One of the ways I protect paraffin slides is to dip in hot paraffin and store at 4 degrees C in a air tight box. Certain antigens can degrade within 30 days, especially estrogen and progesterone.
Tag: histopathology techniques
Unstained paraffin sections offer very low contrast and therefore cannot be evaluated microscopically in routine histopathology. It is necessary to apply coloring reagents (mostly chemicals) to stain tissue structures.
The objective of this step is to cut 4–5 Mm-thick sections from paraffin blocks. This is achieved using precision knives (microtomes) .
Once tissue samples are infiltrated by paraffin, they are removed from the cassettes and carefully positioned inside a metal base mold
After fixation, tissue samples need to be properly trimmed to reach the adequate size and orientation of the tissue.
Histology is the study of tissues, and pathology is the study of disease. So taken together, histopathology literally means the study of tissues as relates to disease. Histopathology (or histology) involves the examination of sampled whole tissues under the microscope. Three main types of specimen are received by the pathology laboratory. Specimens received by the pathology laboratory require tissue preparation then are treated and analysed using techniques appropriate to the type of tissue and the […]
Fixation is a process in which tissue & cells are preserved in a state as close to life as possible. No, micro & macro structures should be lost.
The technique of getting fixed tissue into paraffin is called tissue processing. Tissue processing is designed to remove all extractable water from the tissue, replacing it with a support medium that provides sufficient rigidity to enable sectioning of the tissue without damage or distortion