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Tag: h&e stain

Periodic acid-Schiff’s (PAS) Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

PAS Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

Hematoxylin and Eosin (H&E) Stain

The most common routine staining combination. Hematoxylin is used in combination with metal ions, such as aluminum or iron, to form colored chelate complexes. The ingredients of the stain act as cations and are preferentially bound to acidic (anionic) groups in the tissues. The structures to which hematoxylin binds are termed “basophilic” (base-loving). Hematoxylin stains regions rich in nucleic acids blue. Eosin stains many structures (collagen and some areas of cytoplasm) pink. These areas have […]

H&E Stain

The most common routine staining combination. Hematoxylin is used in combination with metal ions, such as aluminum or iron, to form colored chelate complexes. The ingredients of the stain act as cations and are preferentially bound to acidic (anionic) groups in the tissues. The structures to which hematoxylin binds are termed “basophilic” (base-loving). Hematoxylin stains regions rich in nucleic acids blue. Eosin stains many structures (collagen and some areas of cytoplasm) pink. These areas have […]

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