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Coagulase Test

Coagulase test is used to differentiate Staphylococcus aureus (positive) from Coagulase Negative Staphylococcus (CONS). Coagulase is an enzyme produced by S. aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin. Staphylococcus aureus produces two forms of coagulase, bound and free. Slide coagulase test is done to detect bound coagulase or clumping factor. Tube coagulase test is done to detect free coagulase. Coagulation Test (Slide Method) Emulsify a staphylococcal colony in a drop of water […]

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