellobiose?
Negative (-ve)
Does it ferment Trehalose?
Negative (-ve)
Does it ferment Xylose?
Negative (-ve)
Does it ferment Ribose?
Positive (+ve)
What is the arginine dihydrolase test result?
Positive (+ve)
What is the lysine decarboxylase test result?
Negative (-ve)
What is the ornithine decarboxylase test result?
Negative (-ve)
What is the phenylalanine deaminase test result?
Negative (-ve)
What is the lipase test result?
Positive (+ve)
What is the lecithinase test result?
Negative (-ve)
What is the esculin hydrolysis test result?
Negative (-ve)
Can Pseudomonas aeruginosa utilize acetate?
Positive (+ve)
What is the malonate utilization test result?
Positive (+ve)
What is the hippurate hydrolysis test result?
Negative (-ve)
What is the ONPG test result?
Not explicitly stated, but inferred negative due to lactose fermentation negativity .
Biochemical Test of Pseudomonas Aeruginosa 30 MCQs
- What is the Gram stain result for Pseudomonas aeruginosa?
a) Gram-positive
b) Gram-negative✔
c) Variable
d) Acid-fast - Which motility structure does P. aeruginosa possess?
a) Peritrichous flagella
b) Lophotrichous flagella
c) Single polar flagellum (unipolar)✔
d) Non-motile - What is the oxidase test result for P. aeruginosa?
a) Positive✔
b) Negative
c) Variable
d) Not tested - Which sugar fermentation is POSITIVE for P. aeruginosa?
a) Lactose
b) Glucose
c) Mannitol✔
d) Sucrose - The Cetrimide test is used to identify P. aeruginosa because it is:
a) Negative
b) Positive✔
c) Variable
d) Inconclusive - What is the gelatin hydrolysis result for P. aeruginosa?
a) Negative
b) Positive✔
c) Slow positive
d) Not documented - How does P. aeruginosa metabolize sugars in the OF (Oxidative-Fermentative) test?
a) Fermentative
b) Oxidative✔
c) Both oxidative and fermentative
d) Neither - Which enzyme test is POSITIVE for P. aeruginosa?
a) Urease
b) Coagulase
c) Arginine dihydrolase✔
d) DNase - What is the citrate utilization result for P. aeruginosa?
a) Negative
b) Positive✔
c) Weakly positive
d) Not applicable - Which hemolytic pattern does P. aeruginosa exhibit?
a) Alpha hemolysis
b) Beta hemolysis✔
c) Gamma hemolysis
d) No hemolysis
- The Triple Sugar Iron Agar (TSIA) reaction for P. aeruginosa is:
a) Acid/Acid (Yellow/Yellow)
b) Alkali/Acid (Red/Yellow)
c) Alkali/Alkali (Red/Red)✔
d) Acid/H2S (Yellow/Black) - Which test is NEGATIVE for P. aeruginosa?
a) Catalase
b) Nitrate reduction
c) Indole✔
d) Lipase - What is the sporulation status of P. aeruginosa?
a) Spore-forming
b) Non-sporing✔
c) Spores under stress only
d) Variable - Which pigment is produced by P. aeruginosa?
a) Red
b) Yellow
c) Blue-green✔
d) Purple - What is the methyl red (MR) test result for P. aeruginosa?
a) Positive
b) Negative✔
c) Weakly positive
d) Not reported - Which sugar is NOT fermented by P. aeruginosa?
a) Fructose
b) Ribose
c) Mannitol
d) Lactose✔ - The coagulase test for P. aeruginosa is:
a) Positive
b) Negative✔
c) Variable
d) Not tested - What is the urease test result for P. aeruginosa?
a) Positive
b) Negative✔
c) Delayed positive
d) Inconclusive - Which enzyme is POSITIVE in P. aeruginosa?
a) Lysine decarboxylase
b) Ornithine decarboxylase
c) Lipase✔
d) Lecithinase - Gas production by P. aeruginosa is associated with:
a) Carbohydrate fermentation
b) Nitrate reduction✔
c) Protein degradation
d) Never produced
- What is the catalase test result for P. aeruginosa?
a) Positive✔
b) Negative
c) Variable
d) Not documented - Which of these carbon sources is utilized by P. aeruginosa?
a) Malonate✔
b) Inositol
c) Sorbitol
d) Adonitol - The Voges-Proskauer (VP) test for P. aeruginosa is:
a) Positive
b) Negative✔
c) Weakly positive
d) Not applicable - Which test is used to selectively isolate P. aeruginosa?
a) Oxidase test
b) Catalase test
c) Cetrimide test✔
d) Coagulase test - What is the H₂S production result for P. aeruginosa?
a) Positive
b) Negative✔
c) Variable
d) Not tested - Fructose fermentation by P. aeruginosa is:
a) Positive✔
b) Negative
c) Variable
d) Not reported - The lysine decarboxylase test for P. aeruginosa is:
a) Positive
b) Negative✔
c) Weakly positive
d) Not documented - Which characteristic describes P. aeruginosa‘s capsule?
a) Always encapsulated
b) Always non-capsulated
c) Some strains produce alginate capsule✔
d) Capsule absent - Ribose fermentation by P. aeruginosa is:
a) Positive✔
b) Negative
c) Variable
d) Not tested - What is the ornithine decarboxylase test result for P. aeruginosa?
a) Positive
b) Negative✔
c) Delayed positive
d) Inconclusive
