Wheatley Trichrome Staining MCQs Mock Test (30 MCQs)

Test your parasitology skills with 30+ Wheatley’s Trichrome MCQs! This mock exam covers:
◼️ Staining mechanism for Giardia trophozoites & cysts
◼️ Troubleshooting faded stains
◼️ Differentiation of E. histolytica vs. E. coli
◼️ CDC-recommended protocols

Learn Wheatley’s Trichrome staining, the gold standard for intestinal parasite identification in stool samples. This guide covers:
✓ Principle behind trichrome staining for protozoa (Giardia, Entamoeba)
✓ Step-by-step protocol with modifications
✓ Color interpretation (cytoplasm vs. nuclear staining)
✓ Comparison with other stains (Iron-Hematoxylin, Iodine)

Essential for clinical microbiologists, parasitologists, and medical lab technicians diagnosing parasitic infections.

Ideal for MLT certification, ASCP exams, and tropical medicine courses.

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Microbiology Department

Wheatley Trichrome Staining MCQs Mock Test

Essential for clinical microbiologists, parasitologists, and medical lab technicians diagnosing parasitic infections.

Read Full Article: Wheatley Trichrome Staining 50 FAQ and 30 MCQs

1 / 30

Category: Clinical Laboratory

What is the recommended storage condition for Trichrome stain?

Frozen at -20°C

Heated at 37°C

Refrigerated at 4°C

Room temperature, protected from light

2 / 30

Category: Clinical Laboratory

What is the purpose of the iodine step in the staining process?

To fix the stool sample

To stain the nuclei

To remove mercuric chloride

To decolorize the smear

3 / 30

Category: Clinical Laboratory

What is the primary limitation of Wheatley Trichrome Stain?

It cannot stain protozoans

It cannot stain helminth eggs and larvae

It cannot stain yeast cells

It cannot stain human cells

4 / 30

Category: Clinical Laboratory

Which of the following is NOT a component of the Trichrome stain solution?

Phosphotungstic acid

Chromotrope 2R

Light Green SF

Methylene blue

5 / 30

Category: Clinical Laboratory

What type of stool sample is used for Wheatley Trichrome staining?

Formalin-preserved only

Fresh or PVA-fixed

Frozen stool

Urine-contaminated stool

6 / 30

Category: Clinical Laboratory

Who originally developed the Trichrome stain?

Ziehl

Neelsen

Gomori

Wheatley

7 / 30

Category: Clinical Laboratory

What causes dark crystalline materials on the smear?

Over-decolorization

Over-staining

Inadequate drying

Saturation of iodine-alcohol solution

8 / 30

Category: Clinical Laboratory

What is the purpose of the mounting medium in the staining process?

To decolorize the smear

To remove iodine

To preserve the stained smear

To fix the smear

9 / 30

Category: Clinical Laboratory

What is the recommended fixative for stool samples in Wheatley Trichrome staining?

PVA or Schaudinn’s fixative

Formalin

Ethanol

Xylene

10 / 30

Category: Clinical Laboratory

What is the shelf life of the Trichrome stain solution?

24 months

6 months

12 months

36 months

11 / 30

Category: Clinical Laboratory

What is the purpose of acid ethanol in the staining process?

To remove iodine

To stain the nuclei

To fix the smear

To decolorize the smear

12 / 30

Category: Clinical Laboratory

What color do human cells like RBCs and PMNs stain?

Blue

Red

Purple

Green

13 / 30

Category: Clinical Laboratory

What is the primary purpose of the Wheatley Trichrome Stain?

Identifying fungal spores

Staining bacterial cells

Staining helminth eggs

Detecting intestinal protozoans

14 / 30

Category: Clinical Laboratory

Which of the following cannot be stained by Wheatley Trichrome Stain?

Helminth eggs

Human cells

Intestinal protozoan cysts

Yeast cells

15 / 30

Category: Clinical Laboratory

What is the recommended drying time for a mounted smear?

1 hour at 37°C

Both a and c

Overnight at room temperature

2 hours at room temperature

16 / 30

Category: Clinical Laboratory

What is the primary purpose of the xylene step in the staining process?

To fix the smear

To stain the nuclei

To clear the slide

To decolorize the smear

17 / 30

Category: Clinical Laboratory

What is the purpose of the acid ethanol step?

To remove iodine

To fix the smear

To stain the nuclei

To differentiate the stain

18 / 30

Category: Clinical Laboratory

What is the primary application of Wheatley Trichrome Stain?

Diagnosing intestinal protozoa infections

Staining bacterial cells

Staining helminth eggs

Identifying fungal spores

19 / 30

Category: Clinical Laboratory

What is the purpose of xylene in the staining process?

To clear the slide

To decolorize the smear

To stain the nuclei

To fix the smear

20 / 30

Category: Clinical Laboratory

What modification did Wheatley make to Gomori’s Trichrome stain?

Eliminated the use of xylene

Reduced staining time to 1 minute

Added fixation and hydration steps

Added iodine-alcohol step

21 / 30

Category: Clinical Laboratory

What is the purpose of the ethanol rinse after decolorization?

To stain the nuclei

To fix the smear

To halt decolorization

To remove iodine

22 / 30

Category: Clinical Laboratory

What color does the background stain in a Wheatley Trichrome smear?

Green

Blue

Red

Purple

23 / 30

Category: Clinical Laboratory

Which protozoan is difficult to identify using Wheatley Trichrome Stain?

Cryptosporidium parvum

Giardia lamblia

Entamoeba histolytica

Trichomonas vaginalis

24 / 30

Category: Clinical Laboratory

How long should slides remain in Trichrome stain?

30 minutes

1 minute

10 minutes

5 minutes

25 / 30

Category: Clinical Laboratory

What happens to glycogen molecules during staining?

They dissolve and appear clear

They stain purple

They stain red

They stain green

26 / 30

Category: Clinical Laboratory

What color do protozoan trophozoites stain?

Green

Yellow

Red

Blue-green or light purple

27 / 30

Category: Clinical Laboratory

What is the purpose of iodine-alcohol in the staining process?

To remove mercuric chloride

To decolorize the smear

To stain the cytoplasm

To fix the stool sample

28 / 30

Category: Clinical Laboratory

What is the primary limitation of Wheatley Trichrome Stain for Cryptosporidium?

It requires acid-fast staining for Cryptosporidium

It cannot stain Cryptosporidium

It over-stains Cryptosporidium

It cannot differentiate Cryptosporidium from yeast

29 / 30

Category: Clinical Laboratory

What is the primary reason for using PVA in stool sample preparation?

To remove iodine

To stain the nuclei

To decolorize the smear

To preserve the stool sample

30 / 30

Category: Clinical Laboratory

What magnification is used to examine the stained smear?

1000x

100x (oil immersion)

400x

40x

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Read Full Article: Wheatley Trichrome Staining 50 FAQ and 30 MCQs

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