Auramine- Rhodamine Staining MCQs Mock Test (30 MCQs)

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Auramine-Rhodamine Stain Guide: Learn fluorescent AFB detection for TB diagnosis – protocol, interpretation, and advantages over ZN staining. Auramine-Rhodamine Quiz: 35+ MCQs on fluorescent AFB staining for TB diagnosis – perfect for lab tech certification prep!

Master Auramine-Rhodamine staining, the sensitive fluorescent technique for rapid acid-fast bacilli (AFB) screening in tuberculosis diagnosis. This guide covers:

✓ Dual-fluorochrome principle (Auramine O & Rhodamine B)
✓ CDC-approved protocol for sputum smears
✓ Key advantages over Ziehl-Neelsen staining:

  • 10x higher sensitivity
  • Faster screening (40x vs 100x magnification)
  • Better for paucibacillary samples

✓ Microscopy interpretation:

  • Apple-green fluorescing rods = AFB
  • Red-orange counterstain = background

✓ Quality control measures
✓ Comparison with LED fluorescence microscopy

Essential for TB lab technicians, microbiologists, and public health workers in high-burden settings.

Test your TB diagnostics skills with 35+ Auramine-Rhodamine MCQs:
◼️ Principle of fluorochrome binding
◼️ Correct filter combinations
◼️ CDC grading criteria (1+ to 4+)
◼️ Biosafety considerations

Crucial for:

  • NTEP/RNTCP certification (India)
  • WHO TB laboratory training
  • MLS/MLT board exams
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Microbiology Department

Auramine- Rhodamine Staining MCQs Mock Test

Auramine- Rhodamine Staining MCQs Mock Test

Test your TB diagnostics skills with 30 Auramine-Rhodamine MCQs:
◼️ Principle of fluorochrome binding
◼️ Correct filter combinations
◼️ CDC grading criteria (1+ to 4+)
◼️ Biosafety considerations

Crucial for:

  • NTEP/RNTCP certification (India)

  • WHO TB laboratory training

  • MLS/MLT board exams

Read Full Article: Auramine- Rhodamine Staining FAQs and MCQs

1 / 30

Category: Clinical Laboratory

Which organization recommends fluorochrome staining for AFB detection?

2 / 30

Category: Clinical Laboratory

What is the major drawback of potassium permanganate?

3 / 30

Category: Clinical Laboratory

 What PPE is required when handling these stains?

4 / 30

Category: Clinical Laboratory

Why is distilled water preferred over tap water for rinsing?

5 / 30

Category: Clinical Laboratory

What type of microscope is required for Auramine-Rhodamine staining?

6 / 30

Category: Clinical Laboratory

Why is heat fixation avoided during staining?

7 / 30

Category: Clinical Laboratory

What is the alternative staining method if a fluorescent microscope is unavailable?

8 / 30

Category: Clinical Laboratory

What does a pale yellow background indicate?

9 / 30

Category: Clinical Laboratory

A major limitation of Auramine-Rhodamine staining is:

10 / 30

Category: Clinical Laboratory

 Why is culture still needed after a positive stain?

11 / 30

Category: Clinical Laboratory

How many fields should be examined before reporting a negative result at 400X magnification?

12 / 30

Category: Clinical Laboratory

 What is the primary purpose of Auramine-Rhodamine staining?

13 / 30

Category: Clinical Laboratory

 How should acid-alcohol waste be disposed of?

14 / 30

Category: Clinical Laboratory

Which reagent acts as the decolorizer in Auramine-Rhodamine staining?

15 / 30

Category: Clinical Laboratory

What is the role of potassium permanganate in this staining?

16 / 30

Category: Clinical Laboratory

How should a negative result be confirmed?

17 / 30

Category: Clinical Laboratory

What happens if the counterstain (potassium permanganate) is left too long?

18 / 30

Category: Clinical Laboratory

Which specimen is NOT suitable for Auramine-Rhodamine staining?

19 / 30

Category: Clinical Laboratory

A positive Auramine-Rhodamine stain shows AFB as:

20 / 30

Category: Clinical Laboratory

Why must slides be examined within 24 hours?

21 / 30

Category: Clinical Laboratory

Which component of Mycobacterium spp. binds to Auramine-Rhodamine dye?

22 / 30

Category: Clinical Laboratory

 Which of the following is an advantage of Auramine-Rhodamine over ZN staining?

23 / 30

Category: Clinical Laboratory

How long should the primary stain (Auramine-Rhodamine) be applied?

24 / 30

Category: Clinical Laboratory

Which reagent in Auramine-Rhodamine staining is a possible carcinogen?

25 / 30

Category: Clinical Laboratory

What is the recommended filter combination for fluorescence microscopy?

26 / 30

Category: Clinical Laboratory

What happens if the smear is too thick?

27 / 30

Category: Clinical Laboratory

If AFB appear faint, what could be the reason?

28 / 30

Category: Clinical Laboratory

What color do acid-fast bacilli (AFB) appear under fluorescence?

29 / 30

Category: Clinical Laboratory

Why is Auramine-Rhodamine staining more sensitive than Ziehl-Neelsen (ZN) staining?

30 / 30

Category: Clinical Laboratory

Which organism may NOT fluoresce with this stain?

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Read Full Article: Auramine- Rhodamine Staining FAQs and MCQs

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