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Tag: h&e stain

Periodic acid-Schiff’s (PAS) Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

PAS Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

H&E Stain

H & E Stain

Hematoxylin and eosin staining technique functions to recognize different types of tissues and their morphological changes, especially in cancer diagnosis. Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction.

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