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Tag: alcian blue stain

Metachromasia Stain

The property of certain biological compounds to change the color of such dyes as toluidine blue or thionine. For example, glycosaminoglycans/proteoglycans found in cartilage matrix and mast cell granules will stain red or violet instead of blue with toluidine blue. The phenomenon is apparently caused by a change in the electronic structure of the dye molecule as it interacts with the electrons of the binding polymer and other dye molecules.

Periodic acid-Schiff’s (PAS) Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

PAS Stain

Leucofuchsin (Schiff reagent) has become one of the most useful of histochemical dyes. It is colorless due to prior reduction of the basic fuchsin; it then may be used under varied conditions to stain compounds that are capable of recolorizing the leucofuchsin by oxidation. It serves as a specific stain for deoxyribonucleic acid after hydrolysis of the tissue section with HCl (Feulgen reaction). More generally, it is employed with the “PAS” reaction after oxidation with […]

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