Martin Lewis Agar 30 MCQs Mock Test for Exam

Welcome to the Martin Lewis Agar Mock Test, designed to help students, laboratory technologists, and microbiology professionals assess their understanding of this important selective culture medium.

Martin Lewis Agar is widely used in diagnostic microbiology for the isolation of Neisseria gonorrhoeae and Neisseria meningitidis from clinical specimens that may contain a variety of contaminating microorganisms. Knowledge of its composition, antimicrobial supplements, principle of selectivity, and laboratory applications is essential for academic success and professional practice.

This mock test contains 30 carefully designed questions that cover key concepts and practical applications of Martin Lewis Agar. Use this assessment to measure your knowledge, identify learning gaps, and improve your confidence in clinical microbiology.

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Martin Lewis Agar 30 MCQs Mock Test for Exam

Martin Lewis Agar MCQs Mock Test

Please read before starting the Martin Lewis Agar – 30 MCQs Mock Test. This test includes 30 multiple-choice questions, each with one correct answer. There is no negative marking, so attempt all questions. Avoid refreshing or closing the page to prevent losing your progress. After answering all questions, you may review your responses before submitting. If you want a Certificate, enter your correct Full Name and active Email after completing the test and before submission. Your certificate can be downloaded directly from the result page once the test is submitted.

Read Full Article: Martin Lewis Agar 50 FAQs and 30 MCQS

1 / 30

Category: Clinical Laboratory

What maintains the pH in Martin Lewis Agar?

Hemoglobin

Dextrose

Phosphate buffer

Sodium chloride

2 / 30

Category: Clinical Laboratory

What is the role of corn starch in the agar?

Nutrient source

Indicator of pH

Inhibit gram-positives

Neutralize toxic fatty acids

3 / 30

Category: Clinical Laboratory

What does L-cysteine in Bio-X enrichment provide?

Carbohydrate source

Reducing agent

Vitamin B12

Iron ions

4 / 30

Category: Clinical Laboratory

How is CO₂ generated in Hardy’s Martin Lewis Agar?

GasPak system

Incubator settings

Candle jar

Sodium bicarbonate and citric acid tablet

5 / 30

Category: Clinical Laboratory

Which Neisseria species is used for QC of Martin Lewis Agar?

N. lactamica

N. sicca

N. subflava

N. gonorrhoeae ATCC 43069

6 / 30

Category: Clinical Laboratory

Which component in Bio-X provides ferric ions?

Adenine

Dextrose

Ferric nitrate

L-glutamine

7 / 30

Category: Clinical Laboratory

Which antibiotic was added to Thayer-Martin Agar but not Martin Lewis?

Vancomycin

Nystatin

Anisomycin

Colistin

8 / 30

Category: Clinical Laboratory

How do colonies of Neisseria gonorrhoeae appear on Martin Lewis Agar?

Small grayish-white mucoid colonies

Translucent with green hemolysis

Yellow pigmented colonies

Large blue-gray colonies

9 / 30

Category: Clinical Laboratory

What temperature should the agar base be cooled to before adding hemoglobin and antibiotics?

70°C

37°C

45–50°C

25°C

10 / 30

Category: Clinical Laboratory

Which antibiotic in Martin Lewis Agar targets fungi?

Colistin

Vancomycin

Anisomycin

Trimethoprim

11 / 30

Category: Clinical Laboratory

What is the sterilization method for the agar base?

UV radiation

Filtration

Boiling for 30 minutes

Autoclaving at 121°C for 15 minutes

12 / 30

Category: Clinical Laboratory

Why might some Neisseria gonorrhoeae strains not grow on Martin Lewis Agar?

Lack of X factor

Absence of dextrose

Inhibition by vancomycin

High pH

13 / 30

Category: Clinical Laboratory

How should dehydrated Martin Lewis Agar be stored?

At room temperature in open air

Exposed to light

In a freezer

Below 30°C in a sealed container

14 / 30

Category: Clinical Laboratory

How should inoculated plates be transported?

Ambient temperature (15-30°C)

At 2-8°C

Frozen

Heated to 37°C

15 / 30

Category: Clinical Laboratory

After isolation on Martin Lewis Agar, what test is typically performed first?

Catalase test

Oxidase test

Gram stain

Coagulase test

16 / 30

Category: Clinical Laboratory

What color is prepared Martin Lewis Agar?

Chocolate-colored opaque

Transparent

Red

Green

17 / 30

Category: Clinical Laboratory

Which organism’s swarming is inhibited on Martin Lewis Agar?

Candida albicans

Staphylococcus aureus

Escherichia coli

Proteus mirabilis

18 / 30

Category: Clinical Laboratory

Which antibiotic in Martin Lewis Agar inhibits gram-positive bacteria?

Vancomycin

Colistin

Trimethoprim

Anisomycin

19 / 30

Category: Clinical Laboratory

Which organism is used for QC of Martin Lewis Agar?

Escherichia coli ATCC 25922

Streptococcus pyogenes

Lactobacillus acidophilus

Mycobacterium tuberculosis

20 / 30

Category: Clinical Laboratory

What is the primary purpose of Martin Lewis Agar?

Culturing fungi

Growing anaerobic bacteria

Detecting viral infections

Isolating Neisseria species

21 / 30

Category: Clinical Laboratory

How should prepared Martin Lewis Agar plates be stored?

In a desiccator

At room temperature in light

Refrigerated at 2–8°C

Frozen at -20°C

22 / 30

Category: Clinical Laboratory

What is the role of lincomycin in Hardy’s variant?

Inhibit gram-negative bacteria

Provide V factor

Replace vancomycin to reduce inhibition

Enhance fungal growth

23 / 30

Category: Clinical Laboratory

Which antibiotic in Martin Lewis Agar inhibits Proteus species?

Trimethoprim

Colistin

Vancomycin

Anisomycin

24 / 30

Category: Clinical Laboratory

What atmosphere is required for incubating Martin Lewis Agar?

Microaerophilic

Anaerobic

Ambient air

5-10% CO₂

25 / 30

Category: Clinical Laboratory

Which component provides the X factor in Martin Lewis Agar?

Bio-X enrichment

Dextrose

Hemoglobin

Vancomycin

26 / 30

Category: Clinical Laboratory

Which organism is NOT inhibited by Martin Lewis Agar?

Candida albicans

Staphylococcus epidermidis

Proteus species

Neisseria gonorrhoeae

27 / 30

Category: Clinical Laboratory

How do Neisseria meningitidis colonies appear on Martin Lewis Agar?

Yellow and opaque

Red and dry

Blue-gray and mucoid

Small and white

28 / 30

Category: Clinical Laboratory

What addition in Hardy’s Martin Lewis Agar aids recovery of oropharyngeal Neisseria?

Higher starch content

Bovine serum

Penicillin

Lincomycin and CO₂ tablets

29 / 30

Category: Clinical Laboratory

How does Martin Lewis Agar differ from Thayer-Martin Agar?

Higher vancomycin to inhibit gram-positive bacteria

Lacks antibiotics

Contains lower vancomycin

Uses human blood instead of sheep blood

30 / 30

Category: Clinical Laboratory

Which component supplies the V factor (NAD) in Martin Lewis Agar?
a) Hemoglobin
b) Bio-X enrichment✔
c) Vancomycin
d) Corn starch

Vancomycin

Hemoglobin

Corn starch

Bio-X enrichment

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Final Thoughts

Completing this Martin Lewis Agar Mock Test is an excellent way to reinforce your understanding of selective culture media and the laboratory diagnosis of Neisseria infections. Consistent practice with microbiology assessments can improve retention, boost examination performance, and enhance practical laboratory competency.

Continue exploring additional microbiology mock tests and culture media quizzes to build a stronger foundation in clinical microbiology and diagnostic laboratory science.

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